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A dye anaerobic biological decolorization system and method

An anaerobic biological and dye technology, applied in water pollutants, anaerobic digestion treatment, sustainable biological treatment and other directions, can solve the problems of high cost of lactic acid, lack of macromolecular organic matter utilization ability, restricting practical application, etc. cost, solving the problem of carbon source cost, and the effect of efficient degradation

Active Publication Date: 2022-05-20
JIANGSU UNIV
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Problems solved by technology

[0004] However, the electrogenic microorganism MR-1 can only use small molecular organic matter such as lactic acid as a carbon source, and lacks the ability to utilize large molecular organic matter such as starch.
Due to the high cost of lactic acid, this largely restricts its practical application in the bioremediation of environmental pollutants.

Method used

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  • A dye anaerobic biological decolorization system and method
  • A dye anaerobic biological decolorization system and method
  • A dye anaerobic biological decolorization system and method

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Embodiment

[0039] A low-carbon source cost dye anaerobic biological decolorization system, brown rot fungus G. trabeum First, the starch is hydrolyzed to generate glucose, and the glucose is further metabolized into small molecular organic compounds such as ethanol. Shewanella MR-1 utilizes ethanol and other anaerobic respiration and metabolism to release electrons, and finally transfers electrons to dye pollutants to achieve efficient degradation of pollutants.

[0040] The specific implementation steps are as follows:

[0041] (1) Streak the MR-1 stored in glycerol on a solid plate LB medium (containing 5g / L of yeast extract, 10g / L of tryptone, 10g / L of sodium chloride, 2% agar) at 30°C Incubate overnight.

[0042] (2) Use a pipette to suck up the activated single colony, inoculate it in 50mL liquid LB medium, (30 ± 0.5) ℃, (200 ± 5) rpm and shake for 10 hours to the late logarithmic phase, and then in the late logarithmic phase The bacteria were collected by centrifugation at 7000...

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Abstract

The invention discloses a dye biological decolorization method with low carbon source cost and belongs to the technical field of sewage treatment. In the present invention, at first the electricity-producing microorganism MR-1 and the brown rot fungus G. trabeum Insert into the decolorization medium with starch as carbon source for anaerobic co-cultivation; during the cultivation process, the electrogenic microorganisms S. oneidensis MR‑1 utilizes brown rot fungi G. trabeum Perform anaerobic respiration on starch decomposition products to release electrons, and finally transfer electrons to organic pollutants, so that the pollutants can be degraded; the new degradation system established by the present invention can not only realize the efficient degradation of dye pollutants, but also reduce the cost of carbon sources It is cheap, and can even achieve zero cost of carbon source, which greatly improves the practical application potential of electrogenic microorganisms to remediate environmental pollutants.

Description

technical field [0001] The invention relates to a low-carbon source cost dye anaerobic biological decolorization method, which belongs to the technical field of sewage treatment. Background technique [0002] The dye wastewater has complex composition, poor biodegradability, toxicity and mutagenicity. Therefore, if the untreated dye wastewater is directly discharged into the environment, it will seriously threaten the safety of humans and aquatic organisms. In order to solve the problem of dye wastewater pollution, physical methods, chemical methods and biological methods have been developed to treat dye wastewater. Among them, physical and chemical methods have high treatment costs, especially secondary pollution, and their applications are limited. The biological method is considered to be the most promising method because of its relatively low cost, environmental friendliness, and good treatment effect, and has received extensive attention. [0003] Electrogenic microorg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/28C02F3/34C02F101/30
CPCC02F3/28C02F3/34C02F2101/308C02F2305/06Y02W10/10
Inventor 李潜胡瑞沈鑫董颉张雪峰肖翔
Owner JIANGSU UNIV
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