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Preparation method of porcine frozen semen diluent

A technology of freezing semen and diluent, applied in the field of swine frozen semen, which can solve the problems of sperm motility, acrosome integrity rate and plasma membrane integrity rate, low conception rate and litter rate of sows, and lower utilization rate of high-quality boars and other problems, to achieve the effect of no immune response, maintenance of fertilization ability, and easy operation

Inactive Publication Date: 2018-01-26
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the sperm motility, acrosome integrity rate, and plasma membrane integrity rate of pig sperm after freezing are not ideal, resulting in the conception rate and litter rate of sows artificially inseminated with frozen semen in commercial pig production. It is relatively low and cannot be widely used, which seriously reduces the utilization rate of high-quality boars, the cost of feeding cannot be reduced, and the pollution of the ecological environment has not been alleviated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing a pig frozen semen dilution, the dilution comprises the following weight components and the steps of configuring the dilution solution: double distilled water 100ml, tris 0.45g, citric acid 0.71g, monosaccharide 5.0g, penicillin 0 Ten thousand u, streptomycin 0.20g, inositol compound 0.29g, glycerin 6.7g, fresh egg yolk 25ml;

[0022] S1: Take the material, take 100ml of double distilled water and put it in a beaker, add tris, citric acid, simple sugar, penicillin, streptomycin, inositol compounds and glycerin in the above ratio to the beaker, and then evenly Stir for 20 minutes to obtain a solution;

[0023] The temperature of double distilled water is 32°C, the monosaccharide is a mixture of any two of glucose, fructose and lactose, and the inositol compound is 1,3-cyclohexanediol or 1,4-cyclohexanediol or 1 , 3,5-cyclohexanetriol or a mixture of the three;

[0024] The inositol compound in the present invention can effectively reduce the form...

Embodiment 2

[0033] A preparation method of pig frozen semen dilution liquid, the dilution liquid comprises the following weight components and the configuration steps of the dilution solution: double distilled water 100ml, tris 0.75g, citric acid 0.79g, monosaccharide 6.40g, penicillin 50g Ten thousand u, streptomycin 0.3g, inositol compound 4.84g, glycerin 8.56g, fresh egg yolk 30ml;

[0034] S1: Take the material, take 100ml of double distilled water and put it in a beaker, add tris, citric acid, simple sugar, penicillin, streptomycin, inositol compounds and glycerin in the above ratio to the beaker, and then evenly Stir for 25 minutes to obtain a solution;

[0035] The temperature of double distilled water is 35°C, the monosaccharide is a mixture of glucose, fructose and lactose, and the inositol compound is 1,3-cyclohexanediol or 1,4-cyclohexanediol or 1 , 3,5-cyclohexanetriol or a mixture of the three;

[0036] The inositol compound in the present invention can effectively reduce t...

Embodiment 3

[0045] A method for preparing a pig frozen semen dilution, the dilution comprising the following weight components and the steps of configuring the dilution solution: double distilled water 100ml, tris 0.90g, citric acid 0.86g, monosaccharide 8.10g, penicillin 100g Wanu, streptomycin 0.5g, inositol compound 6.97g, glycerin 9.5g, fresh egg yolk 33ml;

[0046] S1: Take the material, take 100ml of double distilled water and put it in a beaker, add tris, citric acid, simple sugar, penicillin, streptomycin, inositol compounds and glycerin in the above ratio to the beaker, and then evenly Stir for 30 minutes to obtain a solution;

[0047] The temperature of double distilled water is 38°C, the monosaccharide is a mixture of any two of glucose, fructose and lactose, and the inositol compound is 1,3-cyclohexanediol or 1,4-cyclohexanediol or 1 , 3,5-cyclohexanetriol or a mixture of the three;

[0048] The inositol compound in the present invention can effectively reduce the formation ...

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Abstract

The invention discloses a preparation method of a porcine frozen semen diluent. The diluents comprises the following components by weight, and the preparation steps of the diluent includes: S1: material taking, S2: pH value adjustment, S3: fresh egg yolk adding, S4: centrifugation, S5: standing, and S6: filtration. The inositol compound employed in the invention can effectively reduce the formation of ice crystals formed in a semen freezing process, reduce the mechanical injury of ice crystals on sperms, and finally improve the motility rate, vitality, acrosome integrity, plasmolemma integrityand other technical indexes of porcine frozen semen, wherein penicillin and streptomycin mainly play the role of sterilization and microbial growth; monosaccharide is used as the main energy substance; citric acid and trihytdroxymethyl aminomethane interact to mainly serve as a buffer solution for decreasing sperm motility, thereby prolonging sperm lifetime and maintaining the fertilizing capability.

Description

technical field [0001] The invention relates to the technical field of porcine frozen semen, in particular to a preparation method of porcine frozen semen diluent. Background technique [0002] With the improvement of people's living standards, scientific breeding will be of great help to the breeding of livestock. The research on the cryopreservation of livestock semen has been carried out for more than 50 years, but the vitality of the semen after thawing is only about 30%-50%. Artificial insemination The non-return rate and litter rate were significantly lower than those of fresh and cryogenically refrigerated semen (self-bred and semen stored at room temperature). Pig breeding plays an essential role in our livestock breeding, but during the freezing process of pig semen, the mechanical damage caused by ice crystals formed inside and outside the cells directly leads to the decline of sperm motility after freezing. How to reduce the production of ice crystals, Reducing t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 李兆华于永生张志彬钱芙蓉高见红李娜刘庆雨张琪王俊恒孟庆祝柳海星张树敏金鑫
Owner JILIN ACAD OF AGRI SCI
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