A strain of Bacillus megaterium, its polypeptide agricultural microbial agent, its preparation method and application

A technology of Bacillus megaterium and microbial agent, applied in the field of microbial fertilizers, can solve the problems of organic nutrient loss of active bacteria, high energy consumption of liquid fermentation, and single active peptide form, so as to improve absorption and utilization efficiency and improve micro-ecological environment , the effect of rich nutrient content

Active Publication Date: 2021-02-12
KINGENTA ECOLOGICAL ENG GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polypeptide fertilizer obtained by acid hydrolysis has the following disadvantages: 1) it needs to be carried out in an autoclave under high temperature and high pressure conditions, which has high cost and poor safety; 2) it needs to use a large amount of acid and alkali in the production process, which is not conducive to environmental protection; 3) acid The active peptide obtained by hydrolysis of protein has a single form and limited biological activity. Most of the configurations of amino acids obtained by hydrolysis change, which cannot be absorbed and utilized by crops, and the fertilizer efficiency is low.
It uses Bacillus subtilis with high protease production, Bacillus licheniformis with high amylase production and black mold fermentation cake to produce soybean polypeptide, and produces plant polypeptide and series of products through microbial deep layer, homogeneous and liquid fermentation technology. Although the amino acid produced by the above invention- Although polypeptide liquid fertilizer has a high content of polypeptide in amino acid-polypeptide liquid fertilizer, the production process is cumbersome, the energy consumption of liquid fermentation is high, and the subsequent filtration process is added for solid-liquid separation and product extraction, and the active functional bacteria in the fermentation process are filtered out. , some incompletely degraded organic nutrients are also filtered into the solid filter residue, resulting in a large loss of active bacteria and organic nutrients, thereby reducing fertilizer efficiency

Method used

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  • A strain of Bacillus megaterium, its polypeptide agricultural microbial agent, its preparation method and application
  • A strain of Bacillus megaterium, its polypeptide agricultural microbial agent, its preparation method and application
  • A strain of Bacillus megaterium, its polypeptide agricultural microbial agent, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:

[0065] (1) Preparation of functional bacteria Bacillus subtilis seed liquid

[0066] A. Activate the Bacillus subtilis strain p-1 preserved at low temperature on the LB plate medium, pick a single colony and culture it on the LB medium slant medium at 37° C. for 24 hours to obtain the activated bacterial strain;

[0067] B. Use a sterile inoculation loop to scrape a loop of the strain activated in step A and inoculate it into 50ml of liquid seed medium, and cultivate it at 35°C for 18 hours to obtain the Bacillus subtilis seed solution. The number of viable bacteria in the seed solution is 3.8*10 9 CFU / ml.

[0068] (2) Preparation of functional bacteria Bacillus megaterium seed solution

[0069]a. Activate the Bacillus megaterium strain HB stored at low temperature on LB plate medium, pick a single colony and culture it on LB slant medium at 37°C for ...

Embodiment 2

[0079] Embodiment 2: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:

[0080] (1) The preparation of the functional bacteria Bacillus subtilis seed liquid is the same as in Example 1, and the number of viable bacteria in the seed liquid is 3.2*10 9 CFU / ml.

[0081] (2) The preparation of the functional bacterium Bacillus megaterium seed liquid is the same as in Example 2, and the number of viable bacteria in the seed liquid is 2.2*10 9 CFU / ml.

[0082] (3) Preparation of mixture

[0083] In parts by mass, take 80 parts of soybean meal powder, 5 parts of peanut straw powder, 5 parts of wheat bran, 5 parts of distiller's grains, and 5 parts of corn flour, put them into a container and mix them evenly, add water to adjust to a moisture content of 70%, and store at 28°C Stand for 24 hours; cook at 121°C for 20 minutes to obtain the mixture, and cool it for later use;

[0084] (4) Preparation of fermented product

[0085] ...

Embodiment 3

[0090] Embodiment 3: a kind of preparation method of polypeptide agricultural microbial bacterial agent, the steps are as follows:

[0091] (1) The preparation of the functional bacteria Bacillus subtilis seed liquid is the same as in Example 1, and the number of viable bacteria in the seed liquid is 3.72*10 9 CFU / ml.

[0092] (2) The preparation of the functional bacterium Bacillus megaterium seed liquid is the same as in Example 2, and the number of viable bacteria in the seed liquid is 2.89*10 9 CFU / ml.

[0093] (3) Preparation of mixture

[0094] In parts by mass, take 65 parts of soybean meal powder, 15 parts of corn stalk powder, 5 parts of wheat bran, 10 parts of distiller's grains, and 5 parts of corn flour, put them into a container and mix them evenly, add water to adjust the moisture content to 65%, and store at 30°C Stand for 20 hours; cook at 121°C for 25 minutes to obtain the mixture, and cool it for later use;

[0095] (4) Preparation of fermented product

...

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Abstract

The invention relates to a strain of Bacillus megaterium, its polypeptide agricultural microbial agent, a preparation method and application thereof. The present invention uses distiller's grains rich in protein content, soybean meal powder, and straw powder as main raw materials, together with corn flour, wheat bran, and inorganic salts and a small amount of carbon source, after rational compounding, it is steamed; Utilize Bacillus megaterium to decompose different proteins in the raw material under specific fermentation conditions, and make an agricultural microbial bacterial agent with active peptides, amino acids, polysaccharides, etc. as high-efficiency ingredients. , a variety of active peptides, polysaccharides, rich in nutrients, high bacterial activity, and a shelf life of more than 2 years. After field experiments, good quality improvement and production increase effects have been achieved.

Description

technical field [0001] The invention relates to a strain of Bacillus megaterium, its polypeptide agricultural microbial agent, a preparation method and application thereof, and belongs to the technical field of microbial fertilizers. Background technique [0002] Amino acid (amino acid) is a general term for a class of organic compounds containing amino and carboxyl groups. It is the basic unit of biological functional macromolecular proteins and an organic compound containing basic amino groups and acidic carboxyl groups. Amino acids endow proteins with specific molecular structural forms, making his molecules biochemically active. As a substance that can be absorbed and utilized by plants, L-amino acid can supplement the essential amino acids of crops, stimulate and regulate the rapid growth of plants, and promote the absorption of nutrients. Improve crop quality and crop disease resistance; it can also chelate trace elements and promote the absorption and transportation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C05G3/80C05G3/00C12R1/11
CPCC05F5/002C05G3/00C12N1/20C12P1/04C05G3/80C12N1/205C12R2001/11C05F5/008C05F11/00C05F11/08
Inventor 邢芳芳胡兆平范玲超高明夫
Owner KINGENTA ECOLOGICAL ENG GRP
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