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Immunopotentiator for swine atrophic rhinitis inactivated vaccine and preparation method of immunopotentiator

An immune enhancer and technology for atrophic rhinitis, which is applied in the field of immune enhancer for porcine atrophic rhinitis inactivated vaccine and its preparation, can solve the problems of long duration of immunity, expensive raw materials, high cost, etc., and achieve the extension of duration of immunity , avoid latent infection, inhibit the effect of latent infection

Active Publication Date: 2017-10-20
SHANGHAI CHUANG HONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adjuvants of porcine atrophic rhinitis inactivated vaccine currently on the market include aluminum hydroxide gel, lipid adjuvant and propolis adjuvant; aluminum hydroxide gel adjuvant has good immunogenicity and small side effects, but it cannot induce cellular immunity ; Lipid adjuvant and propolis adjuvant have good immunogenicity, can induce humoral immunity and cellular immunity, and have a long duration of immunity, but the raw materials are expensive and the cost is too high
Therefore, in recent years, researchers from various countries have developed a series of new adjuvants using different raw materials and methods, but none of them have achieved satisfactory results due to poor effects or high toxicity.

Method used

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  • Immunopotentiator for swine atrophic rhinitis inactivated vaccine and preparation method of immunopotentiator
  • Immunopotentiator for swine atrophic rhinitis inactivated vaccine and preparation method of immunopotentiator
  • Immunopotentiator for swine atrophic rhinitis inactivated vaccine and preparation method of immunopotentiator

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a. Liquid preparation containing dry powder immune particles: take 30ml of an aqueous solution with a concentration of 100ug / ml of aluminum hydroxide, add 4ug of ginsenoside, 4ug of cholesterol, and 4ug of diethylaminoethyl dextran under slow stirring, and continue stirring Add polyethylene glycol after 5 minutes, the total moles of vitamin C and potassium iodide / moles of aluminum hydroxide in the liquid containing dry powder immune particles=4, and the mass ratio of aluminum hydroxide to polyethylene glycol is 1:1 After continuing to stir for 20 minutes, a golden liquid containing dry immune particles was obtained. Observing with an atomic microscope, the obtained dry powder immune particles had a diameter of 50 nm. The liquid containing dry powder immune particles was packaged in a brown collection bottle and filled with liquid nitrogen. Do aseptic storage, the shelf life is 2 years;

[0023] b. Preparation of immune enhancer: take a suitable amount of liquid containi...

Embodiment 2

[0025] a. Liquid preparation containing dry powder immune particles: Take 30ml of an aqueous solution with a concentration of 100ug / ml of aluminum hydroxide, add 80ug of ginsenoside, 80ug of cholesterol, and 40ug of diethylaminoethyl dextran under slow stirring, and continue stirring Add polyethylene glycol after 3 minutes, the total moles of vitamin C and potassium iodide in the liquid containing dry immune particles / moles of aluminum hydroxide = 1, and the mass ratio of aluminum hydroxide to polyethylene glycol is 1:0.1 , and continue to stir for 10 minutes to obtain a golden liquid containing dry immune particles. Observing with an atomic microscope, the diameter of the obtained dry immune particles is 120nm. The liquid containing dry immune particles is encapsulated in a brown collection bottle and filled with liquid nitrogen. Do aseptic storage, the shelf life is 2 years;

[0026] b. Preparation of immune enhancer: take an appropriate amount of liquid containing dry powde...

Embodiment 3

[0028] a. Liquid preparation containing dry powder immune particles: take 30ml of an aqueous solution with a concentration of 100ug / ml of aluminum hydroxide, add 40ug of ginsenoside, 40ug of cholesterol and 20ug of diethylaminoethyl dextran under slow stirring, and continue stirring for 4min After adding polyethylene glycol, the total moles of vitamin C and potassium iodide in the liquid containing dry powder immune particles / moles of aluminum hydroxide = 2, and the mass ratio of aluminum hydroxide to polyethylene glycol is 1:0.5, After continuing to stir for 15 minutes, golden yellow liquid containing dry immune particles was obtained. Observing with an atomic microscope, the obtained dry powder immune particles had a diameter of 100nm. The liquid containing dry powder immune particles was encapsulated in a brown collection bottle, filled with liquid nitrogen to make Aseptically stored, the shelf life is 2 years;

[0029] b. Preparation of immune enhancer: take an appropriate...

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Abstract

The invention discloses an immunopotentiator for a swine atrophic rhinitis inactivated vaccine. The immunopotentiator comprises a liquid containing dry powder immunity particles, and a solvent, wherein the liquid containing the dry powder immunity particles mainly comprises an aluminium hydroxide aqueous solution, ginsenoside, cholesterol, a vitamin C, diethylaminoethyl glucan, potassium iodide and polyethylene glycol according to an appropriate ratio by compatibility. Compared with the prior art, the prepared immunopotentiator for the swine atrophic rhinitis inactivated vaccine can effectively inhibit establishment and reactivation of latent infection of bordetella bronchiseptica and pasteurella multocida, can shorten a window phase of antibody production, improves an immunity effect of the swine atrophic rhinitis vaccine, prolongs the immunity duration, effectively avoids latent infection of swine atrophic rhinitis, provides guarantee for prevention and elimination of the swine atrophic rhinitis, can be widely applied to a pig farm for prevention and treatment of the swine atrophic rhinitis, and increases an economic benefit of the pig farm.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an immune enhancer for porcine atrophic rhinitis inactivated vaccine and a preparation method thereof. Background technique [0002] Porcine atrophic rhinitis is a chronic and contagious respiratory disease mainly caused by Bordetella bronchiseptica and toxigenic Pasteurella multocida. The World Health Organization defines it as a Class B disease. The sick pigs have adverse reactions such as sneezing, nosebleeds, facial deformation, nose skewing, and growth retardation, which lead to a decrease in the feed conversion rate of the pigs and low production performance. Pig farming has caused huge economic losses. At the same time, after the pathogen infects the pig, it will damage the normal structure and function of the respiratory tract, reduce the pig's body resistance, and easily infect other pathogens, cause respiratory syndrome, and increase ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/116A61K39/102A61K39/02A61P31/04A61P37/04
CPCA61K39/0208A61K39/102A61K39/39A61K2039/521A61K2039/552A61K2039/55505A61K2039/55511A61K2039/55577A61K2039/55583A61K2039/57A61K2039/575
Inventor 马晶晶赖志吴碧清高俊锋韩相敏张秉金沈明志张瑜
Owner SHANGHAI CHUANG HONG BIOTECH
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