Method for producing tyrosol and hydroxytyrosol through heterologous metabolic pathways

Abstract

The invention discloses a method for producing tyrosol and hydroxytyrosol through heterologous metabolic pathways, wherein metabolic pathways for producing hydroxytyrosol are introduced into hosts forproducing the hydroxytyrosol; more specifically, the method comprises the following steps: producing tyrosol by efficiently expressing aminotransferase, ketoacid decarboxylase and alcohol dehydrogenase in the hosts, then introducing 4-hydroxyphenylacetate hydroxylase to produce the hydroxytyrosol. Genes coded by the enzymes are constructed on an expression plasmid or integrated to a genome. Through the method, production hosts capable of producing the hydroxytyrosol by using glucose, glycerinum and tyrosine can be prepared by introducing the genes coded by the enzymes to the hosts; meanwhile,the invention also discloses a biphasic cultivation method, so that the production of the hydroxytyrosol is more efficient.

Description

technical field [0001] The present invention relates to the field of biotechnology, more precisely, the present invention relates to heterologous biosynthesis of tyrosol, the metabolic pathway of hydroxytyrosol and the method of biphasic culture, by enhancing the expression of upstream shikimate pathway enzymes, selecting the source from bacteria, The exogenous enzyme modified by fungi or protein engineering uses original or modified bacteria, fungi, plant cells or animal cells as host cells, and finally improves the production of tyrosol and hydroxytyrosol. Background technique [0002] Tyrosol is one of the main active compounds in Rhodiola, which has significant effects in anti-hypoxia, anti-fatigue, anti-radiation, anti-cancer, anti-aging, and regulation of body immunity. It is mainly used as a compound in the synthesis of medicine and spices. Intermediates, such as synthetic cardiovascular drugs Metoprolol, Betasolol, etc. Hydroxytyrosol (HT) is a natural phenolic comp...

AI Technical Summary

Problems solved by technology

However, due to the lack of effective production methods, hydroxytyrosol cannot be produced on a large scale

At present, hydroxytyrosol is mainly derived from olive leaf extract, or extracted from olive oil or olive oil factory wastewater, but these processes have low recovery rate, long duration, large environmental pollution, low product purity, and are difficult to separate

However, other methods for synthesizing hydroxytyrosol by chemical transformation or biotransformation also have the disadvantages of low yield, complicated path, and long cycle.

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