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A method for detecting the content of organic synthetic pigments

A detection method and organic synthesis technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult content determination, difficult extraction, inability to measure sodium aluminum lake of indigo disulfonate, etc.

Active Publication Date: 2019-12-10
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because allura red aluminum lake and indigo sodium disulfonate aluminum lake are neither soluble in water nor in organic solvents, it is difficult to extract allura red and indigo sodium disulfonate from the sample, resulting in great difficulty in content determination
Therefore, none of the existing reported methods can determine sodium indigo disulfonate aluminum lake and allura red aluminum lake in food.

Method used

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  • A method for detecting the content of organic synthetic pigments
  • A method for detecting the content of organic synthetic pigments
  • A method for detecting the content of organic synthetic pigments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1 Principle: Allura red and indigo sodium disulfonate in the sample are extracted with sodium hydroxide solution, and after neutralization with hydrochloric acid solution, allura red and indigo sodium disulfonate are separated by high performance liquid chromatography C18 column, and separated by diode array Detector detection, and quantification by external standard method.

[0067] 2 Instrument: Agilent 1260 high performance liquid chromatography (with diode array detector), or other high performance liquid chromatography with diode array detector.

[0068] 3 Reagents Reagents: sodium hydroxide (AR, ie analytical grade); concentrated hydrochloric acid (AR); ammonium acetate (chromatographically pure); acetonitrile (chromatographically pure); primary water.

[0069] Allura Red, reference substance source: China National Institutes for Food and Drug Control, batch number: 510003-201301, purity: 86.7%;

[0070] Sodium indigo disulfonate, reference source: SIGMA, batch n...

Embodiment 2

[0160] 1 Analysis steps:

[0161] 1.1 Chromatographic conditions:

[0162] Mobile phase: A: 30mmol / L ammonium acetate solution, B: acetonitrile, gradient elution;

[0163] Flow rate: 0.8mL / min;

[0164] Wavelength: Allure Red (500nm), Sodium Indigo Disulfonate (600nm);

[0165] Column temperature: 30°C;

[0166] Injection volume: 5 μL;

[0167] Chromatographic column: PHenomenex Gemini-NX, C18, 250×4.6 mm, 5 μm.

[0168] 1.2 Preparation of control stock solution:

[0169] Accurately weigh 5.7mg of Allura Red reference substance, put it in a 50mL brown volumetric flask, add water to dissolve it and adjust the volume to the mark, and shake well to obtain the stock solution. The stock solution was stored in a refrigerator at 4°C for future use.

[0170] Precisely weigh 8.3mg of indigo sodium disulfonate reference substance, put it in a 50mL brown volumetric flask, add water to dissolve it, adjust the volume to the mark, and shake well to obtain the stock solution. The sto...

Embodiment 3

[0185] 1 Analysis steps:

[0186] 1.1 Chromatographic conditions:

[0187] Mobile phase: A: 100mmol / L ammonium acetate solution, B: acetonitrile, gradient elution;

[0188] Flow rate: 0.5mL / min;

[0189] Wavelength: Allure Red (500nm), Sodium Indigo Disulfonate (600nm);

[0190] Column temperature: 35°C;

[0191] Injection volume: 10μL;

[0192] Chromatographic column: PHenomenex Gemini-NX, C18, 250×4.6 mm, 5 μm.

[0193] 1.2 Preparation of control stock solution:

[0194] Accurately weigh 5.7mg of Allura Red reference substance, put it in a 50mL brown volumetric flask, add water to dissolve it and adjust the volume to the mark, and shake well to obtain the stock solution. The stock solution was stored in a refrigerator at 4°C for future use.

[0195] Precisely weigh 8.3mg of indigo sodium disulfonate reference substance, put it in a 50mL brown volumetric flask, add water to dissolve it, adjust the volume to the mark, and shake well to obtain the stock solution. The st...

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Abstract

The invention relates to the technical field of quality control and especially relates to a detection method for organic artificial color content. The detection method comprises the following steps: mixing a sample with alkali liquor and regulating pH value to 7, thereby acquiring a tested liquor, and then adopting high performance liquid chromatography for detecting the tested liquor and acquiring the organic artificial color content according to a content detection result of a standard substance. The chromatographic conditions of the high performance liquid chromatography are as follows: flowing phases: ammonium acetate is served as a flowing phase A and acetonitrile is served as a flowing phase B; elution process: gradient elution; chromatographic column: C18 chromatographic column; enticed red and / or sodium indigotin-disulfonate is served as the organic artificial color. The linearization, precision, repeatability, stability, blank, detection limit, quantitation limit and recovery rate of detection method provided by the invention all meet the requirement of GB / T27404-2008 'Laboratory Quality Control Specification'; the content detection method is proved as scientific and effective; the purpose of quality control for the content of enticed red and / or sodium indigotin-disulfonate in a tablet can be achieved.

Description

technical field [0001] The invention relates to the technical field of quality control, in particular to a method for detecting the content of organic synthetic pigments. Background technique [0002] Synthetic pigments are artificially synthesized pigments, which have many advantages, such as bright color, strong tinting strength, and various hues, which can improve the appearance of products and attract consumers to buy them, so they are widely used in the food field. China allows a total of 28 types of synthetic pigments to be added to food. The classification of synthetic pigments includes organic synthetic pigments, inorganic synthetic pigments, and natural equivalent synthetic pigments. Among them, organic synthetic pigments usually include: amaranth, carmine, tartrazine, new red, erythrosine, allura red, sunset yellow, brilliant blue and indigo (in the food industry, indigo is known as its sodium sulfonate or its aluminum It can be used as food coloring in the form o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 苏昭仑叶少文游景水
Owner BY HEALTH CO LTD
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