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A kind of immunomodulatory active peptide and its preparation method and application

A technology of immunomodulatory activity and active peptides, which is applied in the field of immunomodulatory active peptides and their preparation, can solve the problem of fewer active peptide products, achieve the effects of increasing product added value, good immune regulatory activity, and promoting sustainable development

Active Publication Date: 2022-03-04
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many reports about soybean active peptides, there are still few active peptide products with high activity.

Method used

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  • A kind of immunomodulatory active peptide and its preparation method and application
  • A kind of immunomodulatory active peptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation, separation and structural identification of immunomodulatory active peptides

[0022] 1. Preparation and separation of immunomodulatory active peptides

[0023] 1) Material selection: choose soybean protein isolate.

[0024] 2) Preparation: Add 10 times the mass of soybean protein isolate in water, then stir to disperse.

[0025] 3) Enzymatic hydrolysis: add 0.5% alkaline protease according to the quality of soybean protein isolate, pH 8.5, temperature 50°C, enzymolysis for 3 hours, then add 0.5% protease SmPA, pH 7.0, temperature 50°C, enzymolysis 3 hours. Raise the temperature to 90°C and keep for 20 minutes to inactivate the enzyme, centrifuge to obtain the supernatant of the protein hydrolyzate, filter it with an ultrafiltration membrane with a molecular weight cut-off of 5000 Daltons, collect the permeate and spray dry to obtain the soybean protein hydrolyzate.

[0026] 4) Column purification: the soybean protein hydrolyzate is purified b...

Embodiment 2

[0037] 1. Preparation and separation of immunomodulatory active peptides

[0038] 1) Material selection: choose soybean protein isolate.

[0039] 2) Preparation: Add water with 5 times the mass of soybean protein isolate, then stir to disperse.

[0040] 3) Enzymatic hydrolysis: Add 0.1% alkaline protease according to the quality of soybean protein isolate, pH 7.5, temperature 30°C, enzymolysis for 1 hour, then add 0.1% protease SmPA, pH 6.0, temperature 40°C, enzymolysis 1 hour. Raise the temperature to 90°C for 20 minutes to inactivate the enzyme, centrifuge to obtain the supernatant of the protein hydrolyzate, filter it with an ultrafiltration membrane with a molecular weight cut-off of 5000 Daltons, and collect the permeate and spray dry it.

[0041] 4) Column purification: the soybean protein hydrolyzate is purified by a C18 reverse-phase chromatographic column (15×460 mm), and gradient eluted with water and ethanol aqueous solution, specifically, the volume fraction of ...

Embodiment 3

[0044] 1. Preparation and separation of immunomodulatory active peptides

[0045] 1) Material selection: choose soybean protein isolate;

[0046] 2) Preparation: Add water with 20 times the mass of soybean protein isolate, then stir to disperse.

[0047] 3) Enzymatic hydrolysis: add 1.0% alkaline protease according to the quality of soybean protein isolate, pH 8.5, temperature 50°C, enzymolysis for 5 hours, then add 1.0% self-made protease SmPA, pH 7.0, temperature 50°C, enzyme solution for 5 hours. Raise the temperature to 90°C for 20 minutes to inactivate the enzyme, centrifuge to obtain the supernatant of the protein hydrolyzate, filter it with an ultrafiltration membrane with a molecular weight cut-off of 5000 Daltons, collect the permeate and spray dry to obtain the soybean protein hydrolyzate.

[0048] 4) Column purification: the soybean protein hydrolyzate is purified by a C18 reverse-phase chromatographic column (15×460 mm), and gradient eluted with water and ethanol...

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Abstract

The invention discloses an immunoregulatory active peptide, its preparation method and application. Disperse soybean protein with water, add protease to hydrolyze, inactivate the enzyme at high temperature, centrifuge to obtain the supernatant of protein hydrolyzate, dry it after membrane separation, use reversed-phase chromatographic column to purify active peptide, gradient elution with water and ethanol solution, collect The fractions eluted with ethanol solution of specific concentration are concentrated and then purified by Sephadex LH-20 chromatographic column, the fractions eluted with ethanol solution of specific concentration are collected, concentrated and dried to obtain the target immunomodulatory active peptide. The invention utilizes the soybean protein to prepare the immunoregulatory active peptide, and the production rate is 30-92g / kg (the purity is 80-95%). The invention provides a new method for the preparation of immunomodulatory active peptides, which is of great significance for promoting the deep processing and utilization of soybean protein, increasing the added value of products, and promoting the sustainable development of this industry.

Description

Technical field: [0001] The invention belongs to the field of agricultural product processing, and in particular relates to an immunoregulatory active peptide and its preparation method and application. Background technique: [0002] Active peptides are important food functional factors, which have biological activities such as regulating immunity, anti-oxidation, and preventing osteoporosis. They are widely used in the production of health food and other products, and are very popular among consumers. Soybean protein is a bulk protein raw material, which is cheap and easy to obtain, and is very suitable for processing high-value products such as active peptides. The biological activity of an active peptide is highly related to its chemical structure, especially its amino acid sequence. Although there are many reports about soybean active peptides, there are still few active peptide products with higher activity. Therefore, it is necessary to carry out research on the prep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/20A61K38/08A61P37/02A23L33/18
CPCC07K7/06C12P21/06A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324
Inventor 杨宝蒋跃明
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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