Extraction and purification method for high-purity purple sweet potato anthocyanin
A purple potato anthocyanin and purification method technology, applied in the field of high-purity purple potato anthocyanin extraction and purification, can solve the problem of low upgrading, and achieve the effects of reducing solvent consumption, shortening extraction time, and improving product purity
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Embodiment 1
[0053] Such as figure 1Shown, present embodiment adopts 500kg purple potato to extract anthocyanin example, adopts the following steps:
[0054] (1) Cleaning and peeling
[0055] Take 500kg of fresh purple sweet potatoes, wash and remove stones with a paddle cleaner, and then transfer them to a brush cleaning and peeling machine for deep cleaning and peeling.
[0056] (2) Filing and grinding
[0057] After the peeled purple sweet potato is preliminarily crushed by a crusher, it is then crushed by a rasping machine, so that the cells of the purple sweet potato are fully broken.
[0058] (3) Extraction Add acid aqueous solution to the crushed purple sweet potato cells for extraction, adjust the pH of the extraction solution to 2.0, the ratio of solid to liquid is 1:3, and extract for 1 hour to obtain the extraction solution.
[0059] (4) centrifugal
[0060] The extract is centrifuged with a horizontal spiral centrifuge, and solid-liquid separation is performed to obtain an ...
Embodiment 2
[0080] Such as figure 2 As shown, the present embodiment adopts the commercially available 100g purple sweet potato anthocyanin crude product deep purification example, comprising the following steps:
[0081] (1) Preparation of anthocyanin solution
[0082] Dissolve 100 g of commercially available purple potato anthocyanins in pure water to prepare an anthocyanin solution with a solid content concentration of 20%.
[0083] (2) Yeast activation
[0084] Dried Saccharomyces cerevisiae was added to 5% sugar water at a dosage of 5%, rehydrated at 38°C for 15min, and then rapidly cooled to 30°C for 1h activation.
[0085] (3) fermentation
[0086] Adjust the pH value of the anthocyanin solution to 4.5, control the temperature at 60°C, add glucoamylase for enzymolysis, the amount of glucoamylase added is 15u / ml solution, after 2 hours of enzymolysis, adjust the temperature of the anthocyanin solution to 30°C, add The activated Saccharomyces cerevisiae was subjected to aerobic ...
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