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Trichoderma fungus and its agent for degrading organophosphorus pesticides

A technology of organophosphorus pesticides and trichoderma agents, applied in the directions of fungicides, biocides, fungi, etc., can solve the problems of frequent chemical fertilizers and pesticides, microbial ecological imbalance, residues, etc., and achieve high application and promotion value, strong adaptability, The effect of low production cost

Active Publication Date: 2020-05-19
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to perennial planting or continuous cropping, nutrient imbalance, severe pests and diseases, frequent and excessive use of chemical fertilizers and pesticides, soil environment is deteriorating (such as soil secondary salinization, acidification, nutrient imbalance), long-term residues of various pesticides, and microbial ecological imbalance

Method used

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  • Trichoderma fungus and its agent for degrading organophosphorus pesticides
  • Trichoderma fungus and its agent for degrading organophosphorus pesticides
  • Trichoderma fungus and its agent for degrading organophosphorus pesticides

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Preparation of Trichoderma Inoculant for Degrading Organophosphorus Pesticides

[0031] The Trichoderma strain used in the present invention to degrade various organophosphorus pesticides is Trichoderma simmonsii (Trichodermasimmonsii) T21-3, which is isolated and screened from the tomato soil in the laboratory, and is preserved in the China Microbiological Culture Preservation Management Committee Common Microorganisms Center, Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, 100101, the date of deposit is June 24, 2016, and the deposit number is: CGMCC N0.12630. The TEF-1α sequence of the strain was submitted to NCBI BlAST for similarity comparison, and the Trichoderma was identified as Trichoderma simmonsii. The TEF-1α gene sequence has been submitted to the Genbank database with accession number KX426072.

[0032] The preparation of this trichoderma agent comprises the following steps: ...

Embodiment 2

[0039] Preparation of Trichoderma Inoculant for Degrading Organophosphorus Pesticides

[0040] The preparation of this bacterial agent comprises the following steps:

[0041] This implementation case is the same as implementation case 1, the difference is that in step a, the Trichoderma strain is activated and then transferred to the wheat grain solid medium, and placed at room temperature for 7 days of cultivation; in step c, after inoculation, it is also placed Under room temperature (23-28°C) static culture for 10 days, Trichoderma conidia reached 80×10 8 pieces / g; in step e, 75% of diatomaceous earth, 8% of glucose, mixed with 3% of the raw material obtained in step d.

Embodiment 3

[0043] Degradation Test of Trichoderma of the present invention on Organophosphorus Pesticides in Liquid Shaking Flask Conditions

[0044] Step 1: After inoculating the preserved Trichoderma simmonsii T21-3 strain into the PDA plate medium for activation, transfer it to the PDA solid medium in a 500mL Erlenmeyer flask and place it in a constant temperature incubator at 28°C Cultivate in medium for 5 days, wait for it to produce a large amount of conidia, wash with sterile water, dilute, promptly obtain Trichoderma simmons seed liquid (1.0×108CFU / mL);

[0045] Step 2: Add methamidophos, chlorpyrifos, omethoate, parathion, isocarbophos, and phoxim to the sterilized container containing 50 mL of inorganic salts respectively at a concentration of 100 mg / L. In the 250mL Erlenmeyer flask of liquid medium (MSM), inoculate each bacterial strain Trichoderma spore seed liquid (1.0 * 108CFU / mL) 1mL respectively, and described MSM inorganic salt liquid medium is K 2 HPO 4 1.50g, KH 2 ...

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Abstract

The invention discloses a Trichoderma fungus and a bacterial agent thereof for degrading organophosphorus pesticides. The Trichoderma fungus is Trichoderma simmonsii T21-3, which was preserved in the China Committee for Microorganism Culture Preservation on June 24, 2016. General Microorganism Center, deposit number: CGMCC No.12630. The bacterial agent can not only degrade organic phosphorus pesticide residues, but also prevent and control various vegetable soil-borne diseases.

Description

technical field [0001] The invention relates to a trichoderma fungus for degrading organophosphorus pesticides and its bacterial agent, belongs to the field of biotechnology, uses microorganisms to degrade chemical pesticide residues, and is suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. Background technique [0002] Pesticide pollution has become one of the major agricultural eco-environmental issues of widespread concern, posing a serious threat to the sustainable development of modern agriculture and social economy, the safety of the agricultural ecological environment, and the quality and safety of agricultural products. Organophosphorus pesticides, as the main insecticides in domestic and foreign agriculture, kill insects quickly, efficiently, and with low cost. They are widely used in modern agriculture, especially in vegetable cultivation in facilities, and are the main cause of excessive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10A01N63/38A01P3/00C12R1/885
CPCA01N63/30B09C1/105C12N1/14C12N1/145C12R2001/885
Inventor 张广志张新建吴晓青周红姿赵晓燕周方园
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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