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A new natural product polysaccharide lsm-x from Ceratus cockscomata and its application

A technology of LSM-X and natural products, which is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, can solve the problems such as no reports on the polysaccharide LSM-X of Cockscomb sp. The effect of promoting B cell proliferation

Active Publication Date: 2019-09-06
CHINA WEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there has not been any report on the fine structure and immunomodulatory activity of polysaccharide LSM-X from C. cockscomata LSM-X and its application

Method used

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  • A new natural product polysaccharide lsm-x from Ceratus cockscomata and its application
  • A new natural product polysaccharide lsm-x from Ceratus cockscomata and its application
  • A new natural product polysaccharide lsm-x from Ceratus cockscomata and its application

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Embodiment 1

[0025] The separation and extraction of embodiment 1 cockscomb polysaccharide LSM-X

[0026] 1.1 Separation and extraction of polysaccharide LSM-X from C. cockscomata

[0027] 1.1.1 Extraction of Crude Polysaccharides from Cockroach Cerules by Hot Water Extraction

[0028] The pulverized Cockscomb sporocarp powder and distilled water were boiled in a water bath at 100°C for 3 hours at a volume ratio of 1:3, boiled 3 times, and the supernatant was collected and concentrated to 200ml. Three times the volume of absolute ethanol was added to form a flocculent precipitate, which was collected and dried. This process is to use the Sevage method to remove the protein in the extract to obtain the crude polysaccharide of C. crestella.

[0029] 1.1.2 DEAE-cellulose column chromatography separation and purification of crude polysaccharides

[0030] Weigh 50.00 g of DEAE-cellulose, stir evenly with distilled water, and soak for 24 hours to remove suspended particles. Then soak in 0.5m...

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Abstract

The invention discloses a novel natural product-lactikporus supharells (Fr.)Murr. polysaccharide LSM-X and an application thereof. The lactikporus supharells (Fr.)Murr. polysaccharide LSM-X is heteropolysaccharide containing three monosaccharides, namely, beta-D-glucose, alpha-D-galactose and D-lyxose in a ratio of 4: 2: 1, the average molecular weight is about 14,695 Da, the main chain is 1,6-connected beta-D-glucose, 4-O has a side chain, and the polysaccharide has two 1,6-connected alpha-D-galactose residues and one 1-connected d-lyxose. A certain dose of the lactikporus supharells (Fr.)Murr. polysaccharide LSM-X has significant immunoregulatory activity, and when the concentration of LSM-X is 10 mu g / mL, the B and T cellular proliferation inhibition rate is the highest; proliferation of the cell B can be promoted by affecting three stages of the cell cycle. When the concentration of LSM-X is 5 mu g / mL, the percentage of the cell B in the phase G0 / G1 is the lowest.

Description

technical field [0001] The invention belongs to the technical field of fungal polysaccharide application, and in particular relates to a new natural product polysaccharide LSM-X from cockscomb bacterium and its application. Background technique [0002] Polysaccharides are a class of biomacromolecules ubiquitous in organisms. Modern biochemical studies have found that polysaccharides can participate in the cytoskeleton and some bioactive molecules; such as cellulose and chitin that maintain cell structure; starch and glycogen that provide energy sources. The development of polysaccharides is late, and the structure is complex and diverse, so it is difficult to study its structure and activity. [0003] In the past 20 years, research reports on the biological activities of polysaccharides have mainly focused on immune regulation, anti-tumor, anti-virus, anti-oxidation, and hypoglycemic effects, and their effects are multi-path, multi-link, and multi-target. [0004] Cocksco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/02
Inventor 侯怡铃丁祥侯万儒刘露朱洪庆赵大群
Owner CHINA WEST NORMAL UNIVERSITY
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