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Preparation and application of dura mater repairing material

A technology for repairing materials and dura mater, which is applied in the field of biomaterials and medical and health care. It can solve the problems of local fluid accumulation or aseptic inflammation, wrapping reaction, poor biocompatibility, etc., achieve low immunogenicity and purity, and promote tissue The effect of repairing and super absorbing ability

Inactive Publication Date: 2017-12-15
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although autologous tissue repair materials have good biocompatibility and low immune response, their sources are limited and expensive
Allogeneic and xenogeneic biological materials are readily available, but there is a risk of immune rejection
Artificial synthetic materials, the hydrophilicity is not ideal, the degradation products are mostly acidic, and the biocompatibility is relatively poor. After a long storage time, it is easy to form an encapsulation reaction, which can cause local fluid accumulation or aseptic inflammation and other complications.

Method used

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  • Preparation and application of dura mater repairing material
  • Preparation and application of dura mater repairing material
  • Preparation and application of dura mater repairing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Select bovine Achilles tendon rich in collagen, remove the fascia, and freeze at -20°C for 6 hours. The frozen tissue is then processed for sectioning.

[0028] 2. Weigh 100g of beef Achilles tendon slices, soak and rinse in water for 10min, and drain the excess water; prepare 2L of 0.05mol / L phosphate buffer solution, adjust the pH to 7, and weigh 5g of pepsin enzyme into it after completion , mix well.

[0029] 3. Add the rinsed tissue to the above enzyme solution, put it in a shaker, and keep it at 20°C for 8 hours; the ratio of bovine Achilles tendon slices to enzyme solution is 5:100.

[0030] 4. Enzyme inactivation: Take out the reactant from the shaker, rinse it with water several times, drain off the excess water, add it to 2% ammonium nitrate and 0.5% sodium hydroxide solution, the solution volume is 5L, turn on the shaker, at 30 Keep at ℃ for 1h.

[0031] 5. Virus inactivation: Take out the reactant from the shaker, rinse it with water several times, dra...

Embodiment 2

[0039] 1. Select pigskin rich in collagen, remove impurities, and freeze at -20°C for 24 hours. The frozen tissue is then processed for sectioning.

[0040] 2. Weigh 500g of pigskin slices, soak and rinse in water for 20min, and drain excess water; prepare 20L of 0.05mol / L phosphate buffer solution, adjust the pH to 6, weigh 1g of ficin and add it to fully Mix well.

[0041] 3. Add the rinsed tissue to the above enzyme solution, put it in a shaker, and keep it at 30°C for 20 hours; the ratio of bovine Achilles tendon slices to enzyme solution is 1:40.

[0042] 4. Enzyme inactivation: Take out the reactant from the shaker, rinse it with water several times, drain off the excess water, add it to 5% ammonium nitrate solution, the solution volume is 10L, turn on the shaker, and keep it at 20°C for 2h.

[0043] 5. Virus inactivation: Take out the reactant from the shaker, rinse it with water several times, drain the excess water, add it to 6% sodium sulfate and 0.5% sodium hydrox...

Embodiment 3

[0049] 1. Select cowhide rich in collagen, remove impurities, and freeze at -20°C for 16 hours. The frozen tissue is then processed for sectioning.

[0050] 2. Weigh 1000g of cowhide slices, soak and rinse in water for 30min, drain excess water; prepare 50L of 0.05mol / L phosphate buffer, adjust the pH to 7, weigh 35g of cysteine ​​protease and add it , mix well.

[0051] 3. Add the rinsed tissue to the above enzyme solution, put it in a shaker, and keep it at 40°C for 8 hours; the ratio of bovine Achilles tendon slices to enzyme solution is 1:50.

[0052] 4. Enzyme inactivation: Take out the reactant from the shaker, rinse it with water several times, drain off the excess water, add it to 3% ammonium nitrate and 0.2% sodium hypochlorite solution, the solution volume is 20L, turn on the shaker, at 30°C Keep for 5h.

[0053] 5. Virus inactivation: Take out the reactant from the shaker, rinse it with water several times, drain off the excess water, add it to 3% sodium sulfate ...

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Abstract

The invention relates to preparation and application of a dura mater repairing material, belonging to the fields of biological materials, medical treatment and public health. The preparation of the dura mater repairing material specifically comprises the steps of extracting collagen, preparing slurry, carrying out freeze-drying, crosslinking and sterilization on a dura mater, and the like. The obtained dura mater repairing material has a complete triplex structure and the advantages of high mechanical property, good biocompatibility, biodegradability, controllability and the like and can be taken as a substitute of a dura mater and a spinal film.

Description

technical field [0001] The invention belongs to the field of biological materials and medical care, and in particular relates to the preparation and application of a dura mater repair material. Background technique [0002] The dura mater is a relatively strong connective tissue film. The natural dura mater structure is mainly composed of fibroblasts and collagen fibers. It is located in the outermost layer of the brain and spinal cord membrane structure. It is an important barrier to protect brain tissue. The structure and functional activities of the system are of great significance. The dura mater is composed of dense connective tissue, thick and tough, forming a long cylindrical dural sac. Attached to the edge of the foramen magnum above, continuous with the dura mater, downward to form a blind end at the level of the second sacral vertebra, and attached to the coccyx by the filum terminale. There are cerebrospinal fluid, spinal cord and 31 pairs of spinal nerve roots ...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/58A61L27/56A61L27/50
CPCA61L27/24A61L27/50A61L27/56A61L27/58A61L2430/32A61L2430/40
Inventor 闫瑞国富勇
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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