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Bacterial type biological adsorbent, and a preparation method and application of adsorbent

A biosorbent, bacterial-type technology, applied in the field of bacterial-type biosorbent and its preparation, can solve the problems of chromate carcinogenicity, mutagenicity, ecological environment threat, etc., and achieves low price, low cost, and increased adsorption efficiency Effect

Inactive Publication Date: 2018-01-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chromate is carcinogenic and mutagenic, and poses a great threat to the ecological environment

Method used

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  • Bacterial type biological adsorbent, and a preparation method and application of adsorbent
  • Bacterial type biological adsorbent, and a preparation method and application of adsorbent
  • Bacterial type biological adsorbent, and a preparation method and application of adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of Pseudomonas aeruginosa bacteria suspension comprises the following steps:

[0043] (1) Activation culture of Pseudomonas aeruginosa: Pick one to two rings of Pseudomonas aeruginosa stored on the plate and put them into sterile NB culture medium for shaking culture for 14.5h (37°C, 150r / min) to obtain cultured bacterial liquid A; get 1-2mL cultured bacterium liquid A (inoculum size 4%) to contain Cr (VI) concentration and be 50mg / L in the aseptic NB culture liquid of shaking culture 14.5 hours, obtain the bacterium liquid B of culture; Take 1- 2mL of the cultured bacteria solution B was shaken in the sterile NB culture solution containing Cr(VI) concentration of 100mg / L for 14.5h (14.5h is the best time for the logarithmic phase of growth) to obtain the cultured bacteria solution C; Take 1-2mL of the cultured bacterial solution C to the sterile NB culture solution containing Cr(VI) concentration of 150mg / L and shake it for 14.5h to obtain the cultured ...

Embodiment 2

[0046] A preparation method of bacterial biosorbent, comprising the following steps:

[0047] (1): the Pseudomonas aeruginosa bacterial suspension prepared in Example 1 and water are mixed with ferric chloride of different amounts respectively, obtain 6 kinds of different mixed solutions respectively (concentration of ferric chloride in 6 kinds of mixed solutions 0, 200, 400, 600, 800, 1000mg / L respectively, and the concentration of Pseudomonas aeruginosa in the 6 kinds of mixtures is 0.5g / L (bacteria dry weight));

[0048] (2) Place the 6 kinds of mixed solutions in a shaker for 24 hours at constant temperature and vibration (cultivation temperature is 37°C, rotation speed is 150rpm), centrifuge and wash (centrifuge at 8000rpm for 5min, wash with deionized water) until the supernatant Clarity, colorless, and transparent, that is, 6 kinds of bacterial biosorbents of Pseudomonas aeruginosa modified with different iron ion concentrations were obtained. The bacteria-type biosorb...

Embodiment 3

[0053] A preparation method of bacterial biosorbent, comprising the following steps:

[0054] (1): the Pseudomonas aeruginosa bacterial suspension prepared in Example 1 and water are mixed with ferric chloride to obtain 5 different mixed solutions respectively (concentration 600mg / L of ferric chloride in 5 mixed solutions, The concentration of Pseudomonas aeruginosa in 5 kinds of mixed solutions is respectively 0,0.25,0.5,1.0,2.0g / L (bacteria dry weight));

[0055] (2) Place the five mixtures in a shaker for 20 hours at constant temperature and shake (the culture temperature is 37°C, the rotation speed is 150rpm), centrifuge and wash (centrifuge at 8000rpm for 5min, wash with deionized water) until the supernatant Clarity, colorless and transparent, namely five kinds of bacterial biosorbents modified by iron ions with different concentrations of Pseudomonas aeruginosa. The bacteria-type biosorbent does not need to be dehydrated and dried, and the bacteria-type biosorbent is p...

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PUM

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Abstract

The invention belongs to the technical field of environmental protection, and discloses a bacterial type biological adsorbent, and a preparation method and application of the adsorbent. The method comprises the following steps: (1) mixing a bacterial suspension of pseudomonas aeruginosa with a modified liquid containing iron ions and water to obtain a mixed solution; and (2) carrying out a constant-temperature oscillation reaction on the mixed solution, and performing centrifugally washing to obtain the bacterial type biological adsorbent. The preparation method provided by the invention is simple, and has low cost and a short operation period; the prepared adsorbent maintains the original reducing capacity for hexavalent chromium; the adsorption efficiency is obviously increased; the adsorption speed is obviously increased; and the adsorbent has very good removal efficiency for hexavalent chromium.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a bacterial biosorbent and its preparation method and application. Background technique [0002] Chromium and its compounds are important industrial raw materials, widely used in electroplating, tanning, printing and dyeing and other industries. Chromium pollution in the environment mainly comes from the discharge of a large amount of chromium-containing wastewater, waste gas, and waste residue in industrial production. Chromate is carcinogenic and mutagenic, and poses a great threat to the ecological environment. The most stable and common forms of chromium in the environment are Cr(VI) and Cr(III), and Cr(VI) is an inhalational highly toxic substance, which is highly oxidizing and easily soluble in water, and its toxicity to organisms Much more than Cr(III). Countries around the world and related organizations have promulgated strict laws and reg...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/30C02F1/28C02F3/34C12N1/20C12R1/385
Inventor 吴平霄喻浪风尹华党志朱能武
Owner SOUTH CHINA UNIV OF TECH
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