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Tissue culture rapid propagation method of aristolochia debilis

A technology of tissue culture and A. chinensis, which is applied in the field of tissue culture and rapid propagation of A. chinensis, can solve the problems of soil desertification, low germination, scarcity of seeds, etc., and achieve the effects of fast growth and germination, convenient operation and pure variety.

Inactive Publication Date: 2018-01-19
罗荣棋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional way to obtain Aokimi is to dig wild plant resources, causing soil erosion and severe soil desertification
In addition, the seeds of Aokimba mainly come from the wild, the varieties are mixed, the seeds are scarce, and the germination is extremely low. The wild Aokimba resources can no longer meet the needs of large-scale and standardized production, which has become the biggest bottleneck hindering the development of Aokimba industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Treatment of explants: Select the stem segment with axillary buds from a robust plant of A. chinensis as explants, cut off the leaves, cut into 2.0 long segments with 3 axillary buds, and wash them with washing powder water for 4 hours , and then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 2 hours, then place it in an ultra-clean workbench, first disinfect it with 70% ethanol for 6 seconds, and then wash it with sterile water for 4 seconds Then, disinfect with 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water for 5 times, and then dry the water droplets on the surface with sterile filter paper before use.

[0017] (2) Clumping bud guide: cut the stems of the axillary buds of Aokita chinensis treated in step (1) into 1.25 cm pieces and inoculate them on the induction medium, culture them in the dark at 26°C for 5 days, and then place them in the I...

Embodiment 2

[0022] (1) Treatment of explants: Select the stem segment with axillary buds from a robust plant of A. chinensis as explants, cut off the leaves, cut into 6.0 long segments with 4 axillary buds, and wash them with washing powder water for 5 hours , and then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 4 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 12 seconds, and then wash it with sterile water for 6 hours Then, sterilize with 0.1% mercuric chloride solution for 8 minutes, rinse with sterile water for 6 times, and then dry the water droplets on the surface with sterile filter paper before use.

[0023] (2) Tufted bud guide: Cut the stems with axillary buds treated in step (1) into 2.8cm pieces and inoculate them on the induction medium, culture them in total darkness at 26°C for 5 days, and then place them under light for 11 hours a day...

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Abstract

The invention discloses a tissue culture rapid propagation method of aristolochia debilis. According to the tissue culture rapid propagation method, an aristolochia debilis in-vitro rapid propagationsystem is established by taking an aristolochia debilis stem with axillary buds as an explant and a foundation is laid for large-scale production of the aristolochia debilis. The tissue culture rapidpropagation method takes the aristolochia debilis stem with the axillary buds as the explant and tissue culture rapid propagation of the aristolochia debilis is realized through a process of inducingclustered shoots, carrying out propagation culture, inducing roots and seedlings, domesticating and transplanting test-tube seedlings and the like; technical supports are provided for industrialized development of the aristolochia debilis. The rapid propagation system of the aristolochia debilis is obtained by utilizing a simple technical means; germchits have a high seedling emergency rate, growrapidly and have luxuriant leaves and strong roots, and rich harvest and high yield.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Akira sinensis. Background technique [0002] Qingmuxiang is also known as Tianxian vine, Mizukixiang, Sha medicine. It is a perennial herbaceous vine. Root cylindrical, 0.5-1.5 cm in diameter, surface taupe or taupe. Stems erect when first born, later climbing. The leaves are alternate, the leaves are triangular and oblong, and the two sides of the base protrude like ears. Flowers solitary in leaf axils, obliquely funnel-shaped, purple-green, sickle-shaped and curved. Fruits from September to October, the fruit is nearly spherical, about 6 cm long, about 4 cm in diameter, with 6 ribs, and cracks when ripe. Seeds are flat, obtusely triangular, about 4 cm long and wide, with white membranous broad wings on the margin. The roots are excavated in spring and autumn, and dried in the sun for la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 罗荣棋
Owner 罗荣棋
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