FGF19 over-expression human liver cancer cell line and application thereof
A liver cancer cell, overexpression technology, applied in the field of cells, can solve the problems of lack, drinking water pollution, poor prognosis of liver cancer patients, etc., and achieve the effect of stable traits
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Embodiment 1LI
[0046] The preparation of embodiment 1LIXC-501 cell
[0047] A. Preparation of LIXC-501 hepatocellular carcinoma human mouse model
[0048] (1) A series of fresh resection specimens of clinical liver cancer were obtained from Shanghai Dongfang Hospital. The clinical diagnosis was recurrence of liver cancer, and the pathological diagnosis was hepatocellular carcinoma, some of which were clear cell subtype.
[0049] Rinse the above specimen with fresh HBSS buffer (HBSS buffer containing 500 U / mL penicillin G, 500 μg / mL streptomycin sulfate and 1.25 μg / mL amphotericin B), cut into small pieces of 20-50 mg, and subcutaneously Immunodeficient animal SCID mice (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.) were puncture-inoculated; after tumor formation, human-derived mouse models of hepatocellular carcinoma were prepared and named respectively. Tumors continued to be passaged in vivo and cryopreserved.
[0050] (2) Through next-generation sequenci...
Embodiment 2LI
[0059] Biological characteristics and application of embodiment 2LIXC-501 cells
[0060] The invention adopts RPMI1640 culture and purifies LIXC-501 cells by a differential attachment method, so that they can grow long-term in vitro and pass stably. When the cells are passed on for more than 20 generations, the cell traits are gradually stabilized, and relevant biology, genetics, and tissue source identification are carried out until the 30th to 50th generations have the same stable traits. According to experimental observation and verification, LIXC-501 grown in vitro has a typical epithelial-like morphology, the main body is flat irregular polygon, with a round nucleus in the middle, and grows in a covering layer. The cells adhere to the wall and are closely connected with each other. shape move. Genetic studies have confirmed that the aneuploid ratio of the cells is about 50%, and the number and structure of chromosomes are seriously aberrated, which is consistent with the...
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