Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Device for capturing circulating tumor cells by combining functionalized microspheres with filtering chip and application of device

A tumor cell and chip technology, applied in the field of modern medicine, can solve the problems of low efficiency, unfavorable hysteresis of tissue biopsy, high cost, etc., and achieve the effect of improving the capture ability

Active Publication Date: 2018-02-23
ZHEJIANG UNIV
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The heterogeneity of circulating tumor cells makes it impossible for tissue biopsy to reliably analyze tumor cells. In addition, tissue biopsy may increase the risk of tumor metastasis, and the hysteresis of tissue biopsy is also extremely unfavorable.
[0003] The capture of circulating tumor cells based on specific antibodies on the surface of circulating tumor cells has low efficiency and high cost, making it difficult to effectively promote and apply clinically

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device for capturing circulating tumor cells by combining functionalized microspheres with filtering chip and application of device
  • Device for capturing circulating tumor cells by combining functionalized microspheres with filtering chip and application of device
  • Device for capturing circulating tumor cells by combining functionalized microspheres with filtering chip and application of device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Synthesis of Example 1 Nano Zinc Oxide Modified Microspheres

[0074] Polystyrene microspheres and SiO 2 The steps of synthesizing nano zinc oxide on the surface of microspheres are as follows:

[0075] (1) Cleaning of the microspheres, pipette 200 μl of polystyrene microspheres with a diameter of 23 μm and silica microspheres of 13 μm, and wash with ultrapure water for 3 times, 10 min each time.

[0076] (2) Microsphere pretreatment: place the cleaned microspheres on the surface of a slide and wash them on a plasma cleaner for 180 s.

[0077] (3) Adhere the treated microspheres obtained in step (1) to the seed layer, shake well in the seed layer for 10 seconds, centrifuge, put them back into the seed solution and repeat once, centrifuge the microspheres and rinse with ultrapure water Wash once.

[0078] (4) Put the microspheres covering the seed crystal layer into a 1ml EP tube, and put them in the growth solution at 95°C to synthesize 4H. In this experiment, silic...

experiment example 2

[0083] Preparation of Experimental Example 2 Filter Chip

[0084] (1) Acetone, ethanol, and water were used to ultrasonically clean the 400-micron low-resistance silicon wafer, each time for 10 minutes;

[0085] (2) After drying, the silicon surface is oxidized into silicon dioxide oxide, the thickness of silicon dioxide is 2 μm, the glue is applied by a glue machine to 1.5 μm, the ultraviolet lithography is 5s, the silicon dioxide is etched by RIE, and the deep silicon is etched by STS Machine dry etching silicon 65 microns, acetone degum.

[0086] (3) Keep the etching template obtained in (2) as clean and flat as possible. After cleaning with ethanol and experimental ultrapure water, silanize it for 10 minutes.

[0087] (4) 10g of polydimethylsiloxane was mixed evenly at a ratio of 1:10, poured on the surface of the silicon template, vacuumed for 30 minutes, transferred to a 95-degree hot plate and heated for 60 minutes.

[0088] (5) Use a scalpel to cut the PDMS template ...

Embodiment 4

[0099] The performance test of cell sorting and enrichment in the cell line of embodiment 4

[0100] (1) Four tumor cell lines MCF-7, Bxpc-3, panc-1, and HeLa are used as cell models in Filter CHIP, and the ratio of microspheres to tumor cells is 10 2 , to test the capture efficiency of circulating tumor cells at different flow rates.

[0101] The results of this experiment are as Figure 5 As shown, the capture efficiency of circulating tumor cells at a flow rate of 1ml.h -1 to 3ml.h -1 In the range of , as the speed increases, the capture efficiency does not decrease but increases. This is because the flow rate is too low, and a stable turbulent fluid cannot travel inside the chip. The capture efficiency of tumor cells is limited, and the cells in the fluid with a low flow rate cannot be effectively trapped. After the flow rate was greater than 3ml.h-1, the cell capture efficiency decreased.

[0102] (2) The purity of circulating tumor cells captured by microfluidics is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a device for capturing circulating tumor cells by combining functionalized microspheres with a filtering chip and application of the device. The device comprises a micro-infusion pump, a blood sample to be sorted and enriched and the filtering chip, wherein the filtering chip is composed of a first circular-arc-shaped channel and a second circular-arc-shaped channel; each circular-arc-shaped channel is internally provided with a fence structure; nano zinc oxide modified microspheres and treated blood are mixed to form the blood sample to be sorted and enriched; a sampleinlet of the filtering chip is connected with the micro-infusion pump; the blood sample to be sorted and enriched is injected to the sample inlet of the filtering chip through the micro-infusion pump; leukocytes and other impurities have a small volume and are separated through the fence structure, and the circulating tumor cells are captured. The invention provides the device for capturing the circulating tumor cells by combining the functionalized microspheres with the filtering chip and the application of the device, and the device is used for liquid biopsy; the capturing of the circulating tumor cells is carried out through a virtual inertial force based on the size of the circulating tumor cells and surface antigen integration of the circulating tumor cells.

Description

technical field [0001] The invention belongs to the field of modern medicine, and in particular relates to a device for capturing circulating tumor cells, which is used for liquid biopsy with an energy-activated microsphere combined with a filter chip and its application. Background technique [0002] Liquid biopsy is one of the directions that is getting more and more attention in the field of modern medicine. Compared with tissue biopsy, liquid biopsy can analyze the circulating tumor cells of cancer patients with low trauma, high efficiency and accuracy. Metastatic cancer cells, namely circulating tumor cells (CTCs), play a key role in cancer metastasis. The heterogeneity of circulating tumor cells makes it impossible to reliably analyze tumor cells by tissue biopsy. In addition, tissue biopsy may increase the risk of tumor metastasis, and the hysteresis of tissue biopsy is also extremely unfavorable. [0003] The capture of circulating tumor cells based on specific anti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/00C12M1/12C12N5/09
CPCC12M23/16C12M29/04C12N5/0693
Inventor 王本苏逸
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com