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Human adipose-derived mesenchymal stem cell anti-hepatic fibrosis injection and preparation method thereof

An anti-liver fibrosis and stromal cell technology, applied in the field of human adipose-derived mesenchymal stem cell anti-liver fibrosis injection and its preparation, can solve the problems of increasing the risk of clinical treatment of mesenchymal stem cells, and achieve activation and maintenance Mesenchymal stem cell activity, solving the effect of short cell transit time and stable osmotic pressure

Inactive Publication Date: 2017-12-22
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in the process of stem cell infusion, it is very easy to promote cells to aggregate into clusters and form cell plugs, which increases the risk of clinical treatment of mesenchymal stem cells

Method used

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  • Human adipose-derived mesenchymal stem cell anti-hepatic fibrosis injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Preparation of human adipose-derived mesenchymal stem cell preservation solution A 100ml of the present invention

[0045] Under aseptic conditions, take 36.5ml of compound electrolyte injection (trade name: Bomaili A, Shanghai Baite Medical Supplies Co., Ltd., H20000475), and 36.5ml of hydroxyethyl starch 200 / 0.5 sodium chloride for injection Liquid (commodity name: Hess, Beijing Fresenius Kabi Pharmaceutical Co., Ltd., Chinese medicine standard H20030494, specification and model 500ml: 15g hydroxyethyl starch 200 / 0.5 and sodium chloride 4.5g), 4ml human serum albumin injection Liquid (Chengdu Rongsheng Pharmaceutical Co., Ltd., Sinopharm S10940024, 50ml: 10g, 20%),

[0046] 4ml Dazhu Rhodiola injection (Tonghua Yusheng Pharmaceutical Co., Ltd., Zhunzi Z20060361, 5ml), 4ml lentinan injection (Jinling Pharmaceutical Co., Ltd. Fuzhou Meifeng Pharmaceutical Factory, Zhunzi H20030131, 2ml: 1mg), 4ml Astragalus Injection (Zhengda Qingchunbao Pharmaceutical Co., Ltd., Z3...

Embodiment 2

[0055] 1. Preparation of human adipose-derived mesenchymal stem cell preservation solution B 100ml of the present invention

[0056] Same as Example 1, under aseptic conditions, take 43.25ml of compound electrolyte injection, 43.25ml of hydroxyethyl starch 200 / 0.5 sodium chloride injection, 2ml of human serum albumin injection, 2ml of Rhodiola rosea injection Liquid, 2ml Lentinan Injection, 2ml Astragalus Injection, 2ml Xiangdan Injection, 2ml Placenta Polypeptide Injection, 1ml Vitamin C Injection, 0.5ml Dexamethasone Sodium Phosphate Injection, mix well, place at 0-8℃ Store refrigerated.

[0057] 2. Preparation of human adipose-derived mesenchymal stem cell anti-hepatic fibrosis injection B 100ml of the present invention

[0058] Same as the second step in Example 1, add the obtained human adipose-derived mesenchymal stem cells into human adipose-derived mesenchymal stem cell preservation solution B; resuspend the cells in the human adipose-derived mesenchymal stem cell pre...

Embodiment 3

[0060] 1. Preparation of human adipose-derived mesenchymal stem cell preservation solution C 100ml of the present invention

[0061] Same as Example 1, under aseptic conditions, take 31.25ml compound electrolyte injection, 31.25ml hydroxyethyl starch 200 / 0.5 sodium chloride injection, 8ml human serum albumin injection, 5ml Dazhu Rhodiola injection , 5ml Lentinan Injection, 5ml Astragalus Injection, 5ml Xiangdan Injection, 5ml Placenta Polypeptide Injection, 3ml Vitamin C Injection, 1.5ml Dexamethasone Sodium Phosphate Injection, mix well, store in refrigerated storage at 0-8℃ .

[0062] 2. Preparation of human adipose-derived mesenchymal stem cell anti-hepatic fibrosis injection C 100ml of the present invention

[0063] As in Example 1, the obtained human adipose-derived mesenchymal stem cells were added to the human adipose-derived mesenchymal stem cell preservation solution C; the cells were resuspended in the human adipose-derived mesenchymal stem cell preservation solutio...

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Abstract

The invention discloses human adipose-derived mesenchymal stem cell anti-hepatic fibrosis injection. The human adipose-derived mesenchymal stem cell anti-hepatic fibrosis injection is prepared from human adipose-derived mesenchymal stem cell preserving fluid and human adipose-derived mesenchymal stem cells; each millimeter of the human adipose-derived mesenchymal stem cell preserving fluid contains 1*10<5> to 5*10<6> human adipose-derived mesenchymal stem cells; a preparation method of the human adipose-derived mesenchymal stem cell anti-hepatic fibrosis injection comprises the following steps: preparing the human adipose-derived mesenchymal stem cell preserving fluid and pre-cooling for later use; then preparing the human adipose-derived mesenchymal stem cells and adding the human adipose-derived mesenchymal stem cells into the mesenchymal stem cell preserving fluid; adjusting the quantity of the human adipose-derived mesenchymal stem cells added into the preserving fluid to 1*10<5> / ml to 5*10<6> / ml in a resuspension manner. The injection disclosed by the invention has the advantages of remarkable curative effect, high stability, convenience for preservation and transportation and safety in utilization and can bring hope to patients with hepatic fibrosis.

Description

[technical field] [0001] The invention belongs to the technical field of biology, and in particular relates to a human adipose-derived mesenchymal stem cell anti-hepatic fibrosis injection and a preparation method thereof. Background technique] [0002] Adipose tissue contains a type of cells with multi-directional differentiation potential, namely adipose mesenchymal stem cells, referred to as adipose stem cells. Its biological properties are similar to those of bone marrow mesenchymal stem cells, and can differentiate into various cells such as fat, bone, cartilage, liver, muscle, endothelium, hematopoietic, pancreatic islets and nerves. [0003] Adipose-derived mesenchymal stem cells have the following advantages: 1. Adipose tissue is widely distributed in the body and has abundant reserves. Moreover, it is easy to obtain adipose tissue, and liposuction is a conventional operation with mature technology, which has low surgical risk and less pain for patients, which is es...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/08A61K47/46A61K47/36A61K47/42A61K47/22A61K47/28A61K47/02A61P1/16
CPCA61K9/0019A61K9/08A61K35/28A61K47/02A61K47/22A61K47/28A61K47/36A61K47/42A61K47/46
Inventor 张炳强
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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