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Enzymatic degumming process based on immobilized compound phospholipase

A technology of compound phospholipids and phospholipases, which is applied in the production of fat, fat oil/fat refining, etc., can solve the problems of long degumming time, PLA and PLC residues, and cannot be reused, and achieves improved degumming effect, high degumming efficiency, and improved efficiency. rate effect

Inactive Publication Date: 2017-10-20
ANHUI LEVEKING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the co-degumming of PLA and PLC can greatly increase the degumming speed, PLA and PLC will remain in the oil and cannot be reused
In enzymatic degumming, in order to reuse phospholipase, Chinese patent CN105132147 discloses a method for degumming soybean oil with immobilized PLC, which solves the problems of one-time use of PLC and difficulty in product separation and purification, but still uses Single phospholipase, long degumming time; Chinese patent CN104560387 discloses an immobilized PLA 1 Combined with immobilized PLC to remove crude soybean oil, after using 6 batches, the relative activity of the immobilized enzyme remained at about 80%, but the PLA in this method 1 It is not immobilized on the same carrier at the same time as PLC, but it is actually immobilized PLA 1 and immobilized PLC acted separately for degumming, and did not play the role of PLA 1 Synergy with PLC; Chinese patent CN102776066 discloses a method using immobilized PLA 2 The method of degumming, the activity of the immobilized enzyme can keep more than 54% after repeated use 7 times, the same is the use of a single phospholipase, the degumming time is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The immobilized composite phospholipase was prepared with macroporous resin D101.

[0027] 1. Pretreatment of macroporous resin

[0028] The pretreatment method of macroporous resin D101 for adsorption is:

[0029] (1) Soak in NaCl solution with a mass fraction of 10% for 4 hours, wash with water and filter with suction;

[0030] (2) Soak in ethanol with a volume fraction of 95% for 4 h, wash with water and filter with suction;

[0031] (3) Soak in HCl solution with a mass fraction of 5% for 4 hours, wash with water and filter until neutral;

[0032] (4) Soak in NaOH solution with a mass fraction of 2% for 4 hours, wash with water and filter until neutral;

[0033] (5) Soak in deionized water and store in a 4°C refrigerator for later use.

[0034] Second, the preparation of the original enzyme solution

[0035] Weigh PLA respectively 1 , PLC, L03 lipase and protease 8 g , 8 g , 2 g and 2 g were extracted with 100 mL glycine-sodium hydroxide buffer solution with pH 9...

Embodiment 2

[0040] The immobilized composite phospholipase was prepared with macroporous resin AB-8.

[0041] 1. Pretreatment of macroporous resin

[0042] The pretreatment method of macroporous resin AB-8 for adsorption is:

[0043] (1) Soak in NaCl solution with a mass fraction of 10% for 4 hours, wash with water and filter with suction;

[0044] (2) Soak in ethanol with a volume fraction of 95% for 4 h, wash with water and filter with suction;

[0045] (3) Soak in HCl solution with a mass fraction of 5% for 4 hours, wash with water and filter until neutral;

[0046] (4) Soak in NaOH solution with a mass fraction of 2% for 4 hours, wash with water and filter until neutral;

[0047] (5) Soak in deionized water and store in a 4°C refrigerator for later use.

[0048] Second, the preparation of the original enzyme solution

[0049] Weigh PLA respectively 1 , PLC, L03 lipase and protease 8 g , 8 g , 2 g and 2 g were extracted with 100 mL glycine-sodium hydroxide buffer solution with pH...

Embodiment 3

[0054] The immobilized composite phospholipase was prepared with macroporous resin D3520.

[0055] 1. Pretreatment of macroporous resin

[0056] The pretreatment method of macroporous resin D3520 for adsorption is:

[0057] (1) Soak in NaCl solution with a mass fraction of 10% for 4 hours, wash with water and filter with suction;

[0058] (2) Soak in ethanol with a volume fraction of 95% for 4 h, wash with water and filter with suction;

[0059] (3) Soak in HCl solution with a mass fraction of 5% for 4 hours, wash with water and filter until neutral;

[0060] (4) Soak in NaOH solution with a mass fraction of 2% for 4 hours, wash with water and filter until neutral;

[0061] (5) Soak in deionized water and store in a 4°C refrigerator for later use.

[0062] Second, the preparation of the original enzyme solution

[0063] Weigh PLA respectively 1 , PLC, L03 lipase and protease 10 g , 10 g , 2 g and 2 g were extracted with 100 mL glycine-sodium hydroxide buffer solution of ...

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Abstract

The invention discloses an enzymatic degumming process based on immobilized compound phospholipase. The enzymatic degumming process comprises the following steps: A, preparing the immobilized compound phospholipase; B, applying the immobilized compound phospholipase to degumming of oils and fats, wherein the compound phospholipase contains phospholipase A1, phospholipase C, lipase and protease. According to the enzymatic degumming process based on the immobilized compound phospholipase, the phospholipase A1, the phospholipase C, the lipase and the protease which are used for degumming are immobilized on the same macroporous resin simultaneously firstly, then the macroporous resin immobilized with the phospholipase A1, the phospholipase C, the lipase and the protease is used for degumming the oils and fats, the phospholipase A1, the phospholipase C, the lipase and the protease play a synergistic role to degum the oils and fats, so that the immobilized compound phospholipase can be used repeatedly for degumming, enzyme residues are avoided in the oils and fats, the degumming efficiency is high, and oil yield is high.

Description

technical field [0001] The invention relates to the technical field of enzymatic degumming of grease, in particular to a process for enzymatic degumming of grease by using immobilized compound phospholipase. Background technique [0002] Crude oil extracted from oil seeds by pressing, leaching, etc. contains impurities such as proteins, sugars, phospholipids, pigments, metal ions, free fatty acids, etc., and is usually not edible directly. Only when the impurities are removed can the edible oil that meets the national requirements be obtained. The phospholipids, proteins and mucous substances contained in crude oil are collectively called colloids, and the colloids in crude oil are mainly phospholipids, so degumming is also called dephosphorization. To meet the requirements of physical refining, the phosphorus content of oil must be controlled within 10mg / kg during the degumming process, otherwise the phospholipids will easily coke at high temperature after entering the deo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00
CPCC11B3/003
Inventor 郭宏涛王剑英贾佳
Owner ANHUI LEVEKING BIOTECH CO LTD
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