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Preparation methods and applications of high-purity total iridoid glycoside extract from folium syringae and preparations of high-purity total iridoid glycoside extract from folium syringae

A total iridoid glycosides, high-purity technology, applied in the direction of pill delivery, pharmaceutical formulations, antiviral agents, etc., to achieve the effects of short filling time, good application prospects, and large sample load

Inactive Publication Date: 2017-10-20
HARBIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the widespread use of antibiotics, the problem of bacterial resistance has become increasingly prominent

Method used

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  • Preparation methods and applications of high-purity total iridoid glycoside extract from folium syringae and preparations of high-purity total iridoid glycoside extract from folium syringae
  • Preparation methods and applications of high-purity total iridoid glycoside extract from folium syringae and preparations of high-purity total iridoid glycoside extract from folium syringae
  • Preparation methods and applications of high-purity total iridoid glycoside extract from folium syringae and preparations of high-purity total iridoid glycoside extract from folium syringae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 High-purity clove leaves total iridoid glycosides extract was prepared by dynamic ultrasonic countercurrent extraction combined with macroporous adsorption resin column chromatography and dynamic axial compression industrial preparation chromatographic separation technology:

[0020] Grind 10 kg of clove leaves to 20-30 meshes, add 8 times the amount of ethanol with a concentration of 70% by volume, extract continuously at a frequency of 30 kHz and ultrasonic power of 20 kW for 3 times, 40 min each time, filter, and combine the extracts. Recover the extraction solvent under reduced pressure, properly concentrate to a concentration of 0.6 g crude drug / ml, add 1 mol / L hydrochloric acid to adjust the pH value to 4, and pass through the HPD650 macroporous adsorption resin chromatography column at a flow rate of 2 BV / h. The ratio of solid matter to dry resin is 1:6, and the ratio of diameter to height is 1:8. First wash the resin column bed with 4 BV of distilled wa...

Embodiment 2

[0021] Example 2 Heated reflux extraction combined with macroporous adsorption resin column chromatography and high-speed countercurrent chromatography to prepare high-purity clove leaves total iridoid glycosides extract:

[0022] Add 15 times the amount of ethanol with a volume percentage concentration of 80% to 2 kg of clove leaves crushed to 20-30 mesh, heat and reflux for extraction three times, each time for 1.5 h, filter, combine the extracts, and concentrate to a concentration of 1.0 g Crude drug / ml, after filtering, add 1 mol / L hydrochloric acid to adjust the pH value to 4-5, pass through the D101 macroporous adsorption resin chromatography column at a flow rate of 3 BV / h, and the ratio of the sample solution to the dry resin is 1:8, the diameter-to-height ratio is 1:10, first wash the resin with 6 BV of water, the elution flow rate is 4 BV / h, discard the water eluent; then elute with 60% methanol by volume, The elution flow rate is 3 BV / h, and the eluent is decompress...

Embodiment 3

[0023] Example 3 Heated reflux extraction combined with macroporous adsorption resin column chromatography and dynamic axial compression industrial preparation chromatographic separation technology to prepare high-purity clove leaves total iridoid glycosides extract:

[0024] 5 kg of clove leaves are moderately crushed, add 10 times the amount of 60% methanol, and extract 3 times at a frequency of 30 kHz and ultrasonic power of 20 kW, 0.5 h each time, filter, combine the extracts, recover methanol under reduced pressure, and add water to adjust the concentration to 1.5 g crude drug / ml, add 1 mol / L hydrochloric acid to adjust the pH value to 6, and pass through the HPD400 macroporous adsorption resin chromatography column twice at a flow rate of 2BV / h. The ratio of the loading sample solution to the dry resin is 1:9, the diameter-to-height ratio is 1:8, first wash the resin with 4 BV of water, the elution flow rate is 2BV / h, discard the water eluent; then elute with 70% ethanol ...

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Abstract

The invention provides preparation methods and applications of a high-purity total iridoid glycoside extract from folium syringae and preparations of the high-purity total iridoid glycoside extract from the folium syringae. Dynamic ultrasonic reflux extraction or heating reflux extraction is adopted, separation based on dynamic axial compression industrial chromatography or high-speed counter-current chromatography is adopted for preparation, freeze drying or spray drying is performed, and the high-purity total iridoid glycoside extract from the folium syringae is obtained. According to the method, extraction is finished at a lower temperature within short time, the separation cycle is short, the efficiency is high, extraction volume is saved, and production cost, energy consumption and environmental pollution are reduced. HPLC detection proves that the purity of total iridoid glycosides of the folium syringae can reach 75% or higher. Various preparations such as thin membrane coated tablets, capsules, dispersible tablets, dripping pills, granules, oral sustained and controlled release preparations, spray, aerosol, injection and the like are prepared through addition of various pharmaceutical excipients and used for treating infectious diseases such as upper respiratory infection, acute and chronic amygdalitis, acute and chronic trachitis, acute gastroenteritis, acute icterohepatitis, acute mastitis, bacillary dysentery and the like.

Description

technical field [0001] The invention belongs to the field of extracts of plants and traditional Chinese medicines, and in particular relates to a preparation method and application of a high-purity clove leaf total iridoid glycoside extract and a preparation thereof. Background technique [0002] Bacterial infectious inflammation is a high-incidence and universal disease, which is mainly treated with antibacterial and anti-inflammatory methods in clinic. As the main drug for the treatment of bacterial infectious diseases, antibiotics are the most widely used, fastest growing and most diverse class of drugs in the world. The survey results show that in developed countries in Europe and the United States, the use of antibiotics accounts for about 10% of all drugs, while in my country, the tertiary hospitals account for 30%, and the grassroots hospitals may be as high as 50%. This shows that the frequency of antibiotic use in my country is very high. High 【http: / / drug.sosyao.co...

Claims

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Application Information

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IPC IPC(8): A61K36/63A61K9/30A61K9/22A61P31/04A61P11/00A61P1/12A61P1/00A61P1/04A61P1/16A61P31/14A61P15/14A61K127/00
CPCA61K9/2031A61K9/2059A61K9/28A61K36/63A61K2236/33A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55Y02A50/30
Inventor 刘欣孙晓君马慧媛张凤鸣庞海军庞素艳
Owner HARBIN UNIV OF SCI & TECH
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