Bioactive peptide and preparation method thereof

Abstract

The invention discloses a bioactive peptide and a preparation method thereof. The preparation method comprises the following steps: using milk or casein as a raw material, selecting an appropriate protease, carrying out enzymatic hydrolysis under certain conditions, carrying out concentration by adopting a membrane technology, desalting the obtained concentrate in a reverse osmosis manner, and drying the desalted concentrate by adopting a vacuum freeze-drying technique or a low-temperature spray drying technique in order to prepare polypeptide powder rich in bioactive peptides; and screening the polypeptides having anticoagulation activity through molecular fishing, sequencing the screened polypeptides to obtain an amino acid sequence, and synthesizing the final highly-pure polypeptide product in a solid phase synthesis manner. An identification result shows that the angiotensin converting enzyme inhibition rate and the thrombin inhibiting peptide inhibition rate of the peptide disclosed in the invention are high, the angiotensin converting enzyme inhibition rate of 1 mg of antithrombotic peptide dry powder is 30-80%, and the thrombin inhibition rate of 1 mg of the antithrombotic peptide dry powder is 70-98%. The method for preparing the bioactive peptide from the milk or casein has the advantages of richness in the raw material, low price, and meeting of large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of food-derived bioactive peptide development, and in particular relates to a preparation method for preparing a polypeptide with blood pressure lowering and antithrombotic activities by using milk or casein as a raw material. Background technique [0002] Due to the high work pressure, fast pace of life of modern people, and the influence of bad living habits such as lack of sleep, overeating, smoking and alcoholism, thrombosis in cardiovascular and cerebrovascular diseases has become the number one killer of human beings. Vascular disease causes millions of premature deaths every year in Europe, and the epidemic trend of cardiovascular disease in my country is becoming more and more significant. According to the survey, the prevalence of cardiovascular diseases in my country is 1.78% for men and 1.10% for women, and the prevalence increases with age. Nearly 3.5 million people die of cardiovascular diseases ...

AI Technical Summary

Problems solved by technology

[0009] At present, there are few reports on polypeptides with hypotensive and antithrombotic activities obtained by various methods. Only in 2012, Rojas–Ronquillo et al. identified both hypotensive and antithrombotic activities from β-casein through fermented cow milk casein Peptide 17 YQEPVLGPVRGPFPIIV (aa192-209)