A method for simultaneous detection of four types of ethanol non-oxidative metabolites in human whole blood

A non-oxidative and metabolite technology, applied in the field of forensic identification and analytical chemistry, can solve the problems of large differences in physical and chemical properties of the four types of non-oxidative metabolism, and achieve the effect of easy operation and promotion, large differences in properties, and high difficulty

Active Publication Date: 2020-09-01
FUDAN UNIV
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Problems solved by technology

[0005] Due to the great difference in the physical and chemical properties of the four types of non-oxidative metabolites of ethanol, the analytical methods reported so far are only aimed at the detection of one or two types of metabolites

Method used

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  • A method for simultaneous detection of four types of ethanol non-oxidative metabolites in human whole blood
  • A method for simultaneous detection of four types of ethanol non-oxidative metabolites in human whole blood
  • A method for simultaneous detection of four types of ethanol non-oxidative metabolites in human whole blood

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Embodiment 1

[0030] Example 1 Detection of 4 types of ethanol non-oxidative metabolites in human whole blood

[0031] The chromatographic conditions are as follows:

[0032] Chromatographic column: Thermo Hypersil Gold C18 column (2.1mm×100mm, 1.9μm); column temperature: 47°C

[0033] Mobile phase: 5% acetonitrile-water (phase A); 90% methanol-water (0.1% formic acid) (phase B); methanol (0.1% formic acid) (phase C); isopropanol (5mM ammonium acetate) (phase D ) gradient elution (as shown in Table 3), flow rate 0.2mL / min.

[0034] Injection volume: 5μL

[0035] Table 3 Gradient elution conditions

[0036]

[0037] The mass spectrometry conditions are as follows:

[0038] ESI; spray voltage: 3.6kV(+) / 2.8kV(-); sheath gas: 35Arb; auxiliary gas: 10Arb; ion transfer tube temperature: 350°C; desolvation temperature: 300°C.

[0039] Scanning method: multiple reaction monitoring (MRM)

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Abstract

The invention belongs to the technical field of judicial expertise and analytic chemistry, and relates to a method for detecting ethyl alcohol non-oxide metabolins in human whole blood, in particularto a method for simultaneously detecting 4 species of ethyl alcohol non-oxide metabolins (10 kinds in total) in the human whole blood. The method for simultaneously, qualitatively and quantitatively analyzing 4 species of ethyl alcohol non-oxide metabolins with big nature differences is built, has high sensitivity, high specificity and a wide linearity range, can be used for determining the natures and the quantity of the 4 species of ethyl alcohol non-oxide metabolins (10 kinds in total) in 50mu L of human whole blood few examined material, is simple and fast to operate and less in extractionsolvent dosage, can meet the requirements on judicial expertise task urgency and high detection time demand, and can be popularized and applied on a large scale.

Description

technical field [0001] The invention belongs to the technical field of forensic identification and analytical chemistry, and relates to a method for detecting ethanol non-oxidative metabolites in human whole blood, in particular to a method for simultaneously measuring 4 types of non-oxidative metabolites of ethanol in human blood. Background technique [0002] Ethanol, commonly known as alcohol, is a major substance of abuse worldwide. In forensic practice, in traffic accidents, suspected poisoning suicides, homicide cases, especially in cases involving death, it is necessary to analyze and explain the blood alcohol concentration (BloodAlcohol Concentration, BAC), and the test results are often used to judge Key evidence of party liability. [0003] Currently, the "gold standard" for BAC detection is the determination of ethanol by headspace gas chromatography. However, this method cannot deal with the following two situations. (1) Situation 1: Since drunk driving was sen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 饶渝兰林泽彬张馨予李椒纶黄志斌沈忆文赵子琴
Owner FUDAN UNIV
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