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A method for extracting quercetin and isoquercitrin from hypericum ascyron L.

A technology for isoquercitrin and Eclipta chinensis is applied in the field of extracting quercetin and isoquercitrin, and can solve the problems of low solubility, complicated operation, oil loss of aromatic compounds and the like

Inactive Publication Date: 2018-01-09
桂林纽泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so the supercritical C0 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

Method used

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  • A method for extracting quercetin and isoquercitrin from hypericum ascyron L.
  • A method for extracting quercetin and isoquercitrin from hypericum ascyron L.
  • A method for extracting quercetin and isoquercitrin from hypericum ascyron L.

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Effect test

Embodiment 1

[0025] The method for extracting quercetin and isoquercitrin from Eclipta chinensis specifically comprises the following steps:

[0026] 1) Clean the red eclipta, dry, pulverize with a pulverizer, and pass through a 20-mesh sieve;

[0027] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 0.5h, extract the red eclipta, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0028] 3) Purification: Concentrate the extracts obtained in step 2) respectively, control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatograp...

Embodiment 2

[0030] The method for extracting quercetin and isoquercitrin from Eclipta chinensis specifically comprises the following steps:

[0031] 1) Clean the red eclipta, dry, pulverize with a pulverizer, and pass through a 40-mesh sieve;

[0032]2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 170-200°C, pressure 8-10Mpa, extraction time 0.5-1.0h, extract the red eclipta, after the extraction is completed, release the pressure, and collect two parts at 170-185°C and 185-200°C under 8-10Mpa respectively Extraction, take the liquid and flow into the collection tank to cool to room temperature;

[0033] 3) Purification: Concentrate the extract obtained in step 2) respectively, control the temperature to ≤50°C, control the vacuum at -0.08±0....

Embodiment 3

[0035] The method for extracting quercetin and isoquercitrin from Eclipta chinensis specifically comprises the following steps:

[0036] 1) Clean Eclipta chinensis, dry, pulverize with a pulverizer, and pass through an 80-mesh sieve;

[0037] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 170-200°C, pressure 8-10Mpa, extraction time 0.5-1.0h, extract the red eclipta, after the extraction is completed, release the pressure, and collect two parts at 170-185°C and 185-200°C under 8-10Mpa respectively Extraction, take the liquid and flow into the collection tank to cool to room temperature;

[0038] 3) Purification: Concentrate the extract obtained in step 2) respectively, control the temperature to ≤50°C, control the vacuum at -0.0...

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Abstract

A method for extracting quercetin and isoquercitrin from hypericum ascyron L. is disclosed. The method includes 1) a step of cleaning and airing the hypericum ascyron L., crushing the hypericum ascyron L. by utilizing a crusher, and sieving the crushed hypericum ascyron L.; 2) a step of performing subcritical water extraction, namely a step of adding the treated raw material into an extraction kettle, adding hydroxyethyl cellulose the weight of which is 0.001 part of the weight of the raw material into the kettle, injecting deoxygenated deionized water into the kettle, extracting the hypericumascyron L. at 170-200 DEG C under 8-10 MPa for 0.5-1.0 h, after the extraction is finished, performing pressure relief, collecting extraction liquids at 170-185 DEG C and at 185-200 DEG C under 8-10MPa respectively, allowing the extraction liquids to flow into collection tanks, and cooling the extraction liquids to room temperature; and 3) a step of purification, namely a step of separately concentrating the extraction liquids obtained in the step 2), separately performing column chromatography utilizing macroporous resin, performing elution with an ethanol solution having a concentration of50% v / v to obtain eluates, concentrating the eluates, and drying concentration products to obtain finished products.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting quercetin and isoquercitrin from Eclipta chinensis. Background technique [0002] Quercetin (Quercetin), also known as: quercetin, quercetin, molecular formula C 15 h 10 o 7 , molecular weight 302.23, physical and chemical properties: yellow needle crystal, soluble in glacial acetic acid, yellow in alkaline aqueous solution, almost insoluble in water, ethanol solution tastes very bitter. Quercetin is a flavonoid compound, mostly in the form of glycosides, which can be obtained by acid hydrolysis. Quercetin can be used as a drug, which has good expectorant and cough-relieving effects, and has a certain anti-asthma effect. In addition, it has the effects of lowering blood pressure, enhancing capillary resistance, reducing capillary fragility, lowering blood lipids, expanding coronary arteries, and increasing coronary blood flow. For ...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40C07H17/07C07H1/08
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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