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Ag NCs and G-quadruplex/NMM system-based label-free DNA molecular device

A technology of molecular devices and quadruplexes, which is applied in the field of label-free DNA molecular devices, to achieve the effect of selective flexible application and good selectivity

Inactive Publication Date: 2017-11-03
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former may be tolerable in large-scale calculations due to DNA's super-parallel computing capabilities, while the latter may require the introduction of new components

Method used

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  • Ag NCs and G-quadruplex/NMM system-based label-free DNA molecular device
  • Ag NCs and G-quadruplex/NMM system-based label-free DNA molecular device
  • Ag NCs and G-quadruplex/NMM system-based label-free DNA molecular device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Such as figure 1 It is the schematic diagram of the 2-to-1 encoder, figure 2 It is the logic circuit diagram of the 2-to-1 encoder, image 3 is the fluorescence intensity curve of AgNCs at different input values ​​of 2-to-1 encoder and Figure 4 It is the 3D histogram of the normalized value of the fluorescence intensity of AgNCs at different input values ​​of the 2-to-1 encoder:

[0090] Design and determination of a 2-to-1 encoder fluorescence system:

[0091] Construction of 2-to-1 encoder:

[0092] Based on single-stranded P-DNA, prepare P-DNA (10μL, 100μmol / L), Mg(AC) 2 (10μL, 100mmol / L) and PBS buffer (950μL, 20mmol / L, PH=7.00) solution, divided into:

[0093] Add P0-DNA (10 μL, 100 μmol / L) to the solution of group a;

[0094] Add P1-DNA (10 μL, 100 μmol / L) to the solution of group b;

[0095] Add AgNO after incubation at 37°C for half an hour 3 (10μL, 0.6mmol / L), magnetically stirred for 2 minutes, incubated at 4°C in the dark for 30 minutes, and quickly...

Embodiment 2

[0103] Such as Figure 5 is the schematic diagram of the 4-to-2 encoder, Figure 6 It is the logic circuit diagram of the 4-to-2 encoder, Figure 7 is the fluorescence intensity curve of AgNCs and NMM at different input values ​​of 4-to-2 encoder and Figure 8 It is the 3D histogram of the normalized value of the fluorescence intensity of AgNCs and NMM at different input values ​​of the 4-to-2 encoder:

[0104] Design and measurement of 4-to-2 encoder fluorescence system:

[0105] Based on single-stranded HS-DNA, the

[0106] a sample solution: P0-DNA (10 μL, 100 μmol / L) solution + HS-DNA (10 μL, 100 μmol / L)) solution;

[0107] b sample solution P1-DNA (10 μL, 100 μmol / L) + HS-DNA (10 μL, 100 μmol / L)) solution;

[0108] c sample solution P2-DNA (10 μL, 100 μmol / L) + HS-DNA (10 μL, 100 μmol / L)) solution;

[0109] d sample solution P3-DNA (10 μL, 100 μmol / L) solution + HS-DNA (10 μL, 100 μmol / L)) solution;

[0110] Add Mg(AC) to the four sample solutions of the above solu...

Embodiment 3

[0126] Such as Figure 9 It is the schematic diagram of the 1-to-2 decoder, Figure 10 is the fluorescence intensity curve of AgNCs and NMM at different input values ​​of the 1-to-2 decoder and Figure 11 It is the 3D histogram of the normalized value of the fluorescence intensity of AgNCs and NMM at different input values ​​of the 1-to-2 decoder:

[0127] Design and determination of 1-to-2 decoder fluorescence system:

[0128] Ag NCs formed with P / StrB1 and NMM / K with strong emission at 570nm + The system is based on:

[0129] In group a test tube: add P-DNA (10 μL, 100 μmol / L), StrB1-DNA (10 μL, 100 μmol / L), Mg(AC) 2 (10μL, 100mmol / L) and PBS buffer solution (950μL, PH=7.00), add ultrapure water (10μL);

[0130] b Two groups of test tubes: add P-DNA (10 μL, 100 μmmol / L), StrB1-DNA (10 μL, 100 μmmol / L), Mg(AC) 2 (10μL, 100mmol / L) and PBS buffer solution (950μL, PH=7.00), add StrB2-DNA (10μL, 100μmmol / L);

[0131] Add AgNO respectively after incubating at 37°C for 30 mi...

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Abstract

The invention discloses an Ag NCs and G-quadruplex / NMM system-based label-free DNA molecular device. According to the device, a nano-silver cluster synthesized by taking a dsDNA-loop-C6 structure as a template is constructed; through constructing two G4-quadruplex structure / NMM fluorescent probes, fluorescent outputs of yellow and red are formed; a double-output molecular logic gate is constructed; yellow and red fluorescence intensities are taken as judging basis; and when the normalized numerical values of the fluorescence intensities are greater than 0.45, the outputs are 1, and otherwise, the outputs are 0. The inputs of different DNA chains generate different influences on the two fluorescent probes, and different fluorescent signals are output. On the basis of above principle, five DNA molecular logic gates such as a 2-to-1 coder, a 4-to-2 coder, a 1-to-2 decoder, a half adder and a half subtractor are constructed, and the DNA molecular logic gates can be used for detecting different DNA sequences.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular computer, and discloses a label-free DNA molecular device based on Ag NCs and G-quadruplex / NMM system, the construction of the DNA molecular device and the application of the DNA molecular device by means of fluorescence. Background technique [0002] In 1946, the first computer DNIAC was put into operation at the University of Pennsylvania in the United States. It has developed rapidly in the past 50 years and has had a huge impact on people's production and lifestyle in the 21st century. Although the computing speed and storage capacity of computers were at the top of the list at that time, with the continuous improvement of human demand for computer technology, traditional crystalline silicon chips faced the problem of "Moore's Law". In order to overcome this problem, scientists have proposed the idea of ​​molecular devices. Compared with traditional semiconductor electronic devices, mol...

Claims

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Application Information

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IPC IPC(8): G06F19/20H03K19/20
CPCG16B25/00H03K19/20
Inventor 吕晓艳胡晓春魏伊彤石硕姚天明
Owner TONGJI UNIV
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