New natural product catathelasma imperiale sing polysaccharide CIS-A and application thereof
A natural product, CIS-A technology, applied in drug combination, anti-tumor drugs, allergic diseases, etc., can solve problems such as no reports, and achieve the ability to improve the ability, enhance the ability to phagocytose neutral red, and promote proliferation. Effect
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[0032] Example 1 Extraction, Separation and Purification of Polysaccharide CIS-A
[0033] 1.1. Extraction, separation and purification of polysaccharide CIS-A from Mushroom magnificence
[0034] 1.1.1 Extraction of crude polysaccharides from Mushroom magnifolia
[0035] Place the fruiting body of Mushroom magnificence in a constant temperature box at 65°C to dry, then crush it completely with a pulverizer, weigh 300g of the crushed powder, add distilled water and stir evenly (1:3), cook at 110°C for 6 hours, and centrifuge Collect the supernatant, repeat the cooking three times, and concentrate all the supernatant under reduced pressure, then add 3-4 times the volume of absolute ethanol to precipitate the crude polysaccharides of the Magnificent Pleurotus chinensis. The precipitate was dried and stored in a -20°C refrigerator.
[0036] 1.1.2 Separation and purification of crude polysaccharides from Mushroom magnifolia
[0037] Weigh 50g DEAE-cellulose, soak overnight in dis...
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