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Stable antiheparin lipase detection kit

A detection kit and kit technology, applied in the field of clinical medical detection, can solve the problems of weak anti-interference ability, poor reagent stability, and inability to detect heparin samples, etc., and achieve the effect of strong anti-interference ability, low price, and good stability

Inactive Publication Date: 2018-03-09
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention prepares a stable anti-heparin lipase detection kit, which largely solves some problems exposed by similar products on the market, such as: poor reagent stability, weak anti-interference ability, and inability to detect heparin samples , the cost of reagents is high, etc.

Method used

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  • Stable antiheparin lipase detection kit
  • Stable antiheparin lipase detection kit
  • Stable antiheparin lipase detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Reagent R1 consists of:

[0045] HEPES buffer 0.1mol / L

[0046] Sodium taurodeoxycholate 25mmol / L

[0047] Potassium chloride 9g / L

[0048] Chloroform 2.5ml / L

[0049] Protamine Sulfate 7.5g / L

[0050] Dextran-20000 2g / L

[0051] Triton X-405 1%.

[0052] Reagent R2 consists of:

[0053] Tartrate buffer 0.1mol / L

[0054] Co-lipase 300U / L

[0055] 6'-Methyl resorufin 0.2mol / L

[0056] proclin-300 1.5ml / L

[0057] Dextran-2000 5g / L

[0058] Glycerol 5.5ml / L.

Embodiment 2

[0060] HEPES buffer 0.1mol / L

[0061] Sodium taurodeoxycholate 50mmol / L

[0062] Potassium chloride 9g / L

[0063] Chloroform 2.5ml / L

[0064] Protamine Sulfate 5g / L

[0065] Dextran-20000 2g / L

[0066] Triton X-405 1%.

[0067] Reagent R2 consists of:

[0068] Tartrate buffer 0.1mol / L

[0069] Co-lipase 500KU / L

[0070] 6'-Methyl resorufin 0.2mol / L

[0071] proclin-300 1.5ml / L

[0072] Dextran-2000 5g / L

[0073] Glycerol 10ml / L.

Embodiment 3

[0075] HEPES buffer 0.1mol / L

[0076] Sodium taurodeoxycholate 20-50mmol / L

[0077] Potassium chloride 9g / L

[0078] Chloroform 4ml / L

[0079] Protamine Sulfate 10g / L

[0080] Dextran-20000 5g / L

[0081] Triton X-405 1%.

[0082] Reagent R2 consists of:

[0083] Tartrate buffer 0.1mol / L

[0084] Co-lipase 400KU / L

[0085] 6'-Methyl resorufin 0.2mol / L

[0086] proclin-300 1.5ml / L

[0087] Dextran-2000 5g / L

[0088] Glycerol 7.5ml / L.

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Abstract

The invention discloses an enzymatic colorimetric lipase detection kit. The invention prepares a stable antiheparin lipase detection kit. The stable antiheparin lipase detection kit is a liquid-type double-reagent diagnostic detection kit mainly comprising a reagent R1 and a reagent R2, wherein chloroform, glucan and protamine sulfate are introduced into the reagent R1 on the basis of the conventional formula, glucan and glycerol are introduced into the reagent R2, and qualitative change of the reagent properties are achieved through the newly introduced substances; after introduction of a newformula, the lipase detection kit can eliminate interference on a result in the presence of heparin and bilirubin, and through introduction of a glucan series and the glycerol, the stability of the reagents is greatly enhanced. According to the stable antiheparin lipase detection kit provided by the invention, the stability of the reagents is enhanced, the anti-interference ability of the reagents is greatly improved, and raw materials related in the reagents are conventional raw materials, so that a condition is created for mass collocation and production of the reagents.

Description

[0001] The invention prepares a stable anti-heparin lipase detection kit, which mainly relates to the field of clinical medical detection, in particular to the clinical detection of lipase content in human body. Background technique [0002] Lipase is one of the main enzymes for hydrolyzing oil. Generally speaking, natural oil is the main substrate of lipase. When lipase acts on oil, its main function is to hydrolyze the oil bond connected by fatty acid and glycerol in oil. This is different from other hydrolysis Enzymatic hydrolysis is different. The lipase catalysis system is a heterogeneous system. The catalysis of water-soluble enzymes occurs at the interface of water-insoluble substrates and water. The mechanism of catalysis at this interface is not very clear and cannot be simply explained by the Michaelis-Menten theory. Mechanism of reaction between enzyme and substrate. Some people put forward the hypothesis that lipase is localized on the oil-water interface, and put...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 甘宜梧胡晓飞谭柏清李建营王进王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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