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Rhyronic acid i derived from Penicillium oxalicum and its application as an anti-human oral epidermoid cancer drug

A technology of oral epidermoid carcinoma and ryeconic acid, applied in the field of medicine and biology

Active Publication Date: 2020-05-08
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the indicated ryronic acid compounds have anti-human oral epidermoid cancer activity, and there is no report on the anti-proliferation activity of this compound on human oral epidermoid cancer cells, so there are no related drugs on the market.

Method used

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  • Rhyronic acid i derived from Penicillium oxalicum and its application as an anti-human oral epidermoid cancer drug
  • Rhyronic acid i derived from Penicillium oxalicum and its application as an anti-human oral epidermoid cancer drug
  • Rhyronic acid i derived from Penicillium oxalicum and its application as an anti-human oral epidermoid cancer drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Fermentative production and separation and purification of the compound of embodiment 1

[0020] 1 Fermentation production

[0021] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium oxalicum ( Penicillium oxalicum ) IBPT-6 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013714) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 2 to 3 days.

[0022] Penicillium oxalicum ( Penicillium oxalicum ) Appropriate amount of IBPT-6, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 ·7H 2 O 0.3, NaCl 15.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermentation broth ...

Embodiment 2

[0031] Example 2 Test of Anti-tumor Activity in Vitro

[0032] 1 Experimental samples and experimental methods

[0033] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0034] The subculture of cell lines and cells adopts tumor cell lines, and the tumor cells are treated with DMEM medium containing 10% FBS at 37°C with 5% CO 2 Subculture in an incubator.

[0035] Cell Proliferation Inhibitory Activity Test Method

[0036] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 1×10 per ml5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2 incubator for 4 hours. Add 2 microliters of sample solution or bla...

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Abstract

The invention relates to secalonic acid I derived from penicillium oxalicum and application of the secalonic acid I for preparing a medicine for preventing human oral epidermoid carcinoma. The compound has a function of inhibiting human oral epidermoid carcinoma cell proliferation. The structural formula of the secalonic acid I is as shown in the specification. Through fermentation culture of Penicillium oxalicum IBPT-6, a fermented substance is prepared, and the compound is separated and purified from the fermented substance. Tests show that the compound has relatively high anti-tumor activity upon human oral epidermoid carcinoma cells KB. The compound can be used for preventing human oral epidermoid carcinoma cell proliferation inhibition medicines or anti-tumor medicines for studying human oral epidermoid carcinoma.

Description

technical field [0001] The invention relates to the application of rykonic acid I derived from Penicillium oxalicum and an anti-human oral epidermoid cancer drug, and belongs to the field of medicine and biology. Background technique [0002] Secalonic acids belong to the secondary metabolites of Ergochrome and are xanthone dimers. Since Stoll et al. isolated Secalonic acid A (Secalonic acid A) from fungi in 1952, the series of compounds Secalonic acid (A, B, C, D, E, F, G) have been continuously identified. discovery and research. Secalonic acid compounds have various physiological activities. Taking Secalonic acid D (Secalonic acidD, SAD) as an example, 5 mg / ml of SAD is added to normal saline, within the range of 5-20 mg, that is It can treat early bladder cancer, within the range of 50-100 mg, it is effective for more serious bladder cancer and has no side effects. It has been found through research that some marine fungi can produce ryryronic acid compounds with nove...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/86C12P17/06A61P35/00C12R1/80
CPCC07D311/86C12P17/06
Inventor 陈立王思远夏其文刘沁颖毕延雪伍久林张其清
Owner FUZHOU UNIV
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