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Method for detecting influence of cigarette smoke on expression quantity of aquaporin 5 cells

A technology of aquaporin and cigarette smoke, which is applied in the field of detection of the influence of cigarette smoke on the expression of aquaporin 5 cells, can solve the problems of inapplicability to large-scale sample detection, and achieve fast, accurate and visual multi-sample detection Effect

Active Publication Date: 2017-12-26
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the effects of chemical substances on aquaporins in medicine are studied on the AQP5 at the whole animal level through the animal model of Sjogren's syndrome, which is not suitable for the detection of large quantities of samples

Method used

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  • Method for detecting influence of cigarette smoke on expression quantity of aquaporin 5 cells
  • Method for detecting influence of cigarette smoke on expression quantity of aquaporin 5 cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for detecting the influence of cigarette smoke on the expression level of aquaporin 5 cells, comprising the steps of:

[0034] Step (1), using a bionic absorption device with patent application number 201520354840.8 for studying mainstream smoke exposure in the oral cavity, using DMEM / F12 cell culture medium to infiltrate the simulated artificial oral mucosa inner wall of the device; select 10 weights A cigarette consistent with the suction resistance is used as the sample to be tested, and after being balanced, it is sucked by a fully automatic rotary smoking machine. After the suction is completed, the liquid in the bottle of the bionic absorption device is taken out to obtain a bionic suction liquid; The equilibrium conditions are: temperature 21°C, humidity 58%, time 48h. The suction frequency of the suction is 1 puff / min, the suction duration is 2 s, and the suction volume is 34.85 mL / puff.

[0035] Step (2), the salivary gland adenoid cystic carcinoma ce...

Embodiment 2

[0042] A method for detecting the influence of cigarette smoke on the expression level of aquaporin 5 cells, comprising the steps of:

[0043] Step (1), using a bionic absorption device with patent application number 201520354840.8 for studying mainstream smoke exposure in the oral cavity, using 5mL DMEM / F12 cell culture medium to infiltrate the simulated artificial oral mucosa inner wall of the device; select 20 The cigarette with the same weight and suction resistance is used as the sample to be tested, and after being balanced, it is sucked by a fully automatic rotary smoking machine. After the suction is completed, the liquid in the bottle of the bionic absorption device is taken out to obtain the bionic suction liquid; The equilibrium conditions are: temperature 23°C, humidity 62%, time 48h. The suction frequency of the suction is 1 puff / min, the suction duration is 2 s, and the suction volume is 35.15 mL / puff.

[0044] Step (2), the salivary gland adenoid cystic carcino...

Embodiment 3

[0052] A method for detecting the influence of cigarette smoke on the expression level of aquaporin 5 cells, comprising the steps of:

[0053] Step (1), using a bionic absorption device with patent application number 201520354840.8 for studying mainstream smoke exposure in the oral cavity, using 10mL DMEM / F12 cell culture medium to infiltrate the simulated artificial oral mucosa inner wall of the device; select 15 The cigarette with the same weight and suction resistance is used as the sample to be tested, and after being balanced, it is sucked by a fully automatic rotary smoking machine. After the suction is completed, the liquid in the bottle of the bionic absorption device is taken out to obtain the bionic suction liquid; The equilibrium conditions are: temperature 22°C, humidity 60%, time 48h. The suction frequency of the suction is 1 puff / min, the suction duration is 2 s, and the suction volume is 35 mL / puff.

[0054] Step (2), the salivary gland adenoid cystic carcinoma...

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Abstract

The invention relates to a method for detecting the influence of cigarette smoke on the expression quantity of aquaporin 5 (AQP-5) cells, and belongs to the technical field of biological application. According to the method, biochemical and cytological methods are used, BMP6 is used to process the cells, and the cellular microenvironment when sjogren syndrome happens is simulated so as to study the influence of cigarette smoke on the expression quantity of AQP-5; compared with animal experiments, the method is simpler and more efficient; an oral cavity biomimetic-simulation device is used for collecting cigarette smoke, which is closer to the actual state of human smoking; meanwhile, a high content system is used and can detect the influence of multiple samples on the expression quantity of the AQP-5 cells in a high flux, the multi-sample detection can be conducted rapidly, accurately and visually, and a novel rapid means is provided for the efficiency evaluation of cigarette smoke.

Description

technical field [0001] The invention belongs to the technical field of biological applications, and in particular relates to a method for detecting the influence of cigarette smoke on the expression level of aquaporin 5 cells. Background technique [0002] Saliva is the main factor to maintain the balance of the oral micro-ecological environment. It has many functions such as moistening the mouth and food, facilitating speaking and swallowing, removing food particles on the taste buds, so as to continuously taste the taste of food, cleaning and protecting the oral cavity. The sensory evaluation of cigarettes shows that some cigarette smoke can cause negative feelings such as dry mouth, dryness, and discomfort. Therefore, the development of cigarette products with the function of reducing dryness and promoting body fluids is of great significance for improving oral comfort and smoking quality of cigarettes. [0003] The aquaporin family (Aquaporins, AQP) is a group of protein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCC12N2503/00G01N33/5023
Inventor 高茜管莹曾婉俐向海英夭建华杨叶昆张承明杨光宇李雪梅
Owner CHINA TOBACCO YUNNAN IND
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