Heterotrophic culture method for microalgae by utilizing starch and immobilized microorganism co-culture

Abstract

The invention belongs to the biotechnology field and in particular relates to a heterotrophic culture method for microalgae by utilizing starch and immobilized microorganism co-culture. The method comprises the following steps: preparing a culture medium, inoculating, immobilizing microorganism cells, culturing, and collecting cells. The method provided by the invention has the main characteristics that co-culture is carried out on the microalgae and immobilized microorganisms with starch hydrolysis capability, the problem that the microalgae is difficult to be subjected to heterotrophic culture by utilizing a low-cost starch type raw material is solved, a new approach is provided for reducing carbon source cost of the heterotrophic culture of the microalgae, pure culture of the microalgae is realized by immobilizing co-culture microorganisms, and compared with the conventional co-culture technology, algae-bacteria separation is realized by virtue of simple filtration and purely cultured microalgae cells can be obtained finally, so that the method provided by the invention can be widely applicable to a production process of various algae-based biological products.

Description

technical field [0001] The invention specifically relates to a method for cultivating microalgae heterotrophically by starch through co-cultivation with immobilized microorganisms, and belongs to the field of biotechnology. Background technique [0002] The problem of light limitation in the autotrophic culture process of microalgae makes the design and operation of the photosynthetic reactor more difficult, and because the growth of cells is limited by light under autotrophic growth, the cell growth is usually slow, the culture period is long, and the final biological The lower biomass concentration will further increase the difficulty of the subsequent cell harvesting process. Some algae can grow heterotrophically using exogenous organic carbon sources under the condition of no light source. In heterotrophic culture, the addition of exogenous organic carbon sources can significantly promote the growth rate of cells in the microalgae culture process, greatly shortening the ...

AI Technical Summary

Problems solved by technology

Starch, as a kind of carbohydrate with wide sources and low price, is an ideal carbon source, but because it is difficult for microorganisms to directly utilize high-molecular starch, and many microorganisms including algae cannot secrete amylase, therefore, in many During the cultivation of microorganisms, it is difficult to directly use starch

At present, there are a few reports on the use of starch for microalgae heterotrophic / combinotrophic culture, but in the above studies, the starch needs to be hydrolyzed into glucose or oligosaccharides by chemical or enzymatic methods before being used in the culture of microalgae. The process increases the unit operation of the microalgae cultivation process, which in turn increases the production cost