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Method for extracting astaxanthin from haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, applied in the direction of organic chemistry, etc., can solve the inevitable problems of incomplete recovery of organic solvents, long extraction time, complicated process, etc., achieve ideal cutting force and wall breaking efficiency, improve Grinding and wall breaking effect and easy operation steps

Active Publication Date: 2017-12-26
博露(厦门)生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main problem in the extraction is that the wall of Haematococcus pluvialis is thick, and the effect of cell wall breaking is poor, which leads to low extraction rate. In addition, there are problems such as complicated process, high equipment cost, and organic solvent residue.
Chinese invention patent application CN102012363A uses high-pressure homogenization method to break the wall at 80-90MPa and then uses supercritical CO 2 Extraction, the extraction temperature is 60-80°C, and the extraction time is 2-4h. This method has high equipment costs, long extraction time, and high cost, which is not conducive to industrial production
Chinese invention patent application CN106432024A uses an organic solvent and a sand mill to grind and break algae cells, and recovers the organic solvent through liquid phase decompression distillation. The entire extraction process time is shortened, but it is inevitable that the organic solvent cannot be completely recovered, and there is a problem of organic solvent residue, and easy to cause environmental pollution

Method used

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  • Method for extracting astaxanthin from haematococcus pluvialis
  • Method for extracting astaxanthin from haematococcus pluvialis
  • Method for extracting astaxanthin from haematococcus pluvialis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Effect of different solid-liquid ratios of algae powder and extractant oil on the extraction effect of astaxanthin

[0033] The solid-liquid ratio of algae powder and extractant oil affected the concentration of astaxanthin oil and the extraction rate of astaxanthin. The increase of extractant oil can increase the extraction rate of astaxanthin, but it will reduce the concentration of astaxanthin oil, and the reduction of astaxanthin oil concentration will lead to the increase of transportation cost, raw material cost and storage cost of astaxanthin oil for subsequent application. Therefore, it is necessary to increase the concentration of astaxanthin oil as much as possible, but as the amount of algae powder increases, the loss rate of extractant oil increases, and the volume of effective astaxanthin oil decreases, resulting in a decrease in the extraction rate of astaxanthin. Therefore, a Appropriate material-to-liquid ratio.

[0034] Get 6 parts of 10ml ca...

Embodiment 2

[0038] Example 2 The influence of water bath temperature on the extraction effect of astaxanthin

[0039] Accurately weigh 4.0g Haematococcus pluvialis algae powder, add 10ml camellia seed oil and 0.4g glass beads with sizes of 50 mesh and 270 mesh respectively (the mass ratio of the two glass beads with different sizes and specifications is 1:1), set Oscillate on a vortex oscillator for 15 minutes, the oscillation frequency is 2800r / min, put it in a water bath for 15 minutes, and set the temperature of the water bath to 0°C, 15°C, 30°C, 40°C, 50°C, 60°C, 70°C, 80°C, 90 ℃, 100 ℃, shake once every 5 minutes during the water bath, centrifuge at 3000 rpm for 3 minutes after the water bath, take the upper liquid and filter it on the glass fiber membrane to obtain astaxanthin oil. Observe the effect of water bath temperature on the extraction effect of astaxanthin, the results are shown in figure 2 .

[0040] From figure 2It can be seen that as the temperature of the water bat...

Embodiment 3

[0041] Example 3 The influence of water bath time on the extraction effect of astaxanthin

[0042] Accurately weigh 4.0g Haematococcus pluvialis algae powder, add 10ml camellia seed oil and 0.4g glass beads with sizes of 50 mesh and 270 mesh respectively (the mass ratio of the two glass beads with different sizes and specifications is 1:1), set Vibrate on a vortex oscillator for 15 minutes, the oscillation frequency is 2800r / min, put it into a water bath for water bathing, the water bath temperature is 70°C, the water bath time is 0-60min, oscillate once every 5min during the water bath, centrifuge at 3000rpm for 3min after the water bath is over , take the upper liquid and filter it on a glass fiber membrane to obtain astaxanthin oil. Observe the effect of water bath time on the extraction effect of astaxanthin, the results are shown in image 3 .

[0043] From image 3 It can be seen that as the water bath time prolongs, the concentration of astaxanthin oil increases, and...

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Abstract

The invention discloses a method for extracting astaxanthin from haematococcus pluvialis, which includes the following steps: (1) haematococcus pluvialis powder is weighed, added with two types of glass beads with different specifications and extracting agent oil and uniformly mixed; (2) the solution obtained in step (1) is shaken in a vortex shaker and extracted in water bath, so that a suspension is obtained; (3) the suspension obtained in step (2) undergoes solid-liquid separation, so that astaxanthin oil is obtained. With vegetable oil as the extracting agent oil, the method adopts a mechanical and physical extraction method; the two types of glass beads with different specifications as abrasive material accord with the morphological characteristics of haematococcus pluvialis cells, and can effectively increase the effect of breaking the walls of the haematococcus pluvialis cells by grinding in comparison with an abrasive material with single specifications; and the method for extracting astaxanthin from haematococcus pluvialis has the advantages of simple operation steps, no organic solvent consumption, high extraction rate, low cost and easy industrial production.

Description

technical field [0001] The invention belongs to the technical field of biological separation, and in particular relates to a method for extracting astaxanthin from Haematococcus pluvialis. Background technique [0002] Astaxanthin is a red natural carotenoid, which has the function of scavenging oxygen free radicals. Its antioxidant capacity is 550 times that of vitamin E and 10 times that of β-carotene. It is known as a super antioxidant. At present, there are four main sources of astaxanthin: chemical synthesis, extraction from crustaceans, fermentation product of Phaffia rhodozyme and extract of Haematococcus pluvialis. Chemically synthesized astaxanthin contains a mixture of 4 optical isomers. 98% of the astaxanthin produced by the fermentation of Phaffia rhodozyme is the astaxanthin in the R conformation, and only the product accumulated by Haematococcus pluvialis is in the pure S conformation. Has the strongest biological activity. [0003] Haematococcus pluvialis is...

Claims

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Application Information

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IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 陈意民陈瑞凤潘双福
Owner 博露(厦门)生物股份有限公司
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