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A kind of milk mushroom polysaccharide and its preparation method and application

A technology of velvety milk mushroom and polysaccharide, applied in the application field of fungal polysaccharide, can solve problems such as no reports, achieve the best application prospect and commercial value, promote the secretion of immunoglobulin by B cells, and improve the effect of utilization value.

Active Publication Date: 2021-11-26
CHINA WEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the milk mushroom is mainly focused on the analysis of the bioactive components of the milk mushroom extract, and there is no report on the fine structure and immune activity of milk mushroom polysaccharides and its application.

Method used

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  • A kind of milk mushroom polysaccharide and its preparation method and application
  • A kind of milk mushroom polysaccharide and its preparation method and application
  • A kind of milk mushroom polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Separation and extraction of polysaccharide LV-1 of lactobacillus versicolor

[0059] 1. Separation and extraction of polysaccharide LV-1 from Lactobacillus versicolor

[0060] 1.1. Water extraction and alcohol precipitation method to extract crude polysaccharides from Lactaria versicolor

[0061] Weigh 200g of dried milk mushroom fruiting bodies and crush them, add the crushed milk mushroom fruiting bodies and distilled water in a ratio of 1:3 to the beaker, and put them in a water bath at 90°C for 6 hours, cook three times, and let them stand Leave overnight, centrifuge to collect the supernatant, use a rotary evaporator to concentrate the supernatant to a viscous state, add 4 times the volume of absolute ethanol to make it precipitate, collect the precipitate and dry it. At the same time, the protein in the extract was removed by the Sevage method to obtain the crude polysaccharide of Lactaria versicolor.

[0062] 1.2. DEAE-cellulose column chromatogr...

Embodiment 2

[0097] Example 2: Study on the Immunomodulatory Activity of Lactobacillus versicolor LV-1

[0098] The immunomodulatory activity of polysaccharide LV-1 from L. versicolor velvet was determined by CCK-8 method in vitro.

[0099] 1. Reagents

[0100] CCK-8 kit, RPIM1640, FBS, DMSO, double antibodies, etc. are all commercially available products.

[0101] 2. Instrument

[0102] microplate reader; flow cytometer.

[0103] 3. Method

[0104] 3.1. The effect of LV-1 on the proliferation of immune cells (B cells, T cells and RAW264.7 cells)

[0105] The effect of LV-1 proliferation on B, T and RAW264.7 cells was determined by CCK-8 method. The experiment was set as drug group (LV-1), lipopolysaccharide (LPS) positive control group (LPS), blank control group (Control), and pure blank group (only cell culture medium was added), with 6 biological repetitions for each group . Firstly, B and T lymphocytes and RAW264.7 cells were prepared into cell suspension, and in order to elimin...

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Abstract

The invention discloses a milk mushroom polysaccharide and its preparation method and application. The polysaccharide is extracted from the fruit body of Lactaria versicolor, which is a heteropolysaccharide composed of α-D-galactose and α-D-glucose, wherein the molar ratio of the residues of α-D-galactose and glucose is 2: 1. The polysaccharide has significant immunoregulatory activity, can be used to prepare medicines, and can also be used to prepare immunity-enhancing health products and foods, has good application prospects and commercial value, and can also improve the utilization value of the milk mushroom.

Description

technical field [0001] The invention belongs to the technical field of fungal polysaccharide applications, and in particular relates to a polysaccharide from Lactaria versicolor and its preparation method and application. Background technique [0002] Due to the inherent potential and efficacy contained in Chinese herbal medicine, in recent years, the country has paid more and more attention to the research of traditional Chinese medicine. As an important part of traditional Chinese medicine, large edible and medicinal fungi have been studied most extensively. my country has a vast territory and has bred a wealth of edible and medicinal fungi resources. A large number of edible and medicinal fungi have been included in "Compendium of Materia Medica" and other works, such as Ganoderma lucidum, Morchella, and Poria cocos. Medicinal fungi not only have the effects of nourishing qi and strengthening the body, dispelling diseases and stimulating the menstrual flow, but also have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04A61P35/00A23L31/00A23L33/125
CPCA23V2002/00A23L31/00A23L33/125A61P35/00A61P37/04C08B37/0003C08B37/006A23V2200/30A23V2250/51
Inventor 侯怡铃丁祥宋志强唐贤朱淼杨彤陈茜朱洪庆
Owner CHINA WEST NORMAL UNIVERSITY
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