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Method for measuring effective ingredients in Huoxiang Zhengqi oral liquid

A technology for Zhengqi oral liquid and active ingredients, which is applied in the field of component content determination in Huoxiang Zhengqi oral liquid, can solve the problems of poor versatility and reproducibility, unsafe toxic reagents, different mobile phases, etc., and achieves good separation, Avoid the harm of human body and the environment, and the effect of short detection time

Inactive Publication Date: 2017-11-24
YANGTZE NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for measuring the active ingredients in Huoxiangzhengqi Oral Liquid, to solve the problems that the preparation of the test product is complicated and the existence of toxic reagents is unsafe, and the existing detection method There are problems such as long detection time, different mobile phases, complex operation, low efficiency, poor versatility and reproducibility

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  • Method for measuring effective ingredients in Huoxiang Zhengqi oral liquid
  • Method for measuring effective ingredients in Huoxiang Zhengqi oral liquid
  • Method for measuring effective ingredients in Huoxiang Zhengqi oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for determining active ingredients in Huoxiangzhengqi Oral Liquid (method for simultaneous determination of hesperidin / magnokiol / honokiol content)

[0044] Preparation of standard reference substance solution: Accurately weigh hesperidin, magnolol and honokiol standard reference substance, prepare the mixed reference substance solution shown in Table 1, filter through a 0.45 μm filter membrane, and set aside.

[0045] Table 1 Reference substance solution

[0046]

[0047] Preparation of the test solution: Accurately measure 5.0 mL of Huoxiang Zhengqi Oral Liquid stock solution and put it into a 10 mL volumetric flask, dilute to the mark with methanol, mix and filter with a 0.45 μm membrane filter, and set aside.

[0048] (1) System adaptability test

[0049] Take the reference substance solution and the test solution, and the chromatographic conditions are: (1) Chromatographic column: Waters Xbridge C18, 3.5 μm, 4.6×100mm; (2) Column temperature: 35°C; (3) ...

Embodiment 2

[0076] Parameter optimization

[0077] (1) Preparation method of the test article

[0078] The stock solution of the test sample is diluted 1 time, 2 times, 2.5 times, 5 times and no dilution with methanol or methanol to obtain 5 kinds of test products, which are analyzed according to the chromatographic conditions under the system suitability test. The results show that hesperidin, There was no significant difference in the contents of honokiol and magnolol, indicating that the solvent and dilution factor would not affect the test results. Water, 20%, 40%, 60%, 80% and 100% methanol and ethanol are respectively diluted twice for the test product, and analyzed according to the chromatographic conditions under the system suitability test, the results show that when the concentration of methanol and ethanol is low At 60%, compared with the test product stock solution and 100% methanol or ethanol dilution measurement results, the contents of hesperidin, honokiol and magnolol wer...

Embodiment 3

[0126] For 21 different batches of samples (provided by Taiji Group Fuling Pharmaceutical Factory Co., Ltd., batch numbers are shown in Table 21), the method of the present invention and the method of "Chinese Pharmacopoeia (2015)" were used to measure orange peel in Huoxiangzhengqi Oral Liquid respectively. The contents of glycosides, honokiol and magnolol were determined respectively. According to the data obtained from the analysis, it is judged whether there is a significant difference between the two methods. Follow the steps below for analysis.

[0127] Preparation of standard solution: Accurately weigh hesperidin, honokiol and magnolol standard reference substance, after diluting with methanol, prepare 2 parts of mixed reference substance solutions with different concentrations, as shown in Table 19:

[0128] Table 19 Reference substance solution

[0129]

[0130] 1, adopt the method analysis of the present invention (marked as method A)

[0131] 1) Preparation of...

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Abstract

The invention provides a method for simultaneously determining the contents of three components of hesperidin, magnolol and honokiol in Huoxiangzhengqi oral liquid by adopting a two-stage gradient elution method. The method of the present invention is suitable for high-performance liquid chromatography (HPLC), ultra-high performance liquid chromatography (UPLC) and other color chromatography instruments, and is suitable for octadecylsilane bonded silica gel (C18) of various types and specifications. Packed columns. The method of the present invention is also applicable to the simultaneous determination of the contents of the three components of hesperidin, magnolol and honokiol in Huoxiangzhengqi water, that is, the method of the present invention is suitable for the quality control of Huoxiangzhengqi oral liquid and Huoxiangzhengqi water. The method of the invention has good versatility on various liquid chromatographs and chromatographic columns with C18 as filler, and the sample preparation method is simple, high in stability, easy to operate, safe, fast, accurate and reliable, and high in efficiency.

Description

technical field [0001] The invention relates to a method for determining the content of components in Huoxiang Zhengqi Oral Liquid, in particular to a method for simultaneously measuring active ingredients in Huoxiang Zhengqi Oral Liquid, and belongs to the field of detection methods for components of Chinese patent medicines. Background technique [0002] The original prescription of Huoxiang Zhengqi Oral Liquid comes from the "Taiping Huimin Heji Bureau Prescription" in the Song Dynasty, and its active ingredients are magnolol, honokiol and hesperidin. After thousands of years of addition and subtraction evolution, it has been transformed from modern technology into a modern preparation that is easy to carry and has good curative effect. Patchouli is used as the king in this prescription, which aromatizes dampness and relieves heat, relieves exterior and cold, avoids filth and relieves vomiting, delights the spleen and harmonizes the middle, and treats both the exterior an...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 曹团武罗浩赵理
Owner YANGTZE NORMAL UNIVERSITY
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