Breeding method for cold-resistant okra
A cold resistance and seed technology, applied in botany equipment and methods, seed immunization, seed and rhizome treatment, etc., can solve the problems of low planting mutation rate, easy loss of genetic traits, uncertain variation direction, etc., to improve immunity Strength and growth rate, improve survival rate, improve the effect of absorption
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Embodiment 1
[0019] A breeding method for cold-resistant okra, comprising the following steps:
[0020] (1) Seed storage: detoxify the okra seeds and immerse them in the gibberellin solution for 5.5 minutes to increase the cell viability of the okra seeds, then mix the okra seeds with the culture medium, transfer them to a sealed tank and store them at 9 Store at a constant temperature of ℃ for 9 days. The seeds absorb nutrients, phytohormones and other components in the culture medium, which can promote growth and improve seed immunity. Add colchicine solution to the culture medium, and then store at a constant temperature of 2.5°C for 15 days. Colchicine can induce cold resistance variation of seeds under low temperature conditions to produce stored seeds;
[0021] (2) Mutation breeding: first activate the stored seeds, put them into the atomization chamber and use the active selenium solution for atomization treatment. After the seeds absorb active selenium, the resistance to radiation ...
Embodiment 2
[0032] Present embodiment 2 is compared with embodiment 1, and step changes in the following aspects:
[0033] In the detoxification treatment described in step (1), the detoxification agent is sodium hypochlorite solution with a mass concentration of 60ppm, and the detoxification time is 18min; the described gibberellin solution has a mass concentration of 0.68%; the colchicum solution, its addition amount is 8.4% of the mass of the culture substrate, and its mass concentration is 2.7%.
[0034] Step (1) Seed storage, the method is:
[0035] The okra seeds were mixed with the culture medium, transferred to a sealed jar and stored at a constant temperature of 11°C for 9.5 days, and then stored at a constant temperature of 3.5°C for 16 days.
[0036] The culture substrate described in step (1), its preparation method is:
[0037] Weigh 28 parts of sawdust, 16 parts of lichen, 12 parts of peat, 9.6 parts of kudzu root powder, 6.7 parts of papaya skin, 5.8 parts of nandina, 7.2...
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