A kind of detection method of xylo-oligosaccharide content
A detection method and technology of xylo-oligosaccharide, applied in the field of analytical chemistry, to achieve the effects of system stability, good reproducibility and low consumption
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Embodiment 1~3
[0051] The detection of embodiment 1~3 xylo-oligosaccharide content
[0052] Liquid chromatography conditions:
[0053] The chromatographic condition of table 1 embodiment 1~3 of the present invention
[0054]
[0055] 2. Preparation of reference substance stock solution:
[0056] Weigh 50 mg of xylose reference substance, add purified water to ultrasonically dissolve it, then dilute it to a 10 mL volumetric flask with purified water to prepare a standard stock solution with a concentration of 5.0 mg / mL, and store it at 4°C in the dark.
[0057] 3. Standard working curve drawing:
[0058] Take the above-mentioned standard stock solution 0.04mL, 0.1mL, 0.2mL, 0.4mL, 0.8mL into a 10mL volumetric flask, dilute to a concentration of 0.02mg / mL, 0.05mg / mL, 0.10mg / mL, 0.20mg / mL, 0.40mg / mL standard test solution, concentration from low to high were injected into the test and draw the working curve.
[0059] 4. Sample preparation:
[0060] Accurately weigh 650±50mg of the sampl...
Embodiment 1
[0086] Table 2 Example 1 xylo-oligosaccharide content detection result
[0087]
[0088] Note: The theoretical value of xylooligosaccharides in Qingchang powder is 8.10%.
Embodiment 4
[0089] Embodiment 4 linear range confirmation
[0090] Obtain the experimental data of standard product according to embodiment 1 detection method, see table 3:
[0091] Table 3 standard test data
[0092]
[0093] With the concentration C as the ordinate and the peak area A as the abscissa, draw a standard working curve, such as Figure 4 shown.
[0094] Linear evaluation: correlation coefficient R 2 It is 0.999, so this method is used to indirectly measure the content of xylo-oligosaccharides, showing good linearity between the concentration of 0.0378mg / mL and 0.4536mg / mL, which is in line with GB / T27404-2008 "Laboratory Quality Control Standards for Food Physical and Chemical Testing "Requirements [GB / T 27404-2008 requires correlation coefficient R 2 ≥0.99].
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