Method for removing residuals of sarpogrelate hydrochloride in high-performance liquid chromatography sample injection valve

Abstract

The invention discloses a method for removing residuals of sarpogrelate hydrochloride in a high-performance liquid chromatography sample injection valve. The sample injection valve is cleaned through multiple times of sample injection of a blank solvent, wherein the blank solvent is a N-methyl pyrrolidone aqueous solution with the volume percent fraction of 50%-70%. Sarpogrelate hydrochloride is a 5-HT2 receptor blocker, can inhibit platelet aggregation and vasoconstriction and has an anti-thrombotic effect and a microcirculation improving effect. The compound is very liable to remain in the sample injection valve although the water solubility is very good, then a small peak can occur at the sarpogrelate peak appearance position even though the blank solvent is injected, a quantitation limit and detection limit determination experiment of sarpogrelate hydrochloride can not be carried out at all due to the phenomenon, and the sarpogrelate hydrochloride content determination accuracy is influenced. According to the method, the efficiency of removing the sarpogrelate hydrochloride residues in the sample injection valve is high, and the working efficiency can be improved.

Description

technical field [0001] The invention belongs to the field of instrument analysis, and relates to a method for cleaning sarcogrelate hydrochloride residue in a high-efficiency liquid phase sampling valve. Background technique [0002] In the field of drug analysis, some components in the test solution are likely to remain in the HPLC injection valve, resulting in "ghost peaks" in the liquid chromatogram, affecting the normal analysis of samples. When this happens, usually use 50% isopropanol aqueous solution as a blank solvent, and repeatedly inject samples to clean the injection valve; if this method still cannot effectively remove the residue, you need to remove the injection valve and use 50% isopropanol to clean the injection valve. Ultrasonic cleaning in aqueous solution, but this method is not recommended, because repeated disassembly will affect the accuracy of the injection. [0003] Sagrelate hydrochloride is a 5-HT2 receptor blocker, which can inhibit platelet aggr...

AI Technical Summary

Problems solved by technology

Although this compound has good water solubility, it is very easy to remain in the injection valve, causing a small peak to appear at the peak of sagrelate even when injecting a blank solvent. Limit determination experiment can not be carried out at all, but also will affect the accuracy of sarcogrelate hydrochloride content determination

The inventor used 50% isopropanol aqueous solution as the blank solvent, injected 100 μL each time, and needed more than 300 repeated injections to clean it, and the number of repeated injections was too many, and the cleaning efficiency was low

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