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Fluorescent large-grain-size dendrimer and preparation method thereof

A dendritic and macromolecular technology, which is applied in the field of fluorescent large particle dendritic macromolecules and its preparation, can solve the problems of tedious and time-consuming purification process, harsh operating conditions, and toxicity in the production process, so as to achieve rich surface groups, The effect of simple preparation method and easy functionalization

Active Publication Date: 2017-12-15
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention lies in the problems of cumbersome and time-consuming preparation and purification process of the existing large-diameter dendrimers, harsh operating conditions, high toxicity, low efficiency and high cost in the production process, and further provides a A simple method, using low algebra and easy-to-prepare dendrimers and carbon quantum dots (Carbon dots, referred to as CDs) to assemble a combination of large-size dendrimers with fluorescent properties, the process flow is simple, The production process is environmentally friendly, high efficiency, low cost, and has a good fluorescent effect

Method used

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  • Fluorescent large-grain-size dendrimer and preparation method thereof
  • Fluorescent large-grain-size dendrimer and preparation method thereof
  • Fluorescent large-grain-size dendrimer and preparation method thereof

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Embodiment 1

[0045] The preparation method of the fluorescent large particle size dendrimers of the present embodiment comprises the following steps:

[0046] S1. Preparation of fluorescent carbon quantum dots

[0047] Dissolve 1.3g of citric acid in 25ml of formamide, add it into a 100ml Teflon-lined autoclave, and react at 200°C for 12h. After the reaction, the product was collected with 100ml of acetone sedimentation, dispersed in 50ml of methanol and filtered, evaporated to dryness and dispersed in 15ml of methanol to make a carbon quantum dot solution of 0.008mmol / ml;

[0048] Preparation of S2 and 3 generation PAMAM dendrimers

[0049] Using methyl acrylate and ethylenediamine as raw materials, the required third-generation PAMAM dendrimers were obtained through six Michael addition reactions and condensation reactions, and stored at low temperature. The specific preparation steps are as follows:

[0050] S2-1, PAMAM synthesis of 0.5G

[0051] Take 2.2ml of ethylenediamine, 24ml o...

Embodiment 2

[0073] The preparation method of the fluorescent large particle size dendrimers of the present embodiment comprises the following steps:

[0074] S1. Preparation of fluorescent carbon quantum dots

[0075] Dissolve 1.3g of citric acid in 25ml of formamide, add it into a 100ml Teflon-lined autoclave, and react at 200°C for 12h. After the reaction, the product was collected by sedimentation with 110ml of acetone, dispersed with 40ml of methanol and filtered, evaporated to dryness and dispersed in 30ml of methanol to make a carbon quantum dot solution of 0.004mmol / ml;

[0076] Assembly of S2 and 3 generation PAMAM dendrimers and carbon quantum dots

[0077] Get the carbon quantum dot solution prepared by 10ml step S1 and disperse it in 20ml ethanol, add the 3rd generation PAMAM dendrimer prepared by step S2 in 1.5g embodiment 1 after ultrasonic dispersion, add 0.8mmolEDC after stirring in an ice-water bath. HCl and 1.2mmol NHS, nitrogen gas for 15min, then react in an ice-water...

Embodiment 3

[0079] The preparation method of the fluorescent large particle size dendrimers of the present embodiment comprises the following steps:

[0080] S1. Preparation of fluorescent carbon quantum dots

[0081] Dissolve 1.3g of citric acid in 25ml of formamide, add it into a 100ml Teflon-lined autoclave, and react at 220°C for 16h. After the reaction, the product was collected by sedimentation with 100ml of acetone, dispersed in 45ml of methanol and filtered, evaporated to dryness and dispersed in 15ml of methanol to make a carbon quantum dot solution of 0.008mmol / ml;

[0082] Assembly of S2 and 3 generation PAMAM dendrimers and carbon quantum dots

[0083]Get the carbon quantum dot solution prepared by 10ml step S1 and disperse it in 20ml ethanol, add the 3rd generation PAMAM dendrimer prepared by step S2 in 2.0g embodiment 1 after ultrasonic dispersion, add 1.6mmolEDC after stirring in ice-water bath. HCl and 2.0mmol NHS, nitrogen gas for 15min, then react in an ice-water bath ...

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Abstract

The invention discloses a fluorescent large-grain-size dendrimer and a preparation method thereof. Specifically, a carbon quantum dot containing carboxyl is used as a fluorescent core and a third-generation PAMAM (Polyamidoamine) dendrimer with an end part containing amino is assembled on the surface of the carbon quantum dot through condensation reaction. The prepared fluorescent large-grain-diameter dendrimer has a macromolecular fluorescent effect and a relatively great grain diameter; various properties of the fluorescent large-grain-diameter dendrimer are better than that of the third-generation dendrimer. A preparation process of the method is relatively simple and easy to operate, low in cost, good in repeatability and safe and environmentally friendly; a product has a good fluorescent property and can be used for various fields including medicine carrying, fluorescent imaging, gene transfection and the like.

Description

technical field [0001] The invention relates to the technical field of biological carrier materials, in particular to a fluorescent large-diameter dendritic macromolecule and a preparation method thereof. Background technique [0002] In recent years, due to its single molecular weight distribution, high functional group density on the surface and easy functionalization and many other advantages, dendrimers have been increasingly used in many biomedical related fields such as drug delivery and gene transfection. . Compared with traditional linear macromolecules, dendritic macromolecules have definite molecular weight and molecular size. When the algebra is low, dendritic macromolecules are generally open molecular configurations. When the algebra reaches more than four generations, the molecules will change. It is a spherical three-dimensional structure that is tight on the outside and loose on the inside. However, with the development of this technology, there are more an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/02C09K11/65C09K11/02
CPCC08G73/028C09K11/025C09K11/65
Inventor 于冰丛海林杨振单玉领
Owner QINGDAO UNIV
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