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Lysinibacillus and application thereof in degrading of zearalenone

A technology of lysine bacillus and zearalenone, which is applied in the field of lysine bacillus and its application in the degradation of zearalenone, which can solve difficult large-scale production, loss of nutrients, and ZEA removal Problems such as unstable effects

Active Publication Date: 2017-12-22
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, scholars at home and abroad have studied a variety of detoxification methods and technologies, which can be divided into three categories according to their technical principles, namely physical, chemical and biological detoxification methods. Among them, irradiation technology, adsorption technology and ozone detoxification have certain advantages. It is an important technology to reduce ZEA pollution in grains, but the traditional detoxification method has an unstable removal effect on ZEA, and the loss of nutrients is large, making it difficult to scale production

Method used

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  • Lysinibacillus and application thereof in degrading of zearalenone
  • Lysinibacillus and application thereof in degrading of zearalenone
  • Lysinibacillus and application thereof in degrading of zearalenone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Isolation and Identification of Bacterial Strains

[0032] 1. Isolation of strains

[0033] (1) In a sterile workbench, take out the intestinal contents of the chicken and mix them evenly, weigh 0.25-0.5g and add them to 10mL LB liquid medium containing 20ppm ZEA at a final concentration for acclimation and cultivation on a shaking table, the shaking table speed is 200r / min, the shaker temperature was 37°C, and the acclimatization time was 96h.

[0034] (2) Take the above-mentioned acclimatized bacterial solution in the aseptic operating table and inoculate it into 10 mL of LB liquid medium containing 20 ppm ZEA in a volume ratio of 1% and inoculate it on a shaking table for acclimation and cultivation. 37°C, the acclimatization time is 96h. Repeat this cycle 2-3 times.

[0035] (3) Dilute the bacterial solution after the last acclimatization culture with sterile physiological saline, then spread it on the LB medium plate, and cultivate it at 37°C for 24 ...

Embodiment 2

[0043] Example 2: Application of Bacillus lysinica ZJ-2016-1 culture solution in degrading zearalenone

[0044] 1. Culture and activation of Bacillus lysinica ZJ-2016-1

[0045] Lysinibacillus macroides ZJ-2016-1 was inoculated in liquid LB medium, cultured by shaking at a temperature of 37°C and a rotation speed of 200r / min (rotation radius: 20mm) for 24h, and the culture products were collected It is the ZJ-2016-1 culture medium, which is used for the subsequent zearalenone degradation experiment.

[0046] 2. Degradation of zearalenone by Bacillus lysinica ZJ-2016-1 culture solution

[0047] (1) Preparation of Zearalenone Solution

[0048] 10 mg of zearalenone standard substance (Sigma-Aldrich, product number Z2125) was dissolved in 50 mL of chromatographically pure methanol to obtain a zearalenone solution with a concentration of 200 ppm.

[0049] (2) Degradation of zearalenone

[0050] Experimental group: Take 1 mL of zearalenone solution with a concentration of 200 pp...

Embodiment 3

[0058] Example 3: Degradation of zearalenone in corn feed by Bacillus lysinica ZJ-2016-1 culture solution

[0059] 1. Degradation of zearalenone in corn feed by Bacillus lysinica ZJ-2016-1 culture solution

[0060] Weigh 450g of corn feed, add zearalenone standard substance (Sigma-Aldrich, product number Z2125) to make the final concentration 10ppm, mix well, divide into 9 parts on average, do three different treatments, each treatment set 3 repetitions, the first one was added with 50mL sterile water (blank group), the second one was added with 50mL sterilized LB culture medium (control group), and the third one was added with a cell concentration of 10 7 cell / mL ZJ-2016-1 culture solution (experimental group), and then placed in a 37°C constant temperature incubator for 48h.

[0061] 2. Detection of zearalenone

[0062] The preparation of PBS buffer solution (0.01mol / L): mix 8g NaCl, 1.44g Na 2 HPO 4 , 0.24g KH 2 PO 4 1. Dissolve 0.2g KCl in 800mL deionized water, adju...

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Abstract

The present invention pertains to the field of microorganisms and feed additives, and discloses a lysinibacillus macroides microorganism capable of degrading zearalenone. The microorganism is obtained from chicken gastrointestinal content by toxin domestication, gradient dilution and separation, and screening. The strain is identified as the lysinibacillus macroides microorganism, can degrade the zearalenone efficiently, and has good application prospects in the development of new biodegradable bacteria agents and biodegradable sterile preparations.

Description

technical field [0001] The invention relates to the field of microorganisms and feed additives, in particular to a strain of lysine bacillus and its application in degrading zearalenone. Background technique [0002] Zearalenone (ZEA) is a non-steroidal mycotoxin with estrogen-like effects, which is mainly synthesized and secreted by Fusarium graminearum, Fusarium pink, Fusarium moniliforme, and Fusarium tritinarum. Every year, ZEA pollution brings significant economic losses to planting and animal husbandry all over the world. Since grain crops such as wheat and corn are easily infected by molds in the field, if the environmental conditions such as humidity and temperature are suitable, molds will grow and reproduce in large numbers and produce various mycotoxins during transportation, processing and storage. In China, this pollution phenomenon is even more serious due to the impact of harvesting, storage and processing techniques. Feed mildew has caused huge losses to ag...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L5/20A23K10/18C12R1/01
CPCA23K10/18A23L5/28C12N1/20C12N1/205C12R2001/01
Inventor 王金全杨凡刘杰吕宗浩陈瀛
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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