A kind of stropharia polysaccharide and its preparation method and application
The technology of the polysaccharide and the polysaccharide is applied in the field of the polysaccharide and the preparation of the polysaccharide. The effect of enhancing phagocytic activity
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[0054] Example 1 Separation and extraction of the polysaccharide SR-1 of A. maxima
[0055] 1.1 Separation and extraction of polysaccharide SR-1
[0056] 1.1.1 Extraction of crude polysaccharide from Mushroom globulus by water extraction and alcohol precipitation
[0057] The pulverized M. globosa fruit body powder and distilled water were boiled in a water bath at 100° C. for 3 hours at a material-to-liquid ratio (W / V) of 1:3, boiled 3 times, and the supernatant was collected and concentrated to 200 mL. Three volumes of absolute ethanol were added to form a flocculent precipitate, which was collected and dried. The Sevage method was used to remove the protein in the extract to obtain the crude polysaccharide of Mushroom.
[0058] 1.1.2 Separation and Purification of Crude Polysaccharide of P. globosa
[0059]Weigh 50.00 g of DEAE-cellulose, stir evenly with distilled water, and soak for 24 hours to remove suspended particles. Then soaked in 0.5mol / L NaOH solution for 4 ho...
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