A process method for concentrating soybean peptides by using tandem membranes

A process method and technology of soybean peptide, which is applied in the field of concentrating soybean peptide with serial membranes, can solve the problems of microbial bad smell, product quality impact, bitter and salty taste, shorten the production cycle, improve efficiency, and remove the peculiar smell of soybean peptide Effect

Active Publication Date: 2021-05-14
NUOLI RUYI ANYANG BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main problems of soybean peptide products on the market today are the large molecular weight and heavy bitter and salty taste.
[0004] At present, soybean peptide manufacturers at home and abroad use evaporation concentration or single-membrane double-membrane concentration soybean peptide liquid. Such methods generally require a lot of energy or a long time to concentrate soybean peptide liquid to spray concentration, resulting in long retention time of peptide liquid and microorganisms. It is easy to reproduce and produce some bad smells. At the same time, due to the long production cycle found in the production process, it will affect the quality of the product in many aspects

Method used

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  • A process method for concentrating soybean peptides by using tandem membranes
  • A process method for concentrating soybean peptides by using tandem membranes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:

[0020] (1) Using commercially available soybean protein isolate as raw material, add saturated NaOH solution at 20°C, adjust the pH to 9, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkaline solution;

[0021] (2) Keep the temperature of the above soybean protein isolate alkaline solution at 40°C, add Alcalase protease according to 10‰ of the weight of soybean protein isolate, stir for 2 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3500r / min centrifuged for 15min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 20BX, and the pH value is 6;

[0022] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorization ...

Embodiment 2

[0026] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:

[0027] (1) Using commercially available soybean protein isolate as raw material, add a saturated NaOH solution at 45°C, adjust the pH to 5, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkali solution;

[0028] (2) Keep the temperature of the above soybean protein isolate alkali solution at 70°C, add Alcalase protease according to 1‰ of the weight of soybean protein isolate, stir for 4 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3000r / min centrifugation for 20min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 5BX, and the pH value is 11;

[0029] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorization ...

Embodiment 3

[0033] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:

[0034] (1) Using commercially available soybean protein isolate as raw material, add saturated NaOH solution at 25°C, adjust the pH to 8, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkaline solution;

[0035] (2) Keep the temperature of the above soybean protein isolate alkaline solution at 50°C, add Alcalase protease according to 5‰ of the weight of soybean protein isolate, stir for 3 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3200r / min centrifugation for 18min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 10BX, and the pH value is 8;

[0036] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorizatio...

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Abstract

The invention discloses a process method for concentrating soybean peptides by adopting serial membranes. Firstly, an alkali solution of soybean protein isolate is prepared, and Alcalase protease is added to the alkali solution of soybean protein isolate, and the enzyme is deactivated after enzymatic hydrolysis, and the supernatant obtained by centrifugation is crude soybean Peptide liquid; the crude soybean peptide liquid is subjected to total reflux nanofiltration operation at room temperature, and the adsorption and deodorization of activated carbon is carried out at the same time as the nanofiltration operation. The nanofiltration operation uses 6 nanofiltration membranes in series, and the molecular weight cut-off is 150Da~ 2000Da, pressure 0.2MPa ~ 1.0MPa, treatment for 5 ~ 20min to remove inorganic salt ions with a relative molecular mass ≤ 150Da, collect and filter out the soybean peptide mixture; spray dry to obtain soybean peptide. The present invention adopts six nanofiltration membranes to process in series to increase the dry matter content of the material, reduce the cost and shorten the production cycle; at the same time, it performs adsorption, deodorization and debittering of activated carbon, completely removes the peculiar smell of soybean peptide and keeps the soybean peptide mixture clear and transparent.

Description

technical field [0001] The invention belongs to the technical field of soybean protein deep processing, and in particular relates to a process method for concentrating soybean peptides by adopting serial membranes. Background technique [0002] Soybean peptide, also known as soybean peptide, is an enzymatic hydrolysis product of soybean protein, usually a peptide mixture composed of 3-6 amino acids, and its molecular weight is mainly concentrated below 1000. As an excellent health food, it not only has the effects of lowering blood fat, easy digestion, easy absorption, promoting energy metabolism and anti-obesity, but also can eliminate fatigue, enhance muscle stretching and anti-allergy. The product integrates nutritional characteristics, health characteristics and processing performance, has multiple regulating functions on the body, and is widely used in the production of food and health products. [0003] The main problems of soybean peptide products on the market today...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 刘新旗付慧彦张海萍王华
Owner NUOLI RUYI ANYANG BIOLOGICAL SCI & TECH
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