Gastroenteric tumor targeted magnetic adriamycin albumin nanoparticles manufactured through pressurization and vibration packing method
An albumin nanoparticle and tumor-targeting technology, which is applied in the field of gastrointestinal tumor-targeted magnetic doxorubicin albumin nanoparticles produced by the pressurized vibration encapsulation method, can solve the problems that anti-tumor drugs cannot be completely replaced
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[0016] 1. Preparation of Nanomagnetic Materials
[0017] Add a certain amount of concentrated hydrochloric acid to the magnetic powder Fe3O4, and stir thoroughly at room temperature for 30 min to completely dissolve it to form a brown-yellow transparent solution. Then add 18% NH4OH, stir while adding, and black precipitate can be seen, adjust the pH value to neutral, and let it stand for 72 h. Discard the supernatant and make a mud paste nano-magnetic substance. Take a certain amount of magnetic substance paste, weigh it, then dry it, weigh it again, and calculate the weight percentage concentration of the magnetic substance.
[0018] 2. Preparation of magnetic doxorubicin albumin nanoparticles
[0019] l ml 2 5% Add 20 mg of purified doxorubicin from the protein, then add magnetic substance paste equivalent to 72 mg of dry weight, and stir thoroughly. Add the stirred mixture to 30 ml cottonseed oil. Phacoemulsify lmin at 4 °C. Take 100ml of cottonseed oil and heat it at ...
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