Liquid chromatographic analysis method for simultaneously quantifying glycine and iminodiacetic acid from diethanol amine dehydrogenation product

Abstract

The invention discloses a liquid chromatographic analysis method for simultaneously quantifying glycine and iminodiacetic acid from a diethanol amine dehydrogenation product. The method comprises thefollowing steps: taking toluene sulfonyl chloride acetonitrile as a derivating agent; carrying out derivative reaction on the toluene sulfonyl chloride acetonitrile and amino acid in the diethanol amine dehydrogenation product, and then carrying out liquid chromatographic analysis; and separately drawing standard curves for the concentration of the iminodiacetic acid and the glycine by taking thepeak area of an iminodiacetic acid derivative and the peak area of a glycine derivative. The peak area of an iminodiacetic acid (IDA) standard substance and the concentration of a standard substance have linear dependence in the range of 0.5 g / L-2.500 g / L, the peak area of a glycine (Gly) standard substance and the concentration of a standard substances have linear dependence in the range of 0.01g / L-0.15 g / L, the coefficients of association are greater than 0.9990, and the relative standard deviation is smaller than 1.3%. The analysis result is accurate, analysis steps are simple and convenient, analysis sensitivity is high, the method can be used for quantitative analysis on the iminodiacetic acid as a product and the glycine as a by-product in diethanol amine dehydrogenation reaction, can also be used for liquid chromatography-mass spectrometry qualitative analysis of a product, and has important guidance significance on scientific research and industrial production on preparation of the iminodiacetic acid by diethanol amine catalytic dehydrogenation.

Description

Technical field [0001] The invention relates to a quantitative analysis method capable of simultaneously determining glycine and iminodiacetic acid in diethanolamine dehydrogenation products. Background technique [0002] Iminodiacetic acid (IDA) is an important chemical raw material and an important chemical intermediate with a wide range of uses. It can be used as an intermediate for pesticide oxalylic acid. It can also be used in the electroplating industry, electronics, dyes and water treatment. And many other fields. [0003] At present, the commonly used analytical methods of iminodiacetic acid are: potentiometric titration, ion chromatography, ultraviolet spectrophotometry, gas chromatography pre-column derivatization and reversed-phase high performance liquid chromatography. [0004] (1) Potentiometric titration: Li Yanni. Study on copper zirconium catalyst for the oxidative dehydrogenation of diethanolamine to iminodiacetate. [D]. Zhejiang: Zhejiang Normal University, 2011:...

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Problems solved by technology

Such reagents have disadvantages such as high derivatization reaction temperature, harsh derivatization conditions, and toxic derivatization reagents.

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