Tissue-culture rapid propagation method for radix paeoniae alba

Abstract

The invention discloses a tissue-culture rapid propagation method for radix paeoniae alba. The radix paeoniae alba belongs to perennial vegetation and is characterized by large roots, cylindrical shape and black brown or claybank surface; the stem is upright, smooth and glabrous; the leaves alternately grow; the leaves grow on the lower stem; the small blades are narrow and are of oval, ellipse or lanceolate shape; the top is sharp, the base is of a wedge shape and the leaf surface is glabrous; the radix paeoniae alba blooms from May to June and fruits in August; the fruit is composed of 3-5 small sub-fruits; the roots can be used as medicines; the radix paeoniae alba is harvested in summer and autumn; the radix paeoniae alba is cooked in a boiler till no hard core exists and then the peel is removed, or the radix paeoniae alba is peeled and then is cooked and aired for standing by; the radix paeoniae alba tastes bitter and sour and has tiny cold nature; the radix paeoniae alba has the effects of calming liver, relieving pain, nourishing blood for regulating menstruation, restraining yin and stopping sweat; the radix paeoniae alba with axillary bud stem is taken as an explant for establishing an in vitro rapid propagation system for radix paeoniae alba and establishing a basis for large-scale production of radix paeoniae alba. According to the invention, the radix paeoniae alba with axillary bud stem is taken as the explant, the tissue-culture rapid propagation for radix paeoniae alba is realized through the processes of axillary bud induction, enrichment culture, root seedling induction and test-tube plantlet domestication transplanting and a technical support is supplied for the industrial development of the radix paeoniae alba.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Radix Paeoniae Alba. Background technique [0002] Also known as Radix Paeoniae Alba: Radix Paeoniae Alba, Hang Shao, Chuan Shao, Milli Shao. It is a perennial herb with a height of 40-80 cm. Root hypertrophy, cylindrical, surface dark brown or brownish yellow. Stem erect, smooth and glabrous. The leaves are alternate, the lower cauline leaves, the leaflets are narrowly ovate, elliptic or lanceolate, the apex is pointed, the base is cuneate, and the leaf surface is glabrous. It blooms from May to June. The flowers are large and beautiful, white, sometimes with dark purple or red spots. Several flowers are born on the top or end of the branches, and the petals are obovate. Bearing fruit in August, the fruit is composed of 3-5 small fractes, glabrous, with hooked apex curved outward. The root...

AI Technical Summary

Problems solved by technology

The traditional method of obtaining Radix Paeoniae Alba is to dig wild plant resources in the field, which is easy to cause soil erosion and severe soil desertification

In addition, the seeds of Radix Paeoniae Alba mainly come from wild species, the varieties are mixed, the seeds are scarce, and the germination rate is extremely low. Wild Radix Paeoniae Alba resources can no longer meet the needs of large-scale and standardized production, which has become the biggest bottleneck in the development of Radix Paeoniae Alba.