Sulfated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof
A technology of polysaccharide sulfuric acid and hollyhock, which is applied to organic active ingredients, medical preparations containing active ingredients, food ingredients containing polysaccharides/gum, etc., can solve the problems of less active research and achieve the goal of improving immune activity and good ecological environment Protective significance, effect of significant immunomodulatory activity
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Embodiment 1
[0030] The preparation method of the polysaccharide sulfated modification product of the stem and leaf of hollyhock comprises the following steps:
[0031] (1) Preparation of crude polysaccharides from stems and leaves of hollyhock
[0032] Take 100 g of dry dregs of hollyhock stems and leaves extracted by ethanol reflux, add water 30 times the weight of the dregs, and extract by reflux in a water bath at 100°C for 3 times, each time for 1 h. Filter, combine the filtrates, and concentrate under reduced pressure. Remove protein by Sevag method, centrifuge (4000r / min, 10min), take the supernatant, add four times the volume of absolute ethanol, alcohol precipitation overnight, filter with suction, wash the precipitate three times with absolute ethanol, acetone, ether, 50°C drying to obtain the crude polysaccharide from the stems and leaves of the hollyhock.
[0033] (2) Fractionation of crude polysaccharides from stems and leaves of hollyhock
[0034] Weigh 3 g of the crude po...
Embodiment 2
[0037] Example 2 Determination of total sugar content and degree of substitution of hollyhock stem and leaf polysaccharide sulfated modification product
[0038] 1, with D-glucose anhydrous as standard substance, adopt the phenol-sulfuric acid method to measure the total sugar content of the crude polysaccharide of hollyhock stem and leaf before and after sulfation modification; 2 SO 4As a standard product, the barium chloride-gelatin turbidimetric method was used to measure the sulfate content, and the degree of substitution (DS) was calculated according to the following formula.
[0039]
[0040] In the formula: S% is the content of sulfur element
[0041] 2. Monosaccharide composition analysis
[0042] Using trifluoroacetic acid hydrolysis, PMP derivatized polysaccharide samples, using mixed monosaccharides as standard products, using high performance liquid chromatography to determine the composition of monosaccharides according to the peak time, and drawing the stand...
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