Second harmonic microscopic imaging system based on Bessel beam pulse shaping

Abstract

The invention relates to a second harmonic microscopic imaging system based on Bessel beam pulse shaping. A femtosecond pulse laser transmits femtosecond pulse lasers with energy in Gaussian distribution to a space light isolator by virtue of a half wave plate and a Glan prism sequentially, lights emergent from the space light isolator are transmitted to a first adjustable diaphragm, lights emergent from the first adjustable diaphragm are transmitted to a cone lens to be converted into Bessel lights, the Bessel lights emergent from the cone lens are transmitted to a second adjustable diaphragm, lights emergent from the second adjustable diaphragm are transmitted to a third adjustable diaphragm by virtue of a achromatic lens assembly, lights emergent from the third adjustable diaphragm arescanned and transmitted to a telecentric conjugated system by virtue of a scanning galvanometer assembly, lights emergent from the telecentric conjugated system are transmitted to another half wave plate, light emergent from another half wave plate are transmitted to a dichroscope by virtue of the telecentric conjugated system, lights emergent from the dichroscope are focused on a sample on an objective table by virtue of a microscope objective, the sample produces a second harmonic signal, and then the second harmonic signal is collected by virtue of the microscope objective and then is acquired by an EMCCD by virtue of the dichroscope and a light filter.

Description

technical field [0001] The invention relates to a second harmonic microscopic imaging system, in particular to a second harmonic microscopic imaging system based on Bessel beam pulse shaping, and relates to the technical field of microscopic imaging. Background technique [0002] Optical microscopes have the advantages of intuition and convenient sample handling when observing objects, and optical resolution is an important criterion to measure whether an optical microscope system is excellent. With the development of laser technology, the microscopic imaging system using laser as light source has been greatly developed, and its resolution has been greatly improved. At present, technologies based on the principle of fluorescence imaging have made great progress, such as Structured Illumination Microscopy (SIM), Stimulated Emission Depletion Microscopy (STED) and single-molecule localization and Single Molecule Localization and Composition (Single Molecule Localization and C...

AI Technical Summary

Problems solved by technology

However, the second-harmonic microscopes built in the past have not been able to shape the energy of the incident pulse laser well. The energy distribution of the laser used is generally Gaussian distribution with a certain emission angle. Therefore, when passing through the optical components and the focus of the objective lens Produces obvious diffraction phenomenon, which seriously affects the resolution of imaging

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