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Method for extracting quercetin and isoquercitrin from sticktight

A technology of isoquercitrin and ghost needle grass, which is applied in the field of isoquercitrin and quercetin extraction, can solve the problems of low solubility, harsh equipment requirements, and low selectivity

Inactive Publication Date: 2018-02-02
桂林纽泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so the supercritical C0 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

Method used

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  • Method for extracting quercetin and isoquercitrin from sticktight
  • Method for extracting quercetin and isoquercitrin from sticktight
  • Method for extracting quercetin and isoquercitrin from sticktight

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The method for extracting quercetin and isoquercitrin from ghost needle grass, specifically comprises the following steps:

[0026] 1) Clean the ghost needle grass, dry it, crush it with a pulverizer, and pass it through a 20-mesh sieve;

[0027] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 170-200°C, pressure 8-10Mpa, extraction time 0.5h, extract the ghost needle grass, after the extraction is completed, release the pressure, and collect at 170-185°C and 185-200°C under 8-10Mpa to obtain two parts of the extract , take the liquid and flow into the collection tank to cool to room temperature;

[0028] 3) Purification: Concentrate the extracts obtained in step 2) respectively, control the temperature to ≤50°C, control t...

Embodiment 2

[0030] The method for extracting quercetin and isoquercitrin from ghost needle grass, specifically comprises the following steps:

[0031] 1) Clean the ghost needle grass, dry it, crush it with a pulverizer, and pass it through a 40-mesh sieve;

[0032]2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 170-200°C, pressure 8-10Mpa, extraction time 1.0h, extract the ghost needle grass, after the extraction is completed, release the pressure, and collect at 170-185°C and 185-200°C respectively under 8-10Mpa to obtain two parts of the extract , take the liquid and flow into the collection tank to cool to room temperature;

[0033] 3) Purification: Concentrate the extract obtained in step 2) respectively, control the temperature to ≤50°C...

Embodiment 3

[0035] The method for extracting quercetin and isoquercitrin from ghost needle grass, specifically comprises the following steps:

[0036] 1) Clean the ghost needle grass, dry it, crush it with a pulverizer, and pass through an 80-mesh sieve;

[0037] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 170-200°C, pressure 8-10Mpa, extraction time 1.0h, extract the ghost needle grass, after the extraction is completed, release the pressure, and collect at 170-185°C and 185-200°C respectively under 8-10Mpa to obtain two parts of the extract , take the liquid and flow into the collection tank to cool to room temperature;

[0038] 3) Purification: Concentrate the extract obtained in step 2) respectively, control the temperature to ≤50°C,...

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Abstract

The invention discloses a method for extracting quercetin and isoquercitrin from P. punctatus, comprising the following steps: 1) cleaning P. punctatus, drying, pulverizing with a pulverizer, and sieving; 2) Subcritical water extraction: put the treated raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle, and extract at a temperature of 170-200°C and a pressure of 8 ‑10Mpa, the extraction time is 0.5‑1.0h, extract the ghost needle grass, the extraction is completed, the pressure is released, and two parts of the extract are collected at 170‑185°C and 185‑200°C respectively under 8‑10Mpa, and the liquid flows into the collection Cool in the tank to room temperature; 3) Purification: Concentrate the extract obtained in step 2), perform column chromatography through a macroporous resin, and elute with 50% v / v ethanol to obtain an eluate, concentrate, and dry to obtain the finished product .

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting quercetin and isoquercitrin from ghost needle grass. Background technique [0002] Quercetin (Quercetin), also known as: quercetin, quercetin, molecular formula C 15 h 10 o 7 , molecular weight 302.23, physical and chemical properties: yellow needle crystal, soluble in glacial acetic acid, yellow in alkaline aqueous solution, almost insoluble in water, ethanol solution tastes very bitter. Quercetin is a flavonoid compound, mostly in the form of glycosides, which can be obtained by acid hydrolysis. Quercetin can be used as a drug, which has good expectorant and cough-relieving effects, and has a certain anti-asthma effect. In addition, it has the effects of lowering blood pressure, enhancing capillary resistance, reducing capillary fragility, lowering blood lipids, expanding coronary arteries, and increasing coronary blood flow. For...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40C07H17/07C07H1/08
CPCC07D311/30C07D311/40C07H1/08C07H17/07
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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