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Preparation method of degradable silicon dioxide particle internally doped with polyphenol-metal mesh

A silica and mesh technology, applied in the field of preparation of silica nanoparticles, can solve the problems of DNA damage, protein denaturation, dose accumulation, etc., and achieve the effects of uniform size, increased porosity, and simple preparation process

Active Publication Date: 2017-11-24
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the compact structure of silica, silica particles have quite good thermal stability. It takes a long time to decompose in cells and living bodies, and it is easy to cause dose accumulation in tissues and organs, thereby causing DNA damage, Protein denaturation, etc. and lead to a series of adverse results

Method used

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  • Preparation method of degradable silicon dioxide particle internally doped with polyphenol-metal mesh
  • Preparation method of degradable silicon dioxide particle internally doped with polyphenol-metal mesh
  • Preparation method of degradable silicon dioxide particle internally doped with polyphenol-metal mesh

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Effect test

Embodiment 1

[0037] A method for preparing degradable silica nanoparticles internally doped with a polyphenol-metal complex network structure, comprising the steps of:

[0038] SiO 2 -EGCG-Fe

[0039] a) Add 430 μL TEOS and 8.66 mg EGCG (8.66% doped mass ratio) into ethanol-water solution with a volume ratio of 60:1, stir for 10 min to mix well; add 0.5 mL ammonia water dropwise, Reacted at 25°C for 0.5 h to obtain primary SiO containing polyphenol oligomers 2 .

[0040] b) Then add 1.01mg FeCl 3· 6H 2 O (the molar ratio is EGCG:Fe=5:1), continue to react in a water bath at 25°C for 5.5 h, after the reaction is complete. The reaction solution was taken out and placed in a centrifuge tube, centrifuged at 10,000 rpm for 10 min, the supernatant was taken out, the precipitate was washed twice with absolute ethanol, and then washed twice with ultrapure water, and the precipitate was dispersed in 10 mL twice distilled water to obtain the mixed Degradable silica nanoparticles mixed with EGC...

Embodiment 2

[0043] With TA as doped polyphenol molecules, Fe 3+ As metal ions, the effect of polyphenol doping ratio on the morphology and monodispersity of composite particles was investigated.

[0044] More specifically: SiO 2 -TA-Fe

[0045] a) Add 430 μL TEOS and 8.66 mg TA (doping mass ratio is 8.66%) to the ethanol-water solution with a volume ratio of 60:1, stir for 10 min to mix well; add 0.5 mL ammonia water dropwise, and Reacted at 25°C for 0.5 h to obtain primary SiO containing polyphenol oligomers 2 .

[0046] b) Then add 0.27 mg FeCl 3· 6H 2 O (the molar ratio is TA:Fe=5:1), continue to react in a water bath at 25°C for 5.5 h, after the reaction is completed. The reaction solution was taken out and placed in a centrifuge tube, centrifuged at 10,000 rpm for 10 min, the supernatant was taken out, the precipitate was washed twice with absolute ethanol, and then washed twice with ultrapure water, and the precipitate was dispersed in 10 mL double distilled water to obtain D...

Embodiment 3

[0049] The polyphenol molecules were fixed to ensure that the molar ratio of polyphenols to metal ions was 5:1, and the effects of different doped metal ions on the composite particles were investigated.

[0050] More specifically: SiO 2 -TA-Mn

[0051] a) Add 430 μL TEOS and 12 mg TA (12% doping ratio) into ethanol-water solution with a volume ratio of 60:1, stir for 10 min to mix well; add 0.5 mL of ammonia water dropwise, at 25 After reacting at ℃ for 0.5 h, primary SiO containing polyphenol oligomers was obtained 2 .

[0052] b) Then add 0.28 mg MnCl 2· 4H 2 O (the molar ratio is TA:Mn=5:1), continue to react in a water bath at 25°C for 5.5 h, after the reaction is completed. The reaction solution was taken out and placed in a centrifuge tube, centrifuged at 10,000 rpm for 10 min, the supernatant was taken out, the precipitate was washed twice with absolute ethanol, and then washed twice with ultrapure water, and the precipitate was dispersed in 10 mL double distilled...

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Abstract

The invention belongs to the field of preparation of nanomaterials and particularly relates to a preparation method of a degradable silicon dioxide particle internally doped with a polyphenol-metal mesh. According to the preparation method, a silicon dioxide polycondensation network system is doped with a metal-polyphenol coordinated and complexed network structure, so that porosity of silicon dioxide is increased; and the polycondensation degree of silicon dioxide is further weakened by virtue of a hydrogen-bond interaction between hydroxyl and silanol, so that the hydrolytic degradation of silicon dioxide is accelerated. The metal-polyphenol coordinated and complexed network structure can be dissociated under faintly-acid and high-glutathione environments so as to induce the collapse of a silicon dioxide skeleton structure, nano-particles are degraded into smaller fragments, and then silicic acid molecules, polyphenol molecules and metal ions are further degraded and extracted, so that the self-degradation of a drug carrier in a tumor microenvironment is realized.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation, and in particular relates to a method for preparing silicon dioxide nanoparticles with uniform particle size, monodispersity, good biocompatibility and biodegradability doped with polyphenol-metal mesh. Background technique [0002] As an emerging nanomaterial, nano-silicon dioxide exhibits many impressive characteristics in the medical and biological fields, such as good biological affinity and easy surface modification. However, due to the compact structure of silica, silica particles have quite good thermal stability, and it takes a long time to decompose in cells and living bodies, which can easily cause dose accumulation in tissues and organs, thereby causing DNA damage, Protein denaturation, etc. and lead to a series of bad results. Therefore, the in vivo degradation and clearance of silica nanomaterials using biologically relevant factors has become a research topic worldwide, whic...

Claims

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Application Information

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IPC IPC(8): C01B33/12B82Y40/00A61K47/52A61K31/353A61K31/7024A61P35/00A61P29/00A61P39/06A61K49/08A61K49/00
Inventor 朱春玲郑少秋谢增鸿林旭聪
Owner FUZHOU UNIV
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