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Preparation and application of stitchable dura mater repairing material

A technology for repairing materials and dura mater, which is applied in the fields of biomaterials and medical and health care, and can solve problems such as increased burden on families, insufficient mechanical strength, and material tearing, and achieves the effects of low price, simple preparation process, and reduced complications

Active Publication Date: 2018-01-09
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to prevent cerebrospinal fluid leakage, in clinical application, the repair materials are selected to be sutured to reduce the risk of leakage. However, most of the above repair materials are not suitable for suturing, mainly because their mechanical strength is not enough, and the material is easy to tear during the suturing process. Invisibly causing pain to patients and increasing the burden on the family, so it is urgent to develop a suturable dura mater repair material with good biocompatibility, wide sources and low cost

Method used

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  • Preparation and application of stitchable dura mater repairing material
  • Preparation and application of stitchable dura mater repairing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1 Select bovine Achilles tendon rich in collagen, remove the fascia, freeze at -20°C for 6 hours, and then slice the frozen tissue;

[0028] Weigh 200g of beef Achilles tendon slices, soak and rinse in water for 30min, and drain excess water; at the same time, prepare 5L of 0.05mol / L phosphate buffer solution, adjust the pH to 7, weigh 5g of pepsin and add it to fully mix;

[0029] 2 Add the rinsed bovine Achilles tendon slices to the above enzyme solution, put them in a shaker, and keep at 30°C for 10 hours; the ratio of bovine Achilles tendon slices to enzyme solution is 4:100;

[0030] 3. Enzyme inactivation: Take out the reactants from the shaker, rinse with water several times, drain excess water, add to 1% ammonium nitrate and 0.4% sodium hydroxide solution, the solution volume is 10L, turn on the shaker, Keep it down for 0.5h;

[0031] 4. Virus inactivation: Take out the reactant from the shaker, rinse it with water several times, drain the excess water, add it t...

Embodiment 2

[0041] 1. Select collagen-rich trotters, remove impurities, freeze at -20°C for 12 hours, and then slice the frozen tissue;

[0042] Weigh 300g of tissue slices, soak and rinse in water for 20min, and drain excess water; at the same time, prepare 10L of 0.03mol / L phosphate buffer solution, adjust the pH to 6, weigh 1g of ficin and add it to it, and mix well ;

[0043] 2 Add the washed tissue slices to the above enzyme solution, put them in a shaker, and keep at 20°C for 5 hours; the ratio of tissue slices to enzyme solution is 3:100;

[0044] 3 Enzyme inactivation: Take out the reactant from the shaker, rinse it with water several times, drain off excess water, add it to 1% ammonium nitrate and 0.4% sodium hypochlorite solution, the solution volume is 50L, turn on the shaker, and keep it at 20°C 5h;

[0045] 4. Virus inactivation: Take out the reactant from the shaker, rinse it with water several times, drain off excess water, add it to 3% sodium thiosulfate solution, the vo...

Embodiment 3

[0053] 1 Select collagen-rich fish skin, remove impurities, freeze at -20°C for 40 hours, and then slice the frozen tissue;

[0054] Weigh 1000g of tissue slices, soak and rinse in water for 20min, drain excess water; prepare 50L of 0.05mol / L phosphate buffer at the same time, adjust the pH to 6, weigh 25g of cysteine ​​protease and add it after completion, Mix well;

[0055] 2 Add the washed tissue slices to the above enzyme solution, put them in a shaker, and keep at 35°C for 8 hours; the ratio of tissue slices to enzyme solution is 2:100;

[0056] 3 Enzyme inactivation: Take out the reactant from the shaker, rinse it with water several times, drain off excess water, add it to 0.5% sodium nitrate and 1% potassium hydroxide solution, the solution volume is 50L, turn on the shaker, Keep it down for 1h;

[0057] 4 Virus inactivation: Take out the reactant from the shaker, rinse it with water several times, drain the excess water, add it to 1.0% sodium sulfate solution and 0.5...

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Abstract

The invention relates to a preparation and application of a stitchable dura mater repairing material, and belongs to the field of a biomaterial and medical treatment and public health. The invention specifically discloses a preparation and application of a stitchable dura mater repairing material. The preparation comprises: extraction of target collagen, preparation of a compact layer, preparationof a porous layer, freeze-drying of dura mater, crosslinking and sterilization. The dura mater repairing material has a double-layer composite structure, and is good in mechanical property, is stitchable, and can effectively prevent bleeding of a cerebrospinal fluid. The material is high in purity and has a triple helical structure integratedly maintained in the compact layer. The material is good in biological activity and biological compatibility and controllable in degradation, and is a good substitute for cerebral dura mater and spinal dura mater.

Description

technical field [0001] The invention belongs to the field of biological materials and medical care, and in particular relates to the preparation and application of a suturable dura mater repair material. Background technique [0002] As we all know, both dura mater and dura mater are indispensable parts of the human body and are the basic components of maintaining life. The dura mater is a relatively strong connective tissue film, mainly composed of fibroblasts and collagen fibers, located in the brain and The outermost layer of the spinal cord membrane is an important barrier to protect the brain tissue, and is of great significance for maintaining the structure and functional activities of the nervous system. The dura mater is composed of dense connective tissue, thick and tough, forming a long cylindrical dural sac. Attached above is to the edge of the foramen magnum, continuous with the dura mater, and attached to the coccyx by the filum terminale. There are cerebrospi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/24A61L27/20A61L27/50A61L27/56A61L27/58
Inventor 闫瑞国富勇
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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