A system and method for constructing a three-dimensional micro-nano-scale prefabricated vascular network of bone tissue

A technology of micro-nano scale and construction method, applied in tissue regeneration, processing and manufacturing, manufacturing tools, etc., can solve problems such as can not be effectively solved, and achieve the effect of easy material, material acquisition, and simple preparation process

Active Publication Date: 2020-10-30
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this method cannot effectively solve the problem of prefabricated vascularized network of bone tissue at the micro-nano scale

Method used

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  • A system and method for constructing a three-dimensional micro-nano-scale prefabricated vascular network of bone tissue
  • A system and method for constructing a three-dimensional micro-nano-scale prefabricated vascular network of bone tissue
  • A system and method for constructing a three-dimensional micro-nano-scale prefabricated vascular network of bone tissue

Examples

Experimental program
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Embodiment 1

[0031] In this embodiment, see Figure 1 ~ Figure 3 , A method for constructing a three-dimensional micro-nano-scale prefabricated blood vessel network of bone tissue, including the following steps:

[0032] a. Use a blend of PVA aqueous solution and chitosan solution as the sacrificial material, and use anhydrous ethanol as the initiating agent and the coordinated movement of the aluminum alloy shaft as the receiving device, and connect the positive electrode of the high-voltage power supply to the 3D printing nozzle. The wire is connected with the rotating receiving shaft. When there is voltage, the fiber filament formed connects the nozzle with the rotating receiving shaft to form a closed path. The direct writing process can be controlled by shortening the distance between the nozzle and the rotating receiving shaft;

[0033] b. Replace the nozzle in step a with a 20G needle, enter the coding program of the sacrificial structure in the 3D printer and run it. Under the action of...

Embodiment 2

[0038] This embodiment is basically the same as the first embodiment, and the special features are:

[0039] In this embodiment, a method for constructing a three-dimensional micro-nano-scale prefabricated blood vessel network of bone tissue, using PGA as the polymer I material, includes the following steps:

[0040] a. Use a blend of PGA aqueous solution and chitosan solution as the sacrificial material, and use anhydrous ethanol as the initiating agent and a coordinated movement aluminum alloy shaft as the receiving device. Connect the positive electrode of the high-voltage power supply to the 3D printing nozzle. The wire is connected with the rotating receiving shaft. When there is voltage, the fiber filament formed connects the nozzle with the rotating receiving shaft to form a closed path. The direct writing process can be controlled by shortening the distance between the nozzle and the rotating receiving shaft;

[0041] b. Replace the nozzle in step a with a 20G needle, enter t...

Embodiment 3

[0045] This embodiment is basically the same as the foregoing embodiment 1, and the special features are:

[0046] In this embodiment, a method for constructing a three-dimensional micro-nano-scale prefabricated vascular network of bone tissue, the hydrogel adopts gelatin loaded with human bone marrow mesenchymal stem cells, and includes the following steps:

[0047] a. This step is the same as in Example 1;

[0048] b. This step is the same as in Example 1;

[0049] c. After the three-dimensional micro-nano-scale sacrificial module prepared in step b is formed, a layer of liquid paraffin is coated on its surface, and then placed in a 37°C drying oven to dry the paraffin, while 15% gelatin is prepared with deionized water, Place in a 60°C water bath and stir until the gelatin particles are uniformly dissolved and the temperature drops to 30°C. At this time, add mTG enzyme (the mass ratio of gelatin to mTG enzyme is 10:1) to crosslink the gelatin solution. Take out the fifth-generatio...

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Abstract

The invention discloses a construction system and method of a 3D (three-dimensional) micro / nano-scale prefabricated vessel network of bone tissues, which are used for the field of bio-manufacturing and used for manufacturing a micro / nano-scale prefabricated vessel access structure by combining an electrohydrodynamics direct-writing process and a subtractive manufacturing technology. A 3D shape of a required sacrificial material is prepared by promoting the sacrificial material to form sacrificial material solution with anhydrous ethanol, no harmful substance is generated in the whole process, and the material is easily available. The micro / nano-scale vessel structure is formed by virtue of printability of PVA (Polyvinyl Acetate), PGA (propylene glycol alginate) and chitosan, and the problem that the 3D micro / nano-scale vessel network cannot be obtained through biological 3D printing is solved, therefore, the system and the method have significance for solving the problem of the scale of a vessel in repair of human tissues in clinical medicine.

Description

Technical field [0001] The invention relates to a system and method for constructing a bone tissue three-dimensional micro-nano-scale prefabricated blood vessel network, which is applied to the technical field of biological manufacturing. Background technique [0002] The ultimate goal of tissue engineering is to implant artificial tissues or organs to replace the diseased parts of the human body, and to provide different solutions for tissue or organ transplantation to improve the quality of life and prolong life activities. Biological 3D printing technology has made great progress in regenerative tissue repair, and a large number of biological scaffolds have been prepared for clinical applications, such as skin, bones, cartilage and nerves. However, there are still some technical obstacles to overcome. One of them is to construct a three-dimensional micro-nano-scale prefabricated blood vessel network, especially for smaller tissues and organs. The vascular network plays a role...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/22A61L27/38A61L27/20A61L27/16A61L27/18A61L27/50B29C64/106B29C64/20B29C64/40B33Y10/00B33Y30/00B33Y70/10
CPCA61L27/16A61L27/18A61L27/20A61L27/222A61L27/3834A61L27/50A61L27/52A61L2400/12A61L2430/02B33Y10/00B33Y30/00B33Y70/00C08L5/08C08L29/04C08L67/04
Inventor 胡庆夕孙程艳刘媛媛李帅李冬冬
Owner SHANGHAI UNIV
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