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Matrix, preparation method thereof and biological sample testing method

A biological sample and matrix technology, applied in the field of mass spectrometry, can solve problems such as difficult to meet the detection of small molecules in complex biological systems, and achieve the effects of fast sample detection in the whole process, good protein resistance, and convenient operation.

Active Publication Date: 2018-01-30
浙江亿纳谱生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in real biological samples, such as serum, cerebrospinal fluid, and breast milk, due to the existence of a large amount of proteins and salts and other substances, there are different pH values, presenting a very complex system. The above-mentioned matrix is ​​still difficult to meet the needs of small molecules in complex biological systems. detection

Method used

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  • Matrix, preparation method thereof and biological sample testing method
  • Matrix, preparation method thereof and biological sample testing method
  • Matrix, preparation method thereof and biological sample testing method

Examples

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preparation example Construction

[0061] Preparation methods include:

[0062] Step S101, providing nucleosomes.

[0063] The core shell is formed by covering a silicon dioxide shell with a ferric oxide core. That is, the silicon dioxide as the shell layer is coated outside the ferric oxide as the core layer.

[0064] As an alternative example, nucleocapsids can be fabricated in the following manner.

[0065] First, the iron source (such as FeCl 3 ·6H 2 O as) is dissolved in ethylene glycol for solvothermal reaction to obtain monodisperse Fe 3 o 4 particles. Usually, Fe 3 o 4 Particles are nanoscale spherical structures. Solvothermal reaction conditions: 200°C, 8-72h.

[0066] Second, the obtained Fe 3 o 4 The particles are dispersed in a mixed solvent of water and ethanol, and ammonia water is used to catalyze the hydrolysis and polycondensation of tetraethyl orthosilicate to nucleate, and react at room temperature for 2 to 8 hours to obtain 3 o 4 A silica layer forms on the surface of the nanop...

Embodiment 1

[0092] This embodiment provides a matrix, which includes aggregates of spherical particles with a core-shell structure.

[0093] The spherical particles with core-shell structure in the aggregate of spherical particles with core-shell structure are nanoparticles with core-shell structure of iron oxide / silicon dioxide / silver-platinum alloy.

[0094] The preparation method of matrix comprises the following steps:

[0095] Step 1: FeCl 3 · 6 h 2 O is used as the iron source, modified by adding trisodium citrate and sodium acetate at the same time, thermally reacting with ethylene glycol as the solvent, and reacting for 8h to 72h at 200°C to obtain monodisperse Fe 3 o 4 nanoparticles;

[0096] Step 2: the Fe obtained in step 1 3 o 4 Nanoparticles are dispersed in a mixed solvent of water and ethanol, and ammonia water is used to catalyze the hydrolysis and polycondensation of tetraethyl orthosilicate to nucleate, and react at room temperature for 2h to 8h to obtain 3 o 4 ...

Embodiment 2

[0109] In this example, the iron oxide / silicon dioxide / silver-platinum alloy core-shell structure nano-matrix was used to perform matrix-assisted laser desorption ionization mass spectrometry detection on small molecule standards. Small molecule standards include glucose standards and lysine standards.

[0110] The detection steps are as follows:

[0111] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. DataExplorer was used to observe, process, and analyze data, and only mass spectrometer signals with a signal-to-noise ratio greater than 10 were used for analysis.

[0112] (2) Preparation of iron oxide / silic...

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Abstract

The invention discloses a matrix, a preparation method thereof and a biological sample testing method and belongs to the field of mass spectrometry. The matrix can be used for matrix-assisted laser desorption ionization mass spectrometry. Aggregate of a particulate matter is contained in the matrix and has monodispersity, the particulate matter is of a spherical core-shell structure with a coarsesurface, and particle size is smaller than 1um. The particulate matter comprises a core layer, a first shell layer and a second shell layer coated by the first shell layer, the core layer is ferroferric oxide, the first shell layer is silicon dioxide, and the second shell layer is a silver-platinum compound. The matrix is easy to obtain and highly resistant to salt, and can simplify the biologicalsample pretreatment process and improve detection on desorption ionization of micromolecules when being used for mass spectrometry, so that high sensitivity can be realized within a wide detection range, and biological samples needed for consumption are few.

Description

technical field [0001] The invention relates to the field of mass spectrometry, in particular to a substrate, a preparation method thereof, and a biological sample detection method. Background technique [0002] Mass spectrometry (MS) is an analysis method that converts the molecules to be measured into gas-phase ions through a specific ion source, and then uses electric and magnetic fields to separate them according to different mass-to-charge ratios (mass-to-charge ratios) for detection. The detection of biomolecules by mass spectrometry has the advantages of high specificity, high precision, and short analysis time, so it is widely used. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a new type of soft ionization mass spectrometry developed from laser desorption (LD) in recent years. Matrix-assisted laser desorption ionization mass spectrometry introduces a matrix (Matrix), and the matrix plays the role of absorbing, transmitting laser energy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
Inventor 万馨泽刘彬
Owner 浙江亿纳谱生命科技有限公司
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