Reverse micelle system used for extracting proteins, and preparation method and application thereof

A technology of reverse micelles and proteins, applied in the field of bioengineering, to achieve good biodegradability, biocompatibility, and high extraction rate

Inactive Publication Date: 2018-01-19
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant patents and Chinese and foreign literature reports on the method of extracting soybean protein using starch laurate reverse micelles system

Method used

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  • Reverse micelle system used for extracting proteins, and preparation method and application thereof
  • Reverse micelle system used for extracting proteins, and preparation method and application thereof
  • Reverse micelle system used for extracting proteins, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The starch laurate reverse micelles system for extracting protein is prepared according to the following method:

[0034] 1. Synthesis of starch laurate ester

[0035] (1) Add 1 mol / L hydrochloric acid to cornstarch to keep the pH value of the solution at 3-5, stir and react at 60°C for 1-4h, filter, and wash the filter cake with distilled water until the pH is around 7 to obtain an average molecular weight of 20000-40000 degraded corn starch;

[0036] (2) Take 6g of degraded cornstarch in a three-necked flask, add 0.3g LiCl, fill the reaction system with nitrogen to replace the air in the system to achieve an anhydrous environment, add 50mL N-N dimethylacetamide, heat to 80°C, and fully Stir for 30 minutes, add pyridine (0.019-0.111moL) and lauroyl chloride (0.019-0.111moL) in different proportions to the reaction system, react for 30 minutes, cool to room temperature, add appropriate amount of ethanol, precipitate, suction filter, deionized water, anhydrous After wa...

Embodiment 2

[0046] Starch laurate reverse micellar system for extraction of soybean protein:

[0047] For the reverse micelle system (reverse micelle system 3) formed by the starch laurate ester with a lauroyl substitution degree of 1.93 prepared above, add distilled water to configure a reverse micelle system with a concentration of 1.0-4.0 mg / mL. Each system Add 0.015g of soybean protein powder to the mixture, shake at 35°C and 100r / min for 30min, then centrifuge at 8000r / min for 20min, the extraction system will be divided into two layers, the upper layer is the reverse micellar system for extracting protein, and the lower layer is the residue, The residue was removed by filtration, and the supernatant was taken to measure the absorbance A at 280 nm with a UV-Vis spectrophotometer. Using bovine serum albumin as a standard, make a standard curve, and its linear regression equation is: A=0.666C-0.0025, R 2 =0.9999; where A is the absorbance, and C is the protein concentration (mg / mL). ...

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Abstract

The invention provides a reverse micelle system used for extracting proteins, and a preparation method and an application thereof. The reverse micelle system is composed of hydrophobic starch and a nonpolar organic solvent, wherein substitution degree of the hydrophobic starch is 0.5-3; the nonpolar organic solvent includes isooctane and n-octanol according to the volume ratio of 1:1-9:1; the mass-volume ratio (g:mL) of the hydrophobic starch to the nonpolar organic solvent is 0.001:1-0.01:1. A surfactant for preparing a reverse micelle solution is a starch-based product, which has excellent biodegradability and biocompatibility. The reverse micelle system has higher extraction ratio to macromolecular proteins than other reverse micelle systems.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a reverse micelle system, in particular to a reverse micelle system for extracting protein and its preparation method and application. Background technique [0002] Reverse micelles are based on dipole-dipole, electrostatic force, hydrophobic, etc. When the concentration of amphiphilic substances in non-polar organic solvents exceeds the critical aggregation concentration, reverse aggregates will be formed in the organic phase , such as reverse micelles and other forms. The non-polar groups of the reverse micelles face outward, and the interior is a hydrophilic inner core, which can solubilize biologically active components such as proteins. Due to the shielding protection of reverse micelles, these substances can be kept from direct contact with organic solvents to protect the activity of biological substances, thereby realizing the dissolution and separation of certain bio...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 汪源浩徐成张红奎谭凤芝李沅曹亚峰
Owner DALIAN POLYTECHNIC UNIVERSITY
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