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Microbial water purifier for improving water quality for Brachymystax lenok and purpose thereof

A technology of microorganisms and water purifiers, used in biological water/sewage treatment, water pollutants, water/sludge/sewage treatment, etc. Water quality and disease prevention effect

Inactive Publication Date: 2018-01-05
TONGHUA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Putting the microbial water purifying agent of the present invention into the snakehead culture pond can achieve the purpose of purifying the water in the culture pond, but it is not suitable for improving the water quality of leptospirosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A preparation method of a microbial water purifier for improving the water quality of leptospirosis, specifically comprising the following steps:

[0017] 1) Preparation of PAD medium: Take 20 parts of potatoes, 1.5 parts of glucose, 2 parts of agar, and 98 parts of water by weight, chop the potatoes, put them in boiling water and boil for 30 minutes, filter, add glucose and agar to the filtrate, and add Autoclave at 121°C for 10 minutes to obtain the PAD medium, which is ready for use. The PAD medium is cheap and easy to prepare, and can provide sufficient carbon and nitrogen sources for the strains, which is conducive to the rapid reproduction of the strains;

[0018] 2) Preparation of culture medium: Take 21 parts of wheat bran, 10 parts of peanut shell powder, 18 parts of soybean meal, 0.6 part of table salt, 0.3 part of sodium dihydrogen phosphate, 0.8 part of magnesium sulfate, 1.1 part of calcium chloride by weight, and promote growth 0.25 parts of agent, add wat...

Embodiment 2

[0024] A preparation method of a microbial water purifier for improving the water quality of leptospirosis, specifically comprising the following steps:

[0025]1) Take 19 parts of potatoes, 1.8 parts of glucose, 1.9 parts of agar, and 97 parts of water by weight, put the potatoes into boiling water and boil for 28 minutes, filter, add glucose and agar to the filtrate, and sterilize to obtain the PAD medium. Standby, the PAD medium is cheap, easy to prepare, and can provide sufficient carbon and nitrogen sources for the strains, which is conducive to the rapid reproduction of the strains;

[0026] 2) By weight, take 19 parts of wheat bran, 11 parts of peanut shell powder, 15 parts of soybean meal, 0.7 parts of table salt, 0.3 parts of sodium dihydrogen phosphate, 0.6 parts of magnesium sulfate, 0.9 parts of calcium chloride, and 0.22 parts of growth-promoting agent. The weight ratio is 1:8, add water and mix evenly, pressurize at a pressure of 2.2MPa and a temperature of 140°C...

Embodiment 3

[0031] A preparation method of a microbial water purifier for improving the water quality of leptospirosis, specifically comprising the following steps:

[0032] 1) Take 23 parts of potatoes, 1 part of glucose, 2.2 parts of agar, and 96 parts of water by weight, put the potatoes into boiling water and cook for 28-33 minutes, filter, add glucose and agar to the filtrate, and sterilize to obtain PAD culture base, spare;

[0033] 2) By weight, take 22 parts of wheat bran, 11 parts of peanut shell powder, 18 parts of soybean meal, 0.6 parts of table salt, 0.2 parts of sodium dihydrogen phosphate, 1.3 parts of magnesium sulfate, 1.1 parts of calcium chloride, and 0.23 parts of growth promoting agent. The weight ratio is 1:8, add water and mix evenly, keep the pressure at 2.2MPa and 150°C for 8 minutes, cool, and set aside, the growth promoter is vitamin enzyme, protease and active polypeptide with a weight ratio of 1:1.5:0.15;

[0034] 3) Inoculate the activated strains into the P...

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PUM

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Abstract

The invention discloses a microbial water purifier for improving water quality for Brachymystax lenok and purpose thereof. A preparation method of the water purifier specifically comprises the following steps: putting potatoes in boiling water to cook, filtering, adding glucose and agar into a filtrate, and sterilizing to obtain a PAD culture medium; taking various ingredients according to formularatio of the culture medium, adding water and uniformly mixing, carrying out pressure maintaining treatment, and cooling to obtain a culture medium; inoculating an activated strain into the PAD culture medium and culturing to obtain a seed solution; and inoculating the seed solution and culturing to obtain the microbial water purifier. By adding 15-25 ML of the water purifier into each cubic meter of aquaculture water, effects of nitrification, denitrification, nitrogen reduction, phosphorus removal, removal of organic matter in water and the like can be realized; eutrophication materials such as nitrogen, phosphorus, etc. in water can be greatly removed; other harmful bacteria in a water body can be effectively inhibited; the effects of preventing diseases and raising survival rate are achieved; and the purpose of purifying a water body is achieved.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a microbial water purifier for improving the water quality of leptospirosis and its application. Background technique [0002] Leptospirosis belongs to Salmoniformes, Salmonidae, and Leptospirosis genus, and is a residual species that moved from north to south during the ice age. It is a kind of cold-water fish. It mainly lives in mountain streams with an altitude of 900-2300m in the Qinling Mountains. Because of this, the meat of leptospirosis is very tender and can be directly cut into fish fillets for use. It has no intermuscular spines, is rich in protein, unsaturated fatty acids, essential amino acids, flavor amino acids and other nutrients, and also has the effect of preventing blood clots. Forming and accelerating the healing effect of wounds enjoys the reputation of "brain gold". It is also a national second-class protected aquatic wild animal and has im...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/30C02F103/20C02F101/16C02F101/30
Inventor 何晓燕张媛媛王日昕
Owner TONGHUA NORMAL UNIV
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