Micro-fluidic chip, system and method for sorting and enriching cells in cerebrospinal fluid

Abstract

The invention belongs to the technical field of biochemical separation and analysis, and particularly relates to a micro-fluidic chip, a system and a method for sorting and enriching cells in cerebrospinal fluid. The chip can avoid pretreatment operations such as sample pretreatment and cell labeling and realize fast and efficient sorting and enriching functions for a small quantity of cells in the cerebrospinal fluid and further can realize in-situ counting and fast distinguishing of the small quantity of cells in the cerebrospinal fluid in combination with an in-situ microtechnique. The system integrates fast and efficient separation and enrichment of the small quantity of cells in the cerebrospinal fluid and in-situ microscopic observation, can realize sorting, enrichment, counting, observation and detection for the small quantity of cells in the cerebrospinal fluid, is high in functionality and high in efficiency, and can be used for clinical fast and efficient detection for cerebrospinal fluid cells.

Description

technical field [0001] The invention belongs to the technical field of biochemical separation and analysis, and in particular relates to a microfluidic chip, system and method for sorting and enriching cells in cerebrospinal fluid. Background technique [0002] Cerebrospinal fluid is traditionally also known as cerebrospinal fluid (Cerebro Spinal Fluid, CSF), which exists in the subarachnoid space and the chamber surrounding and passing through the central nervous system (CNS). It is the extracellular fluid of the central nervous system. Some of the ingredients are salt, glucose, protein and water. The type and quantity of cells in normal cerebrospinal fluid are rare, but the type and quantity of white blood cells and other cells in the cerebrospinal fluid of patients are significantly different from those in normal cerebrospinal fluid. Therefore, sorting, counting, identification and monitoring of cells in cerebrospinal fluid has become a routine testing item in basic labo...

AI Technical Summary

Problems solved by technology

[0005] In view of the defects of small amount of cells in cerebrospinal fluid, difficulty in obtaining, low sorting efficiency, and lack of rapid and effective analysis and enrichment methods in clinical practice, one purpose of the present invention is to provide a microfluidic chip for cell sorting and enrichment in cerebrospinal fluid , the chip can avoid pretreatment operations such as sample pretreatment and cell labeling, and realize the rapid and efficient sorting and enrichment function of small amount of cells in cerebrospinal fluid, and can further combine in situ microscopy technology to realize the detection of small amount of cells in cerebrospinal fluid In situ counting and rapid identification

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